Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment

Summary: The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We coll...

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Autores principales:	Aminata Fofana, Mathilde Gendrin, Ottavia Romoli, G. Armel Bienvenu Yarbanga, Georges Anicet Ouédraogo, Rakiswende Serge Yerbanga, Jean-Bosco Ouédraogo
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Entomology Microbiome Science Q
Acceso en línea:	https://doaj.org/article/c565b193a9ae4a0ba1d5e924f2acf296
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spelling	oai:doaj.org-article:c565b193a9ae4a0ba1d5e924f2acf2962021-11-26T04:37:39ZAnalyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment2589-004210.1016/j.isci.2021.103416https://doaj.org/article/c565b193a9ae4a0ba1d5e924f2acf2962021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013870https://doaj.org/toc/2589-0042Summary: The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, Elizabethkingia, Wigglesworthia, Asaia, and Serratia. The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of Elizabethkingia and Asaia during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.Aminata FofanaMathilde GendrinOttavia RomoliG. Armel Bienvenu YarbangaGeorges Anicet OuédraogoRakiswende Serge YerbangaJean-Bosco OuédraogoElsevierarticleEntomologyMicrobiomeScienceQENiScience, Vol 24, Iss 12, Pp 103416- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Entomology Microbiome Science Q
spellingShingle	Entomology Microbiome Science Q Aminata Fofana Mathilde Gendrin Ottavia Romoli G. Armel Bienvenu Yarbanga Georges Anicet Ouédraogo Rakiswende Serge Yerbanga Jean-Bosco Ouédraogo Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
description	Summary: The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, Elizabethkingia, Wigglesworthia, Asaia, and Serratia. The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of Elizabethkingia and Asaia during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.
format	article
author	Aminata Fofana Mathilde Gendrin Ottavia Romoli G. Armel Bienvenu Yarbanga Georges Anicet Ouédraogo Rakiswende Serge Yerbanga Jean-Bosco Ouédraogo
author_facet	Aminata Fofana Mathilde Gendrin Ottavia Romoli G. Armel Bienvenu Yarbanga Georges Anicet Ouédraogo Rakiswende Serge Yerbanga Jean-Bosco Ouédraogo
author_sort	Aminata Fofana
title	Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
title_short	Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
title_full	Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
title_fullStr	Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
title_full_unstemmed	Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
title_sort	analyzing gut microbiota composition in individual anopheles mosquitoes after experimental treatment
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c565b193a9ae4a0ba1d5e924f2acf296
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Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment

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