Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors

Abstract Gaining an in-depth understanding of the response of Saccharomyces cerevisiae to the different inhibitors generated during the pretreatment of lignocellulosic material is driving the development of new strains with higher inhibitor tolerances. The objective of this study is to assess, using...

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Autores principales: Pau Cabaneros López, Chuantao Peng, Nils Arneborg, Helena Junicke, Krist V. Gernaey
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/c58e22cfc8cc4f4e973391a7e709ac3e
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spelling oai:doaj.org-article:c58e22cfc8cc4f4e973391a7e709ac3e2021-12-02T17:04:36ZAnalysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors10.1038/s41598-021-86135-z2045-2322https://doaj.org/article/c58e22cfc8cc4f4e973391a7e709ac3e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86135-zhttps://doaj.org/toc/2045-2322Abstract Gaining an in-depth understanding of the response of Saccharomyces cerevisiae to the different inhibitors generated during the pretreatment of lignocellulosic material is driving the development of new strains with higher inhibitor tolerances. The objective of this study is to assess, using flow cytometry, how three common inhibitors (vanillin, furfural, and acetic acid) affect the membrane potential, the membrane permeability and the concentration of reactive oxygen species (ROS) during the different fermentations. The membrane potential decreased during the detoxification phase and reflected on the different mechanisms of the toxicity of the inhibitors. While vanillin and furfural caused a metabolic inhibition and a gradual depolarization, acetic acid toxicity was related to fast acidification of the cytosol, causing an immediate depolarization. In the absence of acetic acid, ethanol increased membrane permeability, indicating a possible acquired tolerance to ethanol due to an adaptive response to acetic acid. The intracellular ROS concentration also increased in the presence of the inhibitors, indicating oxidative stress. Measuring these features with flow cytometry allows a real-time assessment of the stress of a cell culture, which can be used in the development of new yeast strains and to design new propagation strategies to pre-adapt the cell cultures to the inhibitors.Pau Cabaneros LópezChuantao PengNils ArneborgHelena JunickeKrist V. GernaeyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Pau Cabaneros López
Chuantao Peng
Nils Arneborg
Helena Junicke
Krist V. Gernaey
Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
description Abstract Gaining an in-depth understanding of the response of Saccharomyces cerevisiae to the different inhibitors generated during the pretreatment of lignocellulosic material is driving the development of new strains with higher inhibitor tolerances. The objective of this study is to assess, using flow cytometry, how three common inhibitors (vanillin, furfural, and acetic acid) affect the membrane potential, the membrane permeability and the concentration of reactive oxygen species (ROS) during the different fermentations. The membrane potential decreased during the detoxification phase and reflected on the different mechanisms of the toxicity of the inhibitors. While vanillin and furfural caused a metabolic inhibition and a gradual depolarization, acetic acid toxicity was related to fast acidification of the cytosol, causing an immediate depolarization. In the absence of acetic acid, ethanol increased membrane permeability, indicating a possible acquired tolerance to ethanol due to an adaptive response to acetic acid. The intracellular ROS concentration also increased in the presence of the inhibitors, indicating oxidative stress. Measuring these features with flow cytometry allows a real-time assessment of the stress of a cell culture, which can be used in the development of new yeast strains and to design new propagation strategies to pre-adapt the cell cultures to the inhibitors.
format article
author Pau Cabaneros López
Chuantao Peng
Nils Arneborg
Helena Junicke
Krist V. Gernaey
author_facet Pau Cabaneros López
Chuantao Peng
Nils Arneborg
Helena Junicke
Krist V. Gernaey
author_sort Pau Cabaneros López
title Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
title_short Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
title_full Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
title_fullStr Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
title_full_unstemmed Analysis of the response of the cell membrane of Saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
title_sort analysis of the response of the cell membrane of saccharomyces cerevisiae during the detoxification of common lignocellulosic inhibitors
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/c58e22cfc8cc4f4e973391a7e709ac3e
work_keys_str_mv AT paucabaneroslopez analysisoftheresponseofthecellmembraneofsaccharomycescerevisiaeduringthedetoxificationofcommonlignocellulosicinhibitors
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AT nilsarneborg analysisoftheresponseofthecellmembraneofsaccharomycescerevisiaeduringthedetoxificationofcommonlignocellulosicinhibitors
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