Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome

Emerging evidence demonstrates a connection between microbiome composition and suboptimal response to vaccines (vaccine hyporesponse). Harnessing the interaction between microbes and the immune system could provide novel therapeutic strategies for improving vaccine response. Currently we do not full...

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Autores principales:	Gokul Swaminathan, Michael Citron, Jianying Xiao, James E. Norton, Abigail L. Reens, Begüm D. Topçuoğlu, Julia M. Maritz, Keun-Joong Lee, Daniel C. Freed, Teresa M. Weber, Cory H. White, Mahika Kadam, Erin Spofford, Erin Bryant-Hall, Gino Salituro, Sushma Kommineni, Xue Liang, Olga Danilchanka, Jane A. Fontenot, Christopher H. Woelk, Dario A. Gutierrez, Daria J. Hazuda, Geoffrey D. Hannigan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microbiome vaccines immunity Medicine R
Acceso en línea:	https://doaj.org/article/c57141bfb4b545c0b73222a00565d3e1
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spelling	oai:doaj.org-article:c57141bfb4b545c0b73222a00565d3e12021-11-25T19:11:27ZVaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome10.3390/vaccines91113402076-393Xhttps://doaj.org/article/c57141bfb4b545c0b73222a00565d3e12021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1340https://doaj.org/toc/2076-393XEmerging evidence demonstrates a connection between microbiome composition and suboptimal response to vaccines (vaccine hyporesponse). Harnessing the interaction between microbes and the immune system could provide novel therapeutic strategies for improving vaccine response. Currently we do not fully understand the mechanisms and dynamics by which the microbiome influences vaccine response. Using both mouse and non-human primate models, we report that short-term oral treatment with a single antibiotic (vancomycin) results in the disruption of the gut microbiome and this correlates with a decrease in systemic levels of antigen-specific IgG upon subsequent parenteral vaccination. We further show that recovery of microbial diversity before vaccination prevents antibiotic-induced vaccine hyporesponse, and that the antigen specific IgG response correlates with the recovery of microbiome diversity. RNA sequencing analysis of small intestine, spleen, whole blood, and secondary lymphoid organs from antibiotic treated mice revealed a dramatic impact on the immune system, and a muted inflammatory signature is correlated with loss of bacteria from <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, and <i>Clostridiaceae</i>. These results suggest that microbially modulated immune pathways may be leveraged to promote vaccine response and will inform future vaccine design and development strategies.Gokul SwaminathanMichael CitronJianying XiaoJames E. NortonAbigail L. ReensBegüm D. TopçuoğluJulia M. MaritzKeun-Joong LeeDaniel C. FreedTeresa M. WeberCory H. WhiteMahika KadamErin SpoffordErin Bryant-HallGino SalituroSushma KommineniXue LiangOlga DanilchankaJane A. FontenotChristopher H. WoelkDario A. GutierrezDaria J. HazudaGeoffrey D. HanniganMDPI AGarticlemicrobiomevaccinesimmunityMedicineRENVaccines, Vol 9, Iss 1340, p 1340 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiome vaccines immunity Medicine R
spellingShingle	microbiome vaccines immunity Medicine R Gokul Swaminathan Michael Citron Jianying Xiao James E. Norton Abigail L. Reens Begüm D. Topçuoğlu Julia M. Maritz Keun-Joong Lee Daniel C. Freed Teresa M. Weber Cory H. White Mahika Kadam Erin Spofford Erin Bryant-Hall Gino Salituro Sushma Kommineni Xue Liang Olga Danilchanka Jane A. Fontenot Christopher H. Woelk Dario A. Gutierrez Daria J. Hazuda Geoffrey D. Hannigan Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
description	Emerging evidence demonstrates a connection between microbiome composition and suboptimal response to vaccines (vaccine hyporesponse). Harnessing the interaction between microbes and the immune system could provide novel therapeutic strategies for improving vaccine response. Currently we do not fully understand the mechanisms and dynamics by which the microbiome influences vaccine response. Using both mouse and non-human primate models, we report that short-term oral treatment with a single antibiotic (vancomycin) results in the disruption of the gut microbiome and this correlates with a decrease in systemic levels of antigen-specific IgG upon subsequent parenteral vaccination. We further show that recovery of microbial diversity before vaccination prevents antibiotic-induced vaccine hyporesponse, and that the antigen specific IgG response correlates with the recovery of microbiome diversity. RNA sequencing analysis of small intestine, spleen, whole blood, and secondary lymphoid organs from antibiotic treated mice revealed a dramatic impact on the immune system, and a muted inflammatory signature is correlated with loss of bacteria from <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, and <i>Clostridiaceae</i>. These results suggest that microbially modulated immune pathways may be leveraged to promote vaccine response and will inform future vaccine design and development strategies.
format	article
author	Gokul Swaminathan Michael Citron Jianying Xiao James E. Norton Abigail L. Reens Begüm D. Topçuoğlu Julia M. Maritz Keun-Joong Lee Daniel C. Freed Teresa M. Weber Cory H. White Mahika Kadam Erin Spofford Erin Bryant-Hall Gino Salituro Sushma Kommineni Xue Liang Olga Danilchanka Jane A. Fontenot Christopher H. Woelk Dario A. Gutierrez Daria J. Hazuda Geoffrey D. Hannigan
author_facet	Gokul Swaminathan Michael Citron Jianying Xiao James E. Norton Abigail L. Reens Begüm D. Topçuoğlu Julia M. Maritz Keun-Joong Lee Daniel C. Freed Teresa M. Weber Cory H. White Mahika Kadam Erin Spofford Erin Bryant-Hall Gino Salituro Sushma Kommineni Xue Liang Olga Danilchanka Jane A. Fontenot Christopher H. Woelk Dario A. Gutierrez Daria J. Hazuda Geoffrey D. Hannigan
author_sort	Gokul Swaminathan
title	Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
title_short	Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
title_full	Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
title_fullStr	Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
title_full_unstemmed	Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome
title_sort	vaccine hyporesponse induced by individual antibiotic treatment in mice and non-human primates is diminished upon recovery of the gut microbiome
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c57141bfb4b545c0b73222a00565d3e1
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Vaccine Hyporesponse Induced by Individual Antibiotic Treatment in Mice and Non-Human Primates Is Diminished upon Recovery of the Gut Microbiome

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