Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.

Pancreatic islets from adult rats whose mothers were protein restricted during lactation undersecrete insulin. The current work analyzes whether this secretory dysfunction can be improved when the pancreatic islets are grafted into hyperglycemic diabetic rats. Two groups of rats were used: the adult...

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Autores principales:	Renato Chaves Souto Branco, Júlio Cezar de Oliveira, Sabrina Grassiolli, Rosiane Aparecida Miranda, Luiz Felipe Barella, Rodrigo Mello Gomes, Luiz Augusto Bataglini, Rosana Torrezan, Clarice Gravena, Paulo Cezar de Freitas Mathias
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5565741a9fa49709fdfc1a13f2c919e
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spelling	oai:doaj.org-article:c5565741a9fa49709fdfc1a13f2c919e2021-11-18T07:26:45ZMaternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.1932-620310.1371/journal.pone.0030685https://doaj.org/article/c5565741a9fa49709fdfc1a13f2c919e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22383969/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pancreatic islets from adult rats whose mothers were protein restricted during lactation undersecrete insulin. The current work analyzes whether this secretory dysfunction can be improved when the pancreatic islets are grafted into hyperglycemic diabetic rats. Two groups of rats were used: the adult offspring from dams that received a low protein diet (4%) during the initial 2/3 of lactation (LP) and, as a control, the adult offspring from dams that consumed a normal protein diet (23%) during the entire period of lactation (NP). Islets from NP- and LP-rats were transplanted into diabetic recipient rats, which were generated by streptozotocin treatment. The islets were transplanted via the portal vein under anesthesia. The fed blood glucose levels were monitored during the 4 days post-transplantation. Transplanted islets from LP-rats (T LP) decreased the fed glucose levels of diabetic rats 34% (21.37 ± 0.24 mM, p<0.05); however, the levels still remained 2-fold higher than those of the sham-operated controls (6.88 ± 0.39 mM, p<0.05). Grafts with NP-islets (T NP) produced the same effect as the LP-islets in diabetic rats. The high fasting blood glucose levels of diabetic rats were improved by the transplantations. Islet grafts from both rat groups recovered 50% of the retroperitoneal fat mass of the diabetic rats (0.55 ± 0.08 g/100 g of body weight for T NP and 0.56 ± 0.07 g/100 g of body weight for T LP, p<0.05). Because pancreatic islets from both the NP- and LP-rats were able to regulate fasting blood glucose concentrations in hyperglycemic rats, we propose that the altered function of pancreatic islets from LP-rats is not permanent.Renato Chaves Souto BrancoJúlio Cezar de OliveiraSabrina GrassiolliRosiane Aparecida MirandaLuiz Felipe BarellaRodrigo Mello GomesLuiz Augusto BatagliniRosana TorrezanClarice GravenaPaulo Cezar de Freitas MathiasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30685 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Renato Chaves Souto Branco Júlio Cezar de Oliveira Sabrina Grassiolli Rosiane Aparecida Miranda Luiz Felipe Barella Rodrigo Mello Gomes Luiz Augusto Bataglini Rosana Torrezan Clarice Gravena Paulo Cezar de Freitas Mathias Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
description	Pancreatic islets from adult rats whose mothers were protein restricted during lactation undersecrete insulin. The current work analyzes whether this secretory dysfunction can be improved when the pancreatic islets are grafted into hyperglycemic diabetic rats. Two groups of rats were used: the adult offspring from dams that received a low protein diet (4%) during the initial 2/3 of lactation (LP) and, as a control, the adult offspring from dams that consumed a normal protein diet (23%) during the entire period of lactation (NP). Islets from NP- and LP-rats were transplanted into diabetic recipient rats, which were generated by streptozotocin treatment. The islets were transplanted via the portal vein under anesthesia. The fed blood glucose levels were monitored during the 4 days post-transplantation. Transplanted islets from LP-rats (T LP) decreased the fed glucose levels of diabetic rats 34% (21.37 ± 0.24 mM, p<0.05); however, the levels still remained 2-fold higher than those of the sham-operated controls (6.88 ± 0.39 mM, p<0.05). Grafts with NP-islets (T NP) produced the same effect as the LP-islets in diabetic rats. The high fasting blood glucose levels of diabetic rats were improved by the transplantations. Islet grafts from both rat groups recovered 50% of the retroperitoneal fat mass of the diabetic rats (0.55 ± 0.08 g/100 g of body weight for T NP and 0.56 ± 0.07 g/100 g of body weight for T LP, p<0.05). Because pancreatic islets from both the NP- and LP-rats were able to regulate fasting blood glucose concentrations in hyperglycemic rats, we propose that the altered function of pancreatic islets from LP-rats is not permanent.
format	article
author	Renato Chaves Souto Branco Júlio Cezar de Oliveira Sabrina Grassiolli Rosiane Aparecida Miranda Luiz Felipe Barella Rodrigo Mello Gomes Luiz Augusto Bataglini Rosana Torrezan Clarice Gravena Paulo Cezar de Freitas Mathias
author_facet	Renato Chaves Souto Branco Júlio Cezar de Oliveira Sabrina Grassiolli Rosiane Aparecida Miranda Luiz Felipe Barella Rodrigo Mello Gomes Luiz Augusto Bataglini Rosana Torrezan Clarice Gravena Paulo Cezar de Freitas Mathias
author_sort	Renato Chaves Souto Branco
title	Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
title_short	Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
title_full	Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
title_fullStr	Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
title_full_unstemmed	Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
title_sort	maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c5565741a9fa49709fdfc1a13f2c919e
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Maternal protein malnutrition does not impair insulin secretion from pancreatic islets of offspring after transplantation into diabetic rats.

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