Functional neuronal cells generated by human parthenogenetic stem cells.

Parent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. We determined the potential of hp...

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Autores principales:	Ruhel Ahmad, Wanja Wolber, Sigrid Eckardt, Philipp Koch, Jessica Schmitt, Ruslan Semechkin, Christian Geis, Manfred Heckmann, Oliver Brüstle, John K McLaughlin, Anna-Leena Sirén, Albrecht M Müller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c57bc4aeedc64ee5a7df3b27f23bb6a2
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spelling	oai:doaj.org-article:c57bc4aeedc64ee5a7df3b27f23bb6a22021-11-18T07:09:31ZFunctional neuronal cells generated by human parthenogenetic stem cells.1932-620310.1371/journal.pone.0042800https://doaj.org/article/c57bc4aeedc64ee5a7df3b27f23bb6a22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880113/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Parent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. We determined the potential of hpESCs to differentiate into various neural subtypes. Concurrently, we examined DNA methylation and expression status of imprinted genes. Under culture conditions promoting neural differentiation, hpESC-derived neural stem cells (hpNSCs) gave rise to glia and neuron-like cells that expressed subtype-specific markers and generated action potentials. Analysis of imprinting in hpESCs and in hpNSCs revealed that maternal-specific gene expression patterns and imprinting marks were generally maintained in PG cells upon differentiation. Our results demonstrate that despite the lack of a paternal genome, hpESCs generate proliferating NSCs that are capable of differentiation into physiologically functional neuron-like cells and maintain allele-specific expression of imprinted genes. Thus, hpESCs can serve as a model to study the role of maternal and paternal genomes in neural development and to better understand imprinting-associated brain diseases.Ruhel AhmadWanja WolberSigrid EckardtPhilipp KochJessica SchmittRuslan SemechkinChristian GeisManfred HeckmannOliver BrüstleJohn K McLaughlinAnna-Leena SirénAlbrecht M MüllerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42800 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruhel Ahmad Wanja Wolber Sigrid Eckardt Philipp Koch Jessica Schmitt Ruslan Semechkin Christian Geis Manfred Heckmann Oliver Brüstle John K McLaughlin Anna-Leena Sirén Albrecht M Müller Functional neuronal cells generated by human parthenogenetic stem cells.
description	Parent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. We determined the potential of hpESCs to differentiate into various neural subtypes. Concurrently, we examined DNA methylation and expression status of imprinted genes. Under culture conditions promoting neural differentiation, hpESC-derived neural stem cells (hpNSCs) gave rise to glia and neuron-like cells that expressed subtype-specific markers and generated action potentials. Analysis of imprinting in hpESCs and in hpNSCs revealed that maternal-specific gene expression patterns and imprinting marks were generally maintained in PG cells upon differentiation. Our results demonstrate that despite the lack of a paternal genome, hpESCs generate proliferating NSCs that are capable of differentiation into physiologically functional neuron-like cells and maintain allele-specific expression of imprinted genes. Thus, hpESCs can serve as a model to study the role of maternal and paternal genomes in neural development and to better understand imprinting-associated brain diseases.
format	article
author	Ruhel Ahmad Wanja Wolber Sigrid Eckardt Philipp Koch Jessica Schmitt Ruslan Semechkin Christian Geis Manfred Heckmann Oliver Brüstle John K McLaughlin Anna-Leena Sirén Albrecht M Müller
author_facet	Ruhel Ahmad Wanja Wolber Sigrid Eckardt Philipp Koch Jessica Schmitt Ruslan Semechkin Christian Geis Manfred Heckmann Oliver Brüstle John K McLaughlin Anna-Leena Sirén Albrecht M Müller
author_sort	Ruhel Ahmad
title	Functional neuronal cells generated by human parthenogenetic stem cells.
title_short	Functional neuronal cells generated by human parthenogenetic stem cells.
title_full	Functional neuronal cells generated by human parthenogenetic stem cells.
title_fullStr	Functional neuronal cells generated by human parthenogenetic stem cells.
title_full_unstemmed	Functional neuronal cells generated by human parthenogenetic stem cells.
title_sort	functional neuronal cells generated by human parthenogenetic stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c57bc4aeedc64ee5a7df3b27f23bb6a2
work_keys_str_mv	AT ruhelahmad functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT wanjawolber functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT sigrideckardt functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT philippkoch functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT jessicaschmitt functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT ruslansemechkin functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT christiangeis functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT manfredheckmann functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT oliverbrustle functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT johnkmclaughlin functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT annaleenasiren functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells AT albrechtmmuller functionalneuronalcellsgeneratedbyhumanparthenogeneticstemcells
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Functional neuronal cells generated by human parthenogenetic stem cells.

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