Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection

The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. Bacillus spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rafaela A. Santos, Marta Monteiro, Fábio Rangel, Russell Jerusik, Maria J. Saavedra, António Paulo Carvalho, Aires Oliva-Teles, Cláudia R. Serra
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fish diseases quorum-quenching <i>Bacillus</i> spp. zebrafish larvae Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c5720ecb8e3f4d65a41f3c89c84449cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c5720ecb8e3f4d65a41f3c89c84449cb
record_format	dspace
spelling	oai:doaj.org-article:c5720ecb8e3f4d65a41f3c89c84449cb2021-11-25T18:12:44Z<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection10.3390/md191106021660-3397https://doaj.org/article/c5720ecb8e3f4d65a41f3c89c84449cb2021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/602https://doaj.org/toc/1660-3397The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. <i>Bacillus</i> spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 <i>Bacillus</i> spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested <i>Bacillus</i> spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely <i>Aeromonas</i> spp., <i>Vibrio</i> spp., <i>Photobacterium damselae</i>, <i>Edwardsiela tarda</i>, and <i>Shigella sonnei</i>. The results revealed that <i>A. veronii</i> and <i>E. tarda</i> produce QS molecules that are detectable by the <i>Chr. violaceum</i> biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as <i>B. subtilis</i>, <i>B. vezelensis</i>, and <i>B. pumilus</i>, significantly reduced the pathogenicity of <i>E. tarda</i> in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three <i>Bacillus</i> spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.Rafaela A. SantosMarta MonteiroFábio RangelRussell JerusikMaria J. SaavedraAntónio Paulo CarvalhoAires Oliva-TelesCláudia R. SerraMDPI AGarticlefish diseasesquorum-quenching<i>Bacillus</i> spp.zebrafish larvaeBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 602, p 602 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fish diseases quorum-quenching <i>Bacillus</i> spp. zebrafish larvae Biology (General) QH301-705.5
spellingShingle	fish diseases quorum-quenching <i>Bacillus</i> spp. zebrafish larvae Biology (General) QH301-705.5 Rafaela A. Santos Marta Monteiro Fábio Rangel Russell Jerusik Maria J. Saavedra António Paulo Carvalho Aires Oliva-Teles Cláudia R. Serra <i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
description	The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. <i>Bacillus</i> spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 <i>Bacillus</i> spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested <i>Bacillus</i> spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely <i>Aeromonas</i> spp., <i>Vibrio</i> spp., <i>Photobacterium damselae</i>, <i>Edwardsiela tarda</i>, and <i>Shigella sonnei</i>. The results revealed that <i>A. veronii</i> and <i>E. tarda</i> produce QS molecules that are detectable by the <i>Chr. violaceum</i> biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as <i>B. subtilis</i>, <i>B. vezelensis</i>, and <i>B. pumilus</i>, significantly reduced the pathogenicity of <i>E. tarda</i> in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three <i>Bacillus</i> spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.
format	article
author	Rafaela A. Santos Marta Monteiro Fábio Rangel Russell Jerusik Maria J. Saavedra António Paulo Carvalho Aires Oliva-Teles Cláudia R. Serra
author_facet	Rafaela A. Santos Marta Monteiro Fábio Rangel Russell Jerusik Maria J. Saavedra António Paulo Carvalho Aires Oliva-Teles Cláudia R. Serra
author_sort	Rafaela A. Santos
title	<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
title_short	<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
title_full	<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
title_fullStr	<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
title_full_unstemmed	<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection
title_sort	<i>bacillus</i> spp. inhibit <i>edwardsiella tarda</i> quorum-sensing and fish infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c5720ecb8e3f4d65a41f3c89c84449cb
work_keys_str_mv	AT rafaelaasantos ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT martamonteiro ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT fabiorangel ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT russelljerusik ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT mariajsaavedra ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT antoniopaulocarvalho ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT airesolivateles ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection AT claudiarserra ibacillusisppinhibitiedwardsiellatardaiquorumsensingandfishinfection
_version_	1718411522308308992

<i>Bacillus</i> spp. Inhibit <i>Edwardsiella tarda</i> Quorum-Sensing and Fish Infection

Ejemplares similares