Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs

Cellular communications take place thanks to a well-connected network of chemical–physical signals, biomolecules, growth factors, and vesicular messengers that travel inside or between cells. A deep knowledge of the extracellular vesicle (EV) system allows for a better understanding of the whole ser...

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Main Authors:	Tania Limongi, Francesca Susa, Bianca Dumontel, Luisa Racca, Michela Perrone Donnorso, Doriana Debellis, Valentina Cauda
Format:	article
Language:	EN
Published:	MDPI AG 2021
Subjects:	extracellular vesicles B lymphocytes human myeloid leukemia Burkitt lymphoma surface labeling cellular uptake Chemical technology TP1-1185 Chemical engineering TP155-156
Online Access:	https://doaj.org/article/c54ee43f9ffd4030b0e03cb3f897e15a
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spelling	oai:doaj.org-article:c54ee43f9ffd4030b0e03cb3f897e15a2021-11-25T18:20:04ZExtracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs10.3390/membranes111108862077-0375https://doaj.org/article/c54ee43f9ffd4030b0e03cb3f897e15a2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/886https://doaj.org/toc/2077-0375Cellular communications take place thanks to a well-connected network of chemical–physical signals, biomolecules, growth factors, and vesicular messengers that travel inside or between cells. A deep knowledge of the extracellular vesicle (EV) system allows for a better understanding of the whole series of phenomena responsible for cell proliferation and death. To this purpose, here, a thorough immuno-phenotypic characterization of B-cell EV membranes is presented. Furthermore, the cellular membrane of B lymphocytes, Burkitt lymphoma, and human myeloid leukemic cells were characterized through cytofluorimetry assays and fluorescent microscopy analysis. Through cytotoxicity and internalization tests, the tropism of B lymphocyte-derived EVs was investigated toward the parental cell line and two different cancer cell lines. In this study, an innate capability of passive targeting of the native EVs was distinguished from the active targeting capability of monoclonal antibody-engineered EVs, able to selectively drive the vesicles, enhancing their internalization into the target cancer cells. In particular, the specific targeting ability of anti-CD20 engineered EVs towards Daudi cells, highly expressing CD20 marker on their cell membrane, was proved, while almost no internalization events were observed in HL60 cells, since they did not express an appreciable amount of the CD20 marker on their plasma membranes.Tania LimongiFrancesca SusaBianca DumontelLuisa RaccaMichela Perrone DonnorsoDoriana DebellisValentina CaudaMDPI AGarticleextracellular vesiclesB lymphocyteshuman myeloid leukemiaBurkitt lymphomasurface labelingcellular uptakeChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 886, p 886 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles B lymphocytes human myeloid leukemia Burkitt lymphoma surface labeling cellular uptake Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	extracellular vesicles B lymphocytes human myeloid leukemia Burkitt lymphoma surface labeling cellular uptake Chemical technology TP1-1185 Chemical engineering TP155-156 Tania Limongi Francesca Susa Bianca Dumontel Luisa Racca Michela Perrone Donnorso Doriana Debellis Valentina Cauda Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
description	Cellular communications take place thanks to a well-connected network of chemical–physical signals, biomolecules, growth factors, and vesicular messengers that travel inside or between cells. A deep knowledge of the extracellular vesicle (EV) system allows for a better understanding of the whole series of phenomena responsible for cell proliferation and death. To this purpose, here, a thorough immuno-phenotypic characterization of B-cell EV membranes is presented. Furthermore, the cellular membrane of B lymphocytes, Burkitt lymphoma, and human myeloid leukemic cells were characterized through cytofluorimetry assays and fluorescent microscopy analysis. Through cytotoxicity and internalization tests, the tropism of B lymphocyte-derived EVs was investigated toward the parental cell line and two different cancer cell lines. In this study, an innate capability of passive targeting of the native EVs was distinguished from the active targeting capability of monoclonal antibody-engineered EVs, able to selectively drive the vesicles, enhancing their internalization into the target cancer cells. In particular, the specific targeting ability of anti-CD20 engineered EVs towards Daudi cells, highly expressing CD20 marker on their cell membrane, was proved, while almost no internalization events were observed in HL60 cells, since they did not express an appreciable amount of the CD20 marker on their plasma membranes.
format	article
author	Tania Limongi Francesca Susa Bianca Dumontel Luisa Racca Michela Perrone Donnorso Doriana Debellis Valentina Cauda
author_facet	Tania Limongi Francesca Susa Bianca Dumontel Luisa Racca Michela Perrone Donnorso Doriana Debellis Valentina Cauda
author_sort	Tania Limongi
title	Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
title_short	Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
title_full	Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
title_fullStr	Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
title_full_unstemmed	Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs
title_sort	extracellular vesicles tropism: a comparative study between passive innate tropism and the active engineered targeting capability of lymphocyte-derived evs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c54ee43f9ffd4030b0e03cb3f897e15a
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Extracellular Vesicles Tropism: A Comparative Study between Passive Innate Tropism and the Active Engineered Targeting Capability of Lymphocyte-Derived EVs

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