Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

Background and Aim: Mesenchymal stem cells (MSCs) transplanted into the testes of rats with testicular failure can help rescue fertility. However, the low viability of transplanted MSCs limits the success of this treatment. This study aimed to determine the effectiveness of MSCs cultured under hypox...

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Autores principales:	Erma Safitri, Hery Purnobasuki
Formato:	article
Lenguaje:	EN
Publicado:	Veterinary World 2021
Materias:	fertility hypoxia culture malnutrition stem cell testicular failure Animal culture SF1-1100 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/c578cd8cfd1c4bd792d87a19e356e9f5
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spelling	oai:doaj.org-article:c578cd8cfd1c4bd792d87a19e356e9f52021-11-30T07:30:04ZEffectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)10.14202/vetworld.2021.3056-30640972-89882231-0916https://doaj.org/article/c578cd8cfd1c4bd792d87a19e356e9f52021-11-01T00:00:00Zhttp://www.veterinaryworld.org/Vol.14/November-2021/28.pdfhttps://doaj.org/toc/0972-8988https://doaj.org/toc/2231-0916Background and Aim: Mesenchymal stem cells (MSCs) transplanted into the testes of rats with testicular failure can help rescue fertility. However, the low viability of transplanted MSCs limits the success of this treatment. This study aimed to determine the effectiveness of MSCs cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus). Materials and Methods: Bone marrow-derived MSCs (200 million cells/rat) were transplanted into male rat models with induced infertility (10 rats/treatment group) after 4 days of culture in 21% O2 (normoxia) and 1% O2 (hypoxia). Ten fertile and 10 untreated infertile rats served as controls. In the infertile male rats that had been fasted from food for 5 days, the fasting condition induced malnutrition and then resulted in testicular failure. Results: The results indicated that the MSCs cultured under hypoxic conditions were more effective than those cultured in normoxic conditions as a treatment for testicular failure in infertile male rats based on the increased number of cells expressing p63 as a quiescent cell marker and ETV5 as a transcription factor expressed in Sertoli and germ cells. Furthermore, the structure of the seminiferous tubules, which contain spermatogonia, primary and secondary spermatocytes, and spermatid, Sertoli, and Leydig cells, was improved in infertile male rats treated with the MSCs cultured under hypoxic conditions. Conclusion: The testicular transplantation of MSCs cultured under hypoxic conditions was an effective treatment for testicular failure in rats.Erma SafitriHery PurnobasukiVeterinary Worldarticlefertilityhypoxia culturemalnutritionstem celltesticular failureAnimal cultureSF1-1100Veterinary medicineSF600-1100ENVeterinary World, Vol 14, Iss 11, Pp 3056-3064 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fertility hypoxia culture malnutrition stem cell testicular failure Animal culture SF1-1100 Veterinary medicine SF600-1100
spellingShingle	fertility hypoxia culture malnutrition stem cell testicular failure Animal culture SF1-1100 Veterinary medicine SF600-1100 Erma Safitri Hery Purnobasuki Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
description	Background and Aim: Mesenchymal stem cells (MSCs) transplanted into the testes of rats with testicular failure can help rescue fertility. However, the low viability of transplanted MSCs limits the success of this treatment. This study aimed to determine the effectiveness of MSCs cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus). Materials and Methods: Bone marrow-derived MSCs (200 million cells/rat) were transplanted into male rat models with induced infertility (10 rats/treatment group) after 4 days of culture in 21% O2 (normoxia) and 1% O2 (hypoxia). Ten fertile and 10 untreated infertile rats served as controls. In the infertile male rats that had been fasted from food for 5 days, the fasting condition induced malnutrition and then resulted in testicular failure. Results: The results indicated that the MSCs cultured under hypoxic conditions were more effective than those cultured in normoxic conditions as a treatment for testicular failure in infertile male rats based on the increased number of cells expressing p63 as a quiescent cell marker and ETV5 as a transcription factor expressed in Sertoli and germ cells. Furthermore, the structure of the seminiferous tubules, which contain spermatogonia, primary and secondary spermatocytes, and spermatid, Sertoli, and Leydig cells, was improved in infertile male rats treated with the MSCs cultured under hypoxic conditions. Conclusion: The testicular transplantation of MSCs cultured under hypoxic conditions was an effective treatment for testicular failure in rats.
format	article
author	Erma Safitri Hery Purnobasuki
author_facet	Erma Safitri Hery Purnobasuki
author_sort	Erma Safitri
title	Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
title_short	Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
title_full	Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
title_fullStr	Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
title_full_unstemmed	Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)
title_sort	effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (rattus norvegicus)
publisher	Veterinary World
publishDate	2021
url	https://doaj.org/article/c578cd8cfd1c4bd792d87a19e356e9f5
work_keys_str_mv	AT ermasafitri effectivenessofmesenchymalstemcellsculturedunderhypoxiatoincreasethefertilityrateinratsrattusnorvegicus AT herypurnobasuki effectivenessofmesenchymalstemcellsculturedunderhypoxiatoincreasethefertilityrateinratsrattusnorvegicus
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Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

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