Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment

Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment

Philip JT Reardon,1,* Maryam Parhizkar,2,* Anthony H Harker,3 Richard J Browning,4 Vessela Vassileva,5 Eleanor Stride,4 R Barbara Pedley,5 Mohan Edirisinghe,2 Jonathan C Knowles1 1Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, 2Department of Mechanical Engineering, 3...

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Autores principales: Reardon PJT, Parhizkar M, Harker AH, Browning RJ, Vassileva V, Stride E, Pedley RB, Edirisinghe M, Knowles JC
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
cisplatin
drug delivery
cancer chemotherapy
polymer
Poly (lactic-co-glycolic acid
nanoparticles
electrohydrodynamic atomization
controlled release
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/c5577a17a8564e8d97fd258cd03393fe
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spelling oai:doaj.org-article:c5577a17a8564e8d97fd258cd03393fe2021-12-02T05:14:20ZElectrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment1178-2013https://doaj.org/article/c5577a17a8564e8d97fd258cd03393fe2017-05-01T00:00:00Zhttps://www.dovepress.com/electrohydrodynamic-fabrication-of-core-shell-plga-nanoparticles-with-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Philip JT Reardon,1,* Maryam Parhizkar,2,* Anthony H Harker,3 Richard J Browning,4 Vessela Vassileva,5 Eleanor Stride,4 R Barbara Pedley,5 Mohan Edirisinghe,2 Jonathan C Knowles1 1Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, 2Department of Mechanical Engineering, 3Department of Physics & Astronomy, University College London, London, 4Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, 5Department of Oncology, UCL Cancer Institute, University College London, London, UK *These authors contributed equally to this work Abstract: Increasing the clinical efficacy of toxic chemotherapy drugs such as cisplatin (CDDP), via targeted drug delivery, is a key area of research in cancer treatment. In this study, CDDP-loaded poly(lactic-co-glycolic acid) (PLGA) polymeric nanoparticles (NPs) were successfully prepared using electrohydrodynamic atomization (EHDA). The configuration was varied to control the distribution of CDDP within the particles, and high encapsulation efficiency (>70%) of the drug was achieved. NPs were produced with either a core–shell (CS) or a matrix (uniform) structure. It was shown that CS NPs had the most sustained release of the 2 formulations, demonstrating a slower linear release post initial “burst” and longer duration. The role of particle architecture on the rate of drug release in vitro was confirmed by fitting the experimental data with various kinetic models. This indicated that the release process was a simple diffusion mechanism. The CS NPs were effectively internalized into the endolysosomal compartments of cancer cells and demonstrated an increased cytotoxic efficacy (concentration of a drug that gives half maximal response [EC50] reaching 6.2 µM) compared to free drug (EC50 =9 µM) and uniform CDDP-distributed NPs (EC50 =7.6 µM) in vitro. Thus, these experiments indicate that engineering the structure of PLGA NPs can be exploited to control both the dosage and the release characteristics for improved clinical chemotherapy treatment. Keywords: cisplatin, drug delivery, cancer chemotherapy, polymer, poly(lactic-co-glycolic acid), nanoparticles, electrohydrodynamic atomization, controlled releaseReardon PJTParhizkar MHarker AHBrowning RJVassileva VStride EPedley RBEdirisinghe MKnowles JCDove Medical Pressarticlecisplatindrug deliverycancer chemotherapypolymerPoly (lactic-co-glycolic acidnanoparticleselectrohydrodynamic atomizationcontrolled releaseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3913-3926 (2017)
institution DOAJ
collection DOAJ
language EN
topic cisplatin
drug delivery
cancer chemotherapy
polymer
Poly (lactic-co-glycolic acid
nanoparticles
electrohydrodynamic atomization
controlled release
Medicine (General)
R5-920
spellingShingle cisplatin
drug delivery
cancer chemotherapy
polymer
Poly (lactic-co-glycolic acid
nanoparticles
electrohydrodynamic atomization
controlled release
Medicine (General)
R5-920
Reardon PJT
Parhizkar M
Harker AH
Browning RJ
Vassileva V
Stride E
Pedley RB
Edirisinghe M
Knowles JC
Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
description Philip JT Reardon,1,* Maryam Parhizkar,2,* Anthony H Harker,3 Richard J Browning,4 Vessela Vassileva,5 Eleanor Stride,4 R Barbara Pedley,5 Mohan Edirisinghe,2 Jonathan C Knowles1 1Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, 2Department of Mechanical Engineering, 3Department of Physics & Astronomy, University College London, London, 4Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, 5Department of Oncology, UCL Cancer Institute, University College London, London, UK *These authors contributed equally to this work Abstract: Increasing the clinical efficacy of toxic chemotherapy drugs such as cisplatin (CDDP), via targeted drug delivery, is a key area of research in cancer treatment. In this study, CDDP-loaded poly(lactic-co-glycolic acid) (PLGA) polymeric nanoparticles (NPs) were successfully prepared using electrohydrodynamic atomization (EHDA). The configuration was varied to control the distribution of CDDP within the particles, and high encapsulation efficiency (>70%) of the drug was achieved. NPs were produced with either a core–shell (CS) or a matrix (uniform) structure. It was shown that CS NPs had the most sustained release of the 2 formulations, demonstrating a slower linear release post initial “burst” and longer duration. The role of particle architecture on the rate of drug release in vitro was confirmed by fitting the experimental data with various kinetic models. This indicated that the release process was a simple diffusion mechanism. The CS NPs were effectively internalized into the endolysosomal compartments of cancer cells and demonstrated an increased cytotoxic efficacy (concentration of a drug that gives half maximal response [EC50] reaching 6.2 µM) compared to free drug (EC50 =9 µM) and uniform CDDP-distributed NPs (EC50 =7.6 µM) in vitro. Thus, these experiments indicate that engineering the structure of PLGA NPs can be exploited to control both the dosage and the release characteristics for improved clinical chemotherapy treatment. Keywords: cisplatin, drug delivery, cancer chemotherapy, polymer, poly(lactic-co-glycolic acid), nanoparticles, electrohydrodynamic atomization, controlled release
format article
author Reardon PJT
Parhizkar M
Harker AH
Browning RJ
Vassileva V
Stride E
Pedley RB
Edirisinghe M
Knowles JC
author_facet Reardon PJT
Parhizkar M
Harker AH
Browning RJ
Vassileva V
Stride E
Pedley RB
Edirisinghe M
Knowles JC
author_sort Reardon PJT
title Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
title_short Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
title_full Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
title_fullStr Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
title_full_unstemmed Electrohydrodynamic fabrication of core–shell PLGA nanoparticles with controlled release of cisplatin for enhanced cancer treatment
title_sort electrohydrodynamic fabrication of core–shell plga nanoparticles with controlled release of cisplatin for enhanced cancer treatment
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/c5577a17a8564e8d97fd258cd03393fe
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AT harkerah electrohydrodynamicfabricationofcorendashshellplgananoparticleswithcontrolledreleaseofcisplatinforenhancedcancertreatment
AT browningrj electrohydrodynamicfabricationofcorendashshellplgananoparticleswithcontrolledreleaseofcisplatinforenhancedcancertreatment
AT vassilevav electrohydrodynamicfabricationofcorendashshellplgananoparticleswithcontrolledreleaseofcisplatinforenhancedcancertreatment
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AT edirisinghem electrohydrodynamicfabricationofcorendashshellplgananoparticleswithcontrolledreleaseofcisplatinforenhancedcancertreatment
AT knowlesjc electrohydrodynamicfabricationofcorendashshellplgananoparticleswithcontrolledreleaseofcisplatinforenhancedcancertreatment
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