Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas

José Carlos López López,1 Nieves Fernández Alonso,1 Juan Cuevas Álvarez,1,2 Tomás García-Caballero,2 José Carlos Pastor Jimeno3 1Ocular Pathology Laboratory, Instituto Universitario de Oftalmobiología...

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Autores principales:	López López JC, Fernández Alonso N, Cuevas Álvarez J, García-Caballero T, Pastor Jimeno JC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Retinoblastoma immunohistochemistry neuron-specific enolase synaptophysin pRb Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/c53f7e1e67d7471fb0d0859df2e2ca5a
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spelling	oai:doaj.org-article:c53f7e1e67d7471fb0d0859df2e2ca5a2021-12-02T07:06:46ZImmunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas1177-5483https://doaj.org/article/c53f7e1e67d7471fb0d0859df2e2ca5a2018-06-01T00:00:00Zhttps://www.dovepress.com/immunohistochemical-assay-for-neuron-specific-enolase-synaptophysin-an-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483José Carlos López López,1 Nieves Fernández Alonso,1 Juan Cuevas Álvarez,1,2 Tomás García-Caballero,2 José Carlos Pastor Jimeno3 1Ocular Pathology Laboratory, Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain; 2Department of Pathology, Hospital Clínico Universitario, Santiago de Compostela, Spain; 3Retina Group, Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Hospital Clínico Universitario, Valladolid, Spain Purpose: We evaluated the expression of the neural markers, neuron-specific enolase, and synaptophysin, as a tool to confirm the diagnosis of retinoblastoma (RB) in undifferentiated and advanced tumors. Additionally, we determined whether the extent of RB-associated protein (pRb) expression is helpful in assessing the prognosis in RB patients. Methods: Conventional whole tissue section and tissue microarray immunohistochemistry for neuron-specific enolase, synaptophysin, and pRb were carried out in a series of 22 RBs. Results: Neuron-specific enolase and synaptophysin were expressed in 75%–100% of the tumor cells, and the staining intensity was strong. Two RBs expressed pRb in 75%–100% of the tumor cells, also with strong staining intensity. Concordance between the immunohistochemical outcomes for whole tissue staining and tissue microarray staining was 76.2% for neuron-specific enolase, 85.7% for synaptophysin, and 80.0% for pRb. Conclusion: Neuron-specific enolase and synaptophysin have the potential to be useful markers for the diagnosis of RBs. Extensive and strong pRb staining is not associated with less aggressive tumor behavior according to the pathologic classification of RBs. Keywords: retinoblastoma, immunohistochemistry, neuron-specific enolase, synaptophysin, pRbLópez López JCFernández Alonso NCuevas Álvarez JGarcía-Caballero TPastor Jimeno JCDove Medical PressarticleRetinoblastomaimmunohistochemistryneuron-specific enolasesynaptophysinpRbOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 1171-1179 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Retinoblastoma immunohistochemistry neuron-specific enolase synaptophysin pRb Ophthalmology RE1-994
spellingShingle	Retinoblastoma immunohistochemistry neuron-specific enolase synaptophysin pRb Ophthalmology RE1-994 López López JC Fernández Alonso N Cuevas Álvarez J García-Caballero T Pastor Jimeno JC Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
description	José Carlos López López,1 Nieves Fernández Alonso,1 Juan Cuevas Álvarez,1,2 Tomás García-Caballero,2 José Carlos Pastor Jimeno3 1Ocular Pathology Laboratory, Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain; 2Department of Pathology, Hospital Clínico Universitario, Santiago de Compostela, Spain; 3Retina Group, Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Hospital Clínico Universitario, Valladolid, Spain Purpose: We evaluated the expression of the neural markers, neuron-specific enolase, and synaptophysin, as a tool to confirm the diagnosis of retinoblastoma (RB) in undifferentiated and advanced tumors. Additionally, we determined whether the extent of RB-associated protein (pRb) expression is helpful in assessing the prognosis in RB patients. Methods: Conventional whole tissue section and tissue microarray immunohistochemistry for neuron-specific enolase, synaptophysin, and pRb were carried out in a series of 22 RBs. Results: Neuron-specific enolase and synaptophysin were expressed in 75%–100% of the tumor cells, and the staining intensity was strong. Two RBs expressed pRb in 75%–100% of the tumor cells, also with strong staining intensity. Concordance between the immunohistochemical outcomes for whole tissue staining and tissue microarray staining was 76.2% for neuron-specific enolase, 85.7% for synaptophysin, and 80.0% for pRb. Conclusion: Neuron-specific enolase and synaptophysin have the potential to be useful markers for the diagnosis of RBs. Extensive and strong pRb staining is not associated with less aggressive tumor behavior according to the pathologic classification of RBs. Keywords: retinoblastoma, immunohistochemistry, neuron-specific enolase, synaptophysin, pRb
format	article
author	López López JC Fernández Alonso N Cuevas Álvarez J García-Caballero T Pastor Jimeno JC
author_facet	López López JC Fernández Alonso N Cuevas Álvarez J García-Caballero T Pastor Jimeno JC
author_sort	López López JC
title	Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
title_short	Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
title_full	Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
title_fullStr	Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
title_full_unstemmed	Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas
title_sort	immunohistochemical assay for neuron-specific enolase, synaptophysin, and rb-associated protein as a diagnostic aid in advanced retinoblastomas
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c53f7e1e67d7471fb0d0859df2e2ca5a
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Immunohistochemical assay for neuron-specific enolase, synaptophysin, and RB-associated protein as a diagnostic aid in advanced retinoblastomas

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