Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection

Abstract The implementation of RNAi technology into the clinical practice has been significantly postponing due to the issues regarding to the delivery of naked siRNA predominantly to target cells. Here we report the approach to enhance the efficiency of siRNA delivery by encapsulating the siRNA int...

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Autores principales:	Alexander S. Timin, Albert R. Muslimov, Aleksandra V. Petrova, Kirill V. Lepik, Maria V. Okilova, Andrey V. Vasin, Boris V. Afanasyev, Gleb B. Sukhorukov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c572e8c26bc846c0ab0665b989070caf
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spelling	oai:doaj.org-article:c572e8c26bc846c0ab0665b989070caf2021-12-02T12:30:44ZHybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection10.1038/s41598-017-00200-02045-2322https://doaj.org/article/c572e8c26bc846c0ab0665b989070caf2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00200-0https://doaj.org/toc/2045-2322Abstract The implementation of RNAi technology into the clinical practice has been significantly postponing due to the issues regarding to the delivery of naked siRNA predominantly to target cells. Here we report the approach to enhance the efficiency of siRNA delivery by encapsulating the siRNA into new carrier systems which are obtained via the combination of widely used layer-by-layer technique and in situ modification by sol-gel chemistry. We used three types of siRNAs (NP-717, NP-1155 and NP-1496) in encapsulated form as new therapeutic agents against H1N1 influenza virus infection. By employing the hybrid microcontainers for the siRNA encapsulation we demonstrate the reduction of viral nucleoprotein (NP) level and inhibition of influenza virus production in infected cell lines (MDCK and A549). The obtained hybrid carriers based on assembled biodegradable polyelectrolytes and sol-gel coating possess several advantages such as a high cell uptake efficiency, low toxicity, efficient intracellular delivery of siRNAs and the protection of siRNAs from premature degradation before reaching the target cells. These findings underpin a great potential of versatile microencapsulation technology for the development of anti-viral RNAi delivery systems against influenza virus infection.Alexander S. TiminAlbert R. MuslimovAleksandra V. PetrovaKirill V. LepikMaria V. OkilovaAndrey V. VasinBoris V. AfanasyevGleb B. SukhorukovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander S. Timin Albert R. Muslimov Aleksandra V. Petrova Kirill V. Lepik Maria V. Okilova Andrey V. Vasin Boris V. Afanasyev Gleb B. Sukhorukov Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
description	Abstract The implementation of RNAi technology into the clinical practice has been significantly postponing due to the issues regarding to the delivery of naked siRNA predominantly to target cells. Here we report the approach to enhance the efficiency of siRNA delivery by encapsulating the siRNA into new carrier systems which are obtained via the combination of widely used layer-by-layer technique and in situ modification by sol-gel chemistry. We used three types of siRNAs (NP-717, NP-1155 and NP-1496) in encapsulated form as new therapeutic agents against H1N1 influenza virus infection. By employing the hybrid microcontainers for the siRNA encapsulation we demonstrate the reduction of viral nucleoprotein (NP) level and inhibition of influenza virus production in infected cell lines (MDCK and A549). The obtained hybrid carriers based on assembled biodegradable polyelectrolytes and sol-gel coating possess several advantages such as a high cell uptake efficiency, low toxicity, efficient intracellular delivery of siRNAs and the protection of siRNAs from premature degradation before reaching the target cells. These findings underpin a great potential of versatile microencapsulation technology for the development of anti-viral RNAi delivery systems against influenza virus infection.
format	article
author	Alexander S. Timin Albert R. Muslimov Aleksandra V. Petrova Kirill V. Lepik Maria V. Okilova Andrey V. Vasin Boris V. Afanasyev Gleb B. Sukhorukov
author_facet	Alexander S. Timin Albert R. Muslimov Aleksandra V. Petrova Kirill V. Lepik Maria V. Okilova Andrey V. Vasin Boris V. Afanasyev Gleb B. Sukhorukov
author_sort	Alexander S. Timin
title	Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
title_short	Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
title_full	Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
title_fullStr	Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
title_full_unstemmed	Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection
title_sort	hybrid inorganic-organic capsules for efficient intracellular delivery of novel sirnas against influenza a (h1n1) virus infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c572e8c26bc846c0ab0665b989070caf
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Hybrid inorganic-organic capsules for efficient intracellular delivery of novel siRNAs against influenza A (H1N1) virus infection

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