Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection

Quan-Quan Zhuang,1 Zhi-Hang Lin,1 Yan-Cheng Jiang,1 Hao-Hua Deng,2 Shao-Bin He,1,3 Li-Ting Su,4 Xiao-Qiong Shi,2 Wei Chen2 1Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 2Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical U...

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Autores principales:	Zhuang QQ, Lin ZH, Jiang YC, Deng HH, He SB, Su LT, Shi XQ, Chen W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Enzyme mimics cobalt selenide peroxidase-like activity uric acid human serum Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c5704d2a0ac347fc8fa215e346c946ea
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spelling	oai:doaj.org-article:c5704d2a0ac347fc8fa215e346c946ea2021-12-02T05:10:46ZPeroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection1178-2013https://doaj.org/article/c5704d2a0ac347fc8fa215e346c946ea2017-04-01T00:00:00Zhttps://www.dovepress.com/peroxidase-like-activity-of-nanocrystalline-cobalt-selenide-and-its-ap-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Quan-Quan Zhuang,1 Zhi-Hang Lin,1 Yan-Cheng Jiang,1 Hao-Hua Deng,2 Shao-Bin He,1,3 Li-Ting Su,4 Xiao-Qiong Shi,2 Wei Chen2 1Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 2Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 3Department of Pharmacy, Quanzhou Infectious Disease Hospital, 4Department of Pharmaceutical Analysis, Quanzhou Medical College, Quanzhou, People’s Republic of China Abstract: Dendrite-like cobalt selenide nanostructures were synthesized from cobalt and selenium powder precursors by a solvothermal method in anhydrous ethylenediamine. The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed based on the nanocrystalline cobalt selenide-catalyzed coupling reaction between N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and 4-aminoantipyrine (4-AAP) in the presence of H2O2. Under optimum conditions, the absorbance was proportional to the concentration of UA over the range of 2.0–40 µM with a detection limit of 0.5 µM. The applicability of the proposed method has been validated by determination of UA in human serum samples with satisfactory results. Keywords: enzyme mimics, cobalt selenide, peroxidase-like activity, uric acid, human serumZhuang QQLin ZHJiang YCDeng HHHe SBSu LTShi XQChen WDove Medical PressarticleEnzyme mimicscobalt selenideperoxidase-like activityuric acidhuman serumMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3295-3302 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Enzyme mimics cobalt selenide peroxidase-like activity uric acid human serum Medicine (General) R5-920
spellingShingle	Enzyme mimics cobalt selenide peroxidase-like activity uric acid human serum Medicine (General) R5-920 Zhuang QQ Lin ZH Jiang YC Deng HH He SB Su LT Shi XQ Chen W Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
description	Quan-Quan Zhuang,1 Zhi-Hang Lin,1 Yan-Cheng Jiang,1 Hao-Hua Deng,2 Shao-Bin He,1,3 Li-Ting Su,4 Xiao-Qiong Shi,2 Wei Chen2 1Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou, 2Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 3Department of Pharmacy, Quanzhou Infectious Disease Hospital, 4Department of Pharmaceutical Analysis, Quanzhou Medical College, Quanzhou, People’s Republic of China Abstract: Dendrite-like cobalt selenide nanostructures were synthesized from cobalt and selenium powder precursors by a solvothermal method in anhydrous ethylenediamine. The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed based on the nanocrystalline cobalt selenide-catalyzed coupling reaction between N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and 4-aminoantipyrine (4-AAP) in the presence of H2O2. Under optimum conditions, the absorbance was proportional to the concentration of UA over the range of 2.0–40 µM with a detection limit of 0.5 µM. The applicability of the proposed method has been validated by determination of UA in human serum samples with satisfactory results. Keywords: enzyme mimics, cobalt selenide, peroxidase-like activity, uric acid, human serum
format	article
author	Zhuang QQ Lin ZH Jiang YC Deng HH He SB Su LT Shi XQ Chen W
author_facet	Zhuang QQ Lin ZH Jiang YC Deng HH He SB Su LT Shi XQ Chen W
author_sort	Zhuang QQ
title	Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
title_short	Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
title_full	Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
title_fullStr	Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
title_full_unstemmed	Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
title_sort	peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/c5704d2a0ac347fc8fa215e346c946ea
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Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection

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