Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform

Abstract The zebrafish sperm activation profoundly depends upon the homogeneous mixing of the sperm cells with its diluent in a quick succession as it alters the cell’s extracellular medium and initiates their motility. Manual stirring, the traditional method for zebrafish sperm activation is tediou...

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Autores principales:	Bivas Panigrahi, Chang-Hung Lu, Neha Ghayal, Chia-Yuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c55698035cc34bcca85fe6deadcd8828
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spelling	oai:doaj.org-article:c55698035cc34bcca85fe6deadcd88282021-12-02T15:09:07ZSperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform10.1038/s41598-018-22563-82045-2322https://doaj.org/article/c55698035cc34bcca85fe6deadcd88282018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22563-8https://doaj.org/toc/2045-2322Abstract The zebrafish sperm activation profoundly depends upon the homogeneous mixing of the sperm cells with its diluent in a quick succession as it alters the cell’s extracellular medium and initiates their motility. Manual stirring, the traditional method for zebrafish sperm activation is tedious, time-consuming, and has a poor outcome. In this aspect, an artificial cilia embedded serpentine microfluidic is designed through which hydrodynamic factors of the microfluidic environment can be precisely regulated to harness uniform mixing, hence ensuring a superior sperm activation. To quantify the sperm motility, computer assisted sperm analysis software (CASA) was used whereas to quantify the generated flow field, micro particle image velocimetry (μPIV) was used. With this proposed microfluidic, 74.4% of the zebrafish sperm were activated which is 20% higher than its currently existing manual measurements. The μPIV analysis demonstrates that the curvature of the microchannel induces an orbital rotation to the flow field along the length of the microchannel together with the artificial cilia actuation which instigates a local rotation to the flow field of the artificial cilia location. The collective rotation in the whole flow field induce vorticity that promotes the change in temporal dynamics of the sperm cells towards their activation.Bivas PanigrahiChang-Hung LuNeha GhayalChia-Yuan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bivas Panigrahi Chang-Hung Lu Neha Ghayal Chia-Yuan Chen Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
description	Abstract The zebrafish sperm activation profoundly depends upon the homogeneous mixing of the sperm cells with its diluent in a quick succession as it alters the cell’s extracellular medium and initiates their motility. Manual stirring, the traditional method for zebrafish sperm activation is tedious, time-consuming, and has a poor outcome. In this aspect, an artificial cilia embedded serpentine microfluidic is designed through which hydrodynamic factors of the microfluidic environment can be precisely regulated to harness uniform mixing, hence ensuring a superior sperm activation. To quantify the sperm motility, computer assisted sperm analysis software (CASA) was used whereas to quantify the generated flow field, micro particle image velocimetry (μPIV) was used. With this proposed microfluidic, 74.4% of the zebrafish sperm were activated which is 20% higher than its currently existing manual measurements. The μPIV analysis demonstrates that the curvature of the microchannel induces an orbital rotation to the flow field along the length of the microchannel together with the artificial cilia actuation which instigates a local rotation to the flow field of the artificial cilia location. The collective rotation in the whole flow field induce vorticity that promotes the change in temporal dynamics of the sperm cells towards their activation.
format	article
author	Bivas Panigrahi Chang-Hung Lu Neha Ghayal Chia-Yuan Chen
author_facet	Bivas Panigrahi Chang-Hung Lu Neha Ghayal Chia-Yuan Chen
author_sort	Bivas Panigrahi
title	Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
title_short	Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
title_full	Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
title_fullStr	Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
title_full_unstemmed	Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
title_sort	sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c55698035cc34bcca85fe6deadcd8828
work_keys_str_mv	AT bivaspanigrahi spermactivationthroughorbitalandselfaxisrevolutionsusinganartificialciliaembeddedserpentinemicrofluidicplatform AT changhunglu spermactivationthroughorbitalandselfaxisrevolutionsusinganartificialciliaembeddedserpentinemicrofluidicplatform AT nehaghayal spermactivationthroughorbitalandselfaxisrevolutionsusinganartificialciliaembeddedserpentinemicrofluidicplatform AT chiayuanchen spermactivationthroughorbitalandselfaxisrevolutionsusinganartificialciliaembeddedserpentinemicrofluidicplatform
_version_	1718387901533782016

Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform

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