Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

Micropropagation protocol of Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments (Basal and Tip) as explants. Surface sterilization of healthy bulb scales with carbendazim 200 ppm for 30 min, then 0.1 percent mercuric chloride for 10 min, then 70% ethyl alcohol for 30 s was su...

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Autores principales:	Sadaf Rafiq, Z.A. Rather, Reyaz Ahmad Bhat, I.T. Nazki, Mohammad S. AL-Harbi, Neelofar Banday, Iqra Farooq, Bassem N. Samra, M.H. Khan, Atef F. Ahmed, Najmah Andrabi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bulb scale Lilium Micropropagation Ravenna Tissue culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c551dff5bd22486db8e1099b0e885d09
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spelling	oai:doaj.org-article:c551dff5bd22486db8e1099b0e885d092021-11-20T04:57:23ZStandardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’1319-562X10.1016/j.sjbs.2021.09.064https://doaj.org/article/c551dff5bd22486db8e1099b0e885d092021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21008676https://doaj.org/toc/1319-562XMicropropagation protocol of Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments (Basal and Tip) as explants. Surface sterilization of healthy bulb scales with carbendazim 200 ppm for 30 min, then 0.1 percent mercuric chloride for 10 min, then 70% ethyl alcohol for 30 s was superior to all other treatments in recording highest culture asepsis (77.08%) and higher explant survival (86.12%). Explant survival was higher in basal segments (88.54%) compared to tip segments (85.52%). Highest culture establishment was recorded in basal scale segments (68.26%) followed by tip scale segments (55.21%). MS medium augmented with 0.50 mgl−1 Naphthalene acetic acid and 2.0 mgl−1. 6-Benzylamino Purine recorded maximum culture establishment (76.17%), highest bulblet number/explant (5.52) with maximum length of shoots (2.20 cm) and number of leaves (3.39). This treatment combination of growth regulators resulted in highest shoot proliferation (83.33%) along with maximum shoot number (2.41explant−1), shoot length (2.35 cm) and leaf number (5.44) of micro shoots during proliferation stage. Rooting of explants was superior with Indole-3-butyric acid compared to Naphthalene acetic acid. Highest rooting of 92.71% along with maximum number of primary roots shoot−1 (12.06), maximum primary root length (3.17 cm) was documented in Murashige and Skoog medium added with Indole-3-butyric acid 1.50 mgl−1 with best ex vitro survival rate (98.96%) of rooted plantlets during primary hardening in perlite + vermiculite (1:1) mixture.Sadaf RafiqZ.A. RatherReyaz Ahmad BhatI.T. NazkiMohammad S. AL-HarbiNeelofar BandayIqra FarooqBassem N. SamraM.H. KhanAtef F. AhmedNajmah AndrabiElsevierarticleBulb scaleLiliumMicropropagationRavennaTissue cultureBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7581-7587 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bulb scale Lilium Micropropagation Ravenna Tissue culture Biology (General) QH301-705.5
spellingShingle	Bulb scale Lilium Micropropagation Ravenna Tissue culture Biology (General) QH301-705.5 Sadaf Rafiq Z.A. Rather Reyaz Ahmad Bhat I.T. Nazki Mohammad S. AL-Harbi Neelofar Banday Iqra Farooq Bassem N. Samra M.H. Khan Atef F. Ahmed Najmah Andrabi Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
description	Micropropagation protocol of Oriental Hybrid Lilium cv. Ravenna was developed using bulb scale segments (Basal and Tip) as explants. Surface sterilization of healthy bulb scales with carbendazim 200 ppm for 30 min, then 0.1 percent mercuric chloride for 10 min, then 70% ethyl alcohol for 30 s was superior to all other treatments in recording highest culture asepsis (77.08%) and higher explant survival (86.12%). Explant survival was higher in basal segments (88.54%) compared to tip segments (85.52%). Highest culture establishment was recorded in basal scale segments (68.26%) followed by tip scale segments (55.21%). MS medium augmented with 0.50 mgl−1 Naphthalene acetic acid and 2.0 mgl−1. 6-Benzylamino Purine recorded maximum culture establishment (76.17%), highest bulblet number/explant (5.52) with maximum length of shoots (2.20 cm) and number of leaves (3.39). This treatment combination of growth regulators resulted in highest shoot proliferation (83.33%) along with maximum shoot number (2.41explant−1), shoot length (2.35 cm) and leaf number (5.44) of micro shoots during proliferation stage. Rooting of explants was superior with Indole-3-butyric acid compared to Naphthalene acetic acid. Highest rooting of 92.71% along with maximum number of primary roots shoot−1 (12.06), maximum primary root length (3.17 cm) was documented in Murashige and Skoog medium added with Indole-3-butyric acid 1.50 mgl−1 with best ex vitro survival rate (98.96%) of rooted plantlets during primary hardening in perlite + vermiculite (1:1) mixture.
format	article
author	Sadaf Rafiq Z.A. Rather Reyaz Ahmad Bhat I.T. Nazki Mohammad S. AL-Harbi Neelofar Banday Iqra Farooq Bassem N. Samra M.H. Khan Atef F. Ahmed Najmah Andrabi
author_facet	Sadaf Rafiq Z.A. Rather Reyaz Ahmad Bhat I.T. Nazki Mohammad S. AL-Harbi Neelofar Banday Iqra Farooq Bassem N. Samra M.H. Khan Atef F. Ahmed Najmah Andrabi
author_sort	Sadaf Rafiq
title	Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
title_short	Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
title_full	Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
title_fullStr	Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
title_full_unstemmed	Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’
title_sort	standardization of in vitro micropropagation procedure of oriental lilium hybrid cv. ‘ravenna’
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c551dff5bd22486db8e1099b0e885d09
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Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. ‘Ravenna’

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