Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Roman Generalov1,2, Simona Kavaliauskiene1, Sara Westr&oslash;m1, Wei Chen3, Solveig Kristensen2, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway; 2School of Pharmacy, University of Oslo, Oslo,...

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Autores principales:	Generalov R, Kavaliauskiene S, Westr&oslash;m S, Chen W, Kristensen S, Juzenas P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c589943a21f7444c82c173476e02b5ba
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spelling	oai:doaj.org-article:c589943a21f7444c82c173476e02b5ba2021-12-02T01:11:24ZEntrapment in phospholipid vesicles quenches photoactivity of quantum dots1176-91141178-2013https://doaj.org/article/c589943a21f7444c82c173476e02b5ba2011-09-01T00:00:00Zhttp://www.dovepress.com/entrapment-in-phospholipid-vesicles-quenches-photoactivity-of-quantum--a8226https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Roman Generalov1,2, Simona Kavaliauskiene1, Sara Westr&oslash;m1, Wei Chen3, Solveig Kristensen2, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway; 2School of Pharmacy, University of Oslo, Oslo, Norway; 3Department of Physics, The University of Texas at Arlington, Arlington, TX, USAAbstract: Quantum dots have emerged with great promise for biological applications as fluorescent markers for immunostaining, labels for intracellular trafficking, and photosensitizers for photodynamic therapy. However, upon entry into a cell, quantum dots are trapped and their fluorescence is quenched in endocytic vesicles such as endosomes and lysosomes. In this study, the photophysical properties of quantum dots were investigated in liposomes as an in vitro vesicle model. Entrapment of quantum dots in liposomes decreases their fluorescence lifetime and intensity. Generation of free radicals by liposomal quantum dots is inhibited compared to that of free quantum dots. Nevertheless, quantum dot fluorescence lifetime and intensity increases due to photolysis of liposomes during irradiation. In addition, protein adsorption on the quantum dot surface and the acidic environment of vesicles also lead to quenching of quantum dot fluorescence, which reappears during irradiation. In conclusion, the in vitro model of phospholipid vesicles has demonstrated that those quantum dots that are fated to be entrapped in endocytic vesicles lose their fluorescence and ability to act as photosensitizers.Keywords: fluorescence lifetime, free radicals, liposomes, lipodots, reactive oxygen speciesGeneralov RKavaliauskiene SWestr&oslash;m SChen WKristensen SJuzenas PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1875-1888 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Generalov R Kavaliauskiene S Westr&oslash;m S Chen W Kristensen S Juzenas P Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
description	Roman Generalov1,2, Simona Kavaliauskiene1, Sara Westr&oslash;m1, Wei Chen3, Solveig Kristensen2, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway; 2School of Pharmacy, University of Oslo, Oslo, Norway; 3Department of Physics, The University of Texas at Arlington, Arlington, TX, USAAbstract: Quantum dots have emerged with great promise for biological applications as fluorescent markers for immunostaining, labels for intracellular trafficking, and photosensitizers for photodynamic therapy. However, upon entry into a cell, quantum dots are trapped and their fluorescence is quenched in endocytic vesicles such as endosomes and lysosomes. In this study, the photophysical properties of quantum dots were investigated in liposomes as an in vitro vesicle model. Entrapment of quantum dots in liposomes decreases their fluorescence lifetime and intensity. Generation of free radicals by liposomal quantum dots is inhibited compared to that of free quantum dots. Nevertheless, quantum dot fluorescence lifetime and intensity increases due to photolysis of liposomes during irradiation. In addition, protein adsorption on the quantum dot surface and the acidic environment of vesicles also lead to quenching of quantum dot fluorescence, which reappears during irradiation. In conclusion, the in vitro model of phospholipid vesicles has demonstrated that those quantum dots that are fated to be entrapped in endocytic vesicles lose their fluorescence and ability to act as photosensitizers.Keywords: fluorescence lifetime, free radicals, liposomes, lipodots, reactive oxygen species
format	article
author	Generalov R Kavaliauskiene S Westr&oslash;m S Chen W Kristensen S Juzenas P
author_facet	Generalov R Kavaliauskiene S Westr&oslash;m S Chen W Kristensen S Juzenas P
author_sort	Generalov R
title	Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
title_short	Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
title_full	Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
title_fullStr	Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
title_full_unstemmed	Entrapment in phospholipid vesicles quenches photoactivity of quantum dots
title_sort	entrapment in phospholipid vesicles quenches photoactivity of quantum dots
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/c589943a21f7444c82c173476e02b5ba
work_keys_str_mv	AT generalovr entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots AT kavaliauskienes entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots AT westramposlashms entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots AT chenw entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots AT kristensens entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots AT juzenasp entrapmentinphospholipidvesiclesquenchesphotoactivityofquantumdots
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Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

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