Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics

Abstract The diagnosis of COVID-19 relies on the direct detection of SARS-CoV-2 RNA in respiratory specimens by RT-PCR. The pandemic spread of the disease caused an imbalance between demand and supply of materials and reagents needed for diagnostic purposes including swab sets. In a comparative effe...

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Autores principales:	Manfred Nairz, Rosa Bellmann-Weiler, Miriam Ladstätter, Falko Schüllner, Martina Zimmermann, Anna-Maria Koller, Silvia Blunder, Helene Naschberger, Werner Klotz, Manfred Herold, Sylvia Kerndler, Martina Jeske, David Haschka, Verena Petzer, Andrea Schroll, Thomas Sonnweber, Ivan Tancevski, Gernot Fritsche, Mariana E. G. de Araujo, Taras Stasyk, Lukas A. Huber, Andrea Griesmacher, Igor Theurl, Günter Weiss
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c56c694f023548f3a8774472903a47bc
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spelling	oai:doaj.org-article:c56c694f023548f3a8774472903a47bc2021-12-02T13:57:37ZOvercoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics10.1038/s41598-021-81782-82045-2322https://doaj.org/article/c56c694f023548f3a8774472903a47bc2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81782-8https://doaj.org/toc/2045-2322Abstract The diagnosis of COVID-19 relies on the direct detection of SARS-CoV-2 RNA in respiratory specimens by RT-PCR. The pandemic spread of the disease caused an imbalance between demand and supply of materials and reagents needed for diagnostic purposes including swab sets. In a comparative effectiveness study, we conducted serial follow-up swabs in hospitalized laboratory-confirmed COVID-19 patients. We assessed the diagnostic performance of an in-house system developed according to recommendations by the US CDC. In a total of 96 serial swabs, we found significant differences in the accuracy of the different swab systems to generate a positive result in SARS-CoV-2 RT-PCR, ranging from around 50 to 80%. Of note, an in-house swab system was superior to most commercially available sets as reflected by significantly lower Ct values of viral genes. Thus, a simple combination of broadly available materials may enable diagnostic laboratories to bypass global limitations in the supply of swab sets.Manfred NairzRosa Bellmann-WeilerMiriam LadstätterFalko SchüllnerMartina ZimmermannAnna-Maria KollerSilvia BlunderHelene NaschbergerWerner KlotzManfred HeroldSylvia KerndlerMartina JeskeDavid HaschkaVerena PetzerAndrea SchrollThomas SonnweberIvan TancevskiGernot FritscheMariana E. G. de AraujoTaras StasykLukas A. HuberAndrea GriesmacherIgor TheurlGünter WeissNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manfred Nairz Rosa Bellmann-Weiler Miriam Ladstätter Falko Schüllner Martina Zimmermann Anna-Maria Koller Silvia Blunder Helene Naschberger Werner Klotz Manfred Herold Sylvia Kerndler Martina Jeske David Haschka Verena Petzer Andrea Schroll Thomas Sonnweber Ivan Tancevski Gernot Fritsche Mariana E. G. de Araujo Taras Stasyk Lukas A. Huber Andrea Griesmacher Igor Theurl Günter Weiss Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
description	Abstract The diagnosis of COVID-19 relies on the direct detection of SARS-CoV-2 RNA in respiratory specimens by RT-PCR. The pandemic spread of the disease caused an imbalance between demand and supply of materials and reagents needed for diagnostic purposes including swab sets. In a comparative effectiveness study, we conducted serial follow-up swabs in hospitalized laboratory-confirmed COVID-19 patients. We assessed the diagnostic performance of an in-house system developed according to recommendations by the US CDC. In a total of 96 serial swabs, we found significant differences in the accuracy of the different swab systems to generate a positive result in SARS-CoV-2 RT-PCR, ranging from around 50 to 80%. Of note, an in-house swab system was superior to most commercially available sets as reflected by significantly lower Ct values of viral genes. Thus, a simple combination of broadly available materials may enable diagnostic laboratories to bypass global limitations in the supply of swab sets.
format	article
author	Manfred Nairz Rosa Bellmann-Weiler Miriam Ladstätter Falko Schüllner Martina Zimmermann Anna-Maria Koller Silvia Blunder Helene Naschberger Werner Klotz Manfred Herold Sylvia Kerndler Martina Jeske David Haschka Verena Petzer Andrea Schroll Thomas Sonnweber Ivan Tancevski Gernot Fritsche Mariana E. G. de Araujo Taras Stasyk Lukas A. Huber Andrea Griesmacher Igor Theurl Günter Weiss
author_facet	Manfred Nairz Rosa Bellmann-Weiler Miriam Ladstätter Falko Schüllner Martina Zimmermann Anna-Maria Koller Silvia Blunder Helene Naschberger Werner Klotz Manfred Herold Sylvia Kerndler Martina Jeske David Haschka Verena Petzer Andrea Schroll Thomas Sonnweber Ivan Tancevski Gernot Fritsche Mariana E. G. de Araujo Taras Stasyk Lukas A. Huber Andrea Griesmacher Igor Theurl Günter Weiss
author_sort	Manfred Nairz
title	Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
title_short	Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
title_full	Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
title_fullStr	Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
title_full_unstemmed	Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics
title_sort	overcoming limitations in the availability of swabs systems used for sars-cov-2 laboratory diagnostics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c56c694f023548f3a8774472903a47bc
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Overcoming limitations in the availability of swabs systems used for SARS-CoV-2 laboratory diagnostics

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