White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population

Abstract Several studies have demonstrated that injection of double-stranded RNAs (dsRNA) homologous to mRNA for the white spot syndrome virus (WSSV) viral protein 28 (VP28) can induce protection in shrimp against WSSV through RNA interference (RNAi). In comparison to shrimp injected with either PBS...

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Autores principales:	Pål Nilsen, Marius Karlsen, Kallaya Sritunyalucksana, Siripong Thitamadee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c54ae807845a4dfd8b967b2abedf0dfc
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spelling	oai:doaj.org-article:c54ae807845a4dfd8b967b2abedf0dfc2021-12-02T16:07:03ZWhite spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population10.1038/s41598-017-01181-w2045-2322https://doaj.org/article/c54ae807845a4dfd8b967b2abedf0dfc2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01181-whttps://doaj.org/toc/2045-2322Abstract Several studies have demonstrated that injection of double-stranded RNAs (dsRNA) homologous to mRNA for the white spot syndrome virus (WSSV) viral protein 28 (VP28) can induce protection in shrimp against WSSV through RNA interference (RNAi). In comparison to shrimp injected with either PBS or a green fluorescent protein (GFP) nonspecific dsRNA, we obtained nearly complete protection against WSSV infection in shrimp injected with VP28 dsRNA. Upregulation of host genes associated with small RNA silencing was measured 48 hours post treatment in groups injected with dsRNA, and although the VP28-treated group remained moderately upregulated after challenge with WSSV, many-fold higher induction was observed in both control groups reflecting the ongoing viral infection. RNA sequencing of VP28-treated shrimp demonstrated a siRNA population dominated by high levels of 22 nt long molecules narrowly targeting the VP28 mRNA both before and after challenge with WSSV. Conversely, while no siRNAs targeting WSSV were detected before challenge, a broad response of 22 nt siRNAs mapping across the entire WSSV genome were found in both control groups after challenge. These results give detailed insight to how dsRNA targeting VP28 function to induce protection against WSSV, by generating a highly focused population of 22 nt long siRNA molecules.Pål NilsenMarius KarlsenKallaya SritunyalucksanaSiripong ThitamadeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pål Nilsen Marius Karlsen Kallaya Sritunyalucksana Siripong Thitamadee White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
description	Abstract Several studies have demonstrated that injection of double-stranded RNAs (dsRNA) homologous to mRNA for the white spot syndrome virus (WSSV) viral protein 28 (VP28) can induce protection in shrimp against WSSV through RNA interference (RNAi). In comparison to shrimp injected with either PBS or a green fluorescent protein (GFP) nonspecific dsRNA, we obtained nearly complete protection against WSSV infection in shrimp injected with VP28 dsRNA. Upregulation of host genes associated with small RNA silencing was measured 48 hours post treatment in groups injected with dsRNA, and although the VP28-treated group remained moderately upregulated after challenge with WSSV, many-fold higher induction was observed in both control groups reflecting the ongoing viral infection. RNA sequencing of VP28-treated shrimp demonstrated a siRNA population dominated by high levels of 22 nt long molecules narrowly targeting the VP28 mRNA both before and after challenge with WSSV. Conversely, while no siRNAs targeting WSSV were detected before challenge, a broad response of 22 nt siRNAs mapping across the entire WSSV genome were found in both control groups after challenge. These results give detailed insight to how dsRNA targeting VP28 function to induce protection against WSSV, by generating a highly focused population of 22 nt long siRNA molecules.
format	article
author	Pål Nilsen Marius Karlsen Kallaya Sritunyalucksana Siripong Thitamadee
author_facet	Pål Nilsen Marius Karlsen Kallaya Sritunyalucksana Siripong Thitamadee
author_sort	Pål Nilsen
title	White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
title_short	White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
title_full	White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
title_fullStr	White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
title_full_unstemmed	White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
title_sort	white spot syndrome virus vp28 specific double-stranded rna provides protection through a highly focused sirna population
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c54ae807845a4dfd8b967b2abedf0dfc
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White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population

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