Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato

Abstract Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J. K. Morton) was investigated. Five genotypes of Chinese potato were raised in Murashige and Skoog (MS) basal medium co...

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Autores principales:	Manas Ranjan Sahoo, Tongbram Roshni Devi, Madhumita Dasgupta, Potshangbam Nongdam, Narendra Prakash
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c58f414f38214c83b50b1676674e35e6
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spelling	oai:doaj.org-article:c58f414f38214c83b50b1676674e35e62021-12-02T17:04:08ZReactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato10.1038/s41598-020-62317-z2045-2322https://doaj.org/article/c58f414f38214c83b50b1676674e35e62020-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-62317-zhttps://doaj.org/toc/2045-2322Abstract Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J. K. Morton) was investigated. Five genotypes of Chinese potato were raised in Murashige and Skoog (MS) basal medium containing 6–benzylaminopurine (BAP, 1 mg L–1) along with various concentrations of PEG–6000 mediated stress conditions (0, –0.2 and –0.5 MPa) and evaluated for osmotic stress tolerance in vitro. The medium containing PEG–6000 had a detrimental effect on plantlet growth and development while compared with the control. Accumulation of H2O2 was lower in Sreedhara and Subala and higher in Nidhi under PEG stress, which was evident by in situ detection in leaves. Lipid peroxidation product such as malondialdehyde (MDA) content was increased due to PEG stress which was more in susceptible genotype than that in tolerant ones. An enhanced ROS–scavenging antioxidant enzyme was observed under stress with respect to the control. The enzymes of ascorbate–glutathione cycle showed an important role in scavenging ROS. The imposition of PEG stress also increased the non–enzymatic antioxidants viz., the ascorbate and reduced glutathione content which was prominent in tolerant genotypes in comparison to susceptible. The present study indicated that, Sreedhara and Subala showed more tolerance to osmotic stress with better ROS scavenging machineries which would be the lines of interest for augmenting future breeding strategies in this climate resilient minor tuber crop.Manas Ranjan SahooTongbram Roshni DeviMadhumita DasguptaPotshangbam NongdamNarendra PrakashNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manas Ranjan Sahoo Tongbram Roshni Devi Madhumita Dasgupta Potshangbam Nongdam Narendra Prakash Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
description	Abstract Influence of polyethylene glycol (PEG) mediated osmotic stress on reactive oxygen species (ROS) scavenging machinery of Chinese potato (Solenostemon rotundifolius (Poir.) J. K. Morton) was investigated. Five genotypes of Chinese potato were raised in Murashige and Skoog (MS) basal medium containing 6–benzylaminopurine (BAP, 1 mg L–1) along with various concentrations of PEG–6000 mediated stress conditions (0, –0.2 and –0.5 MPa) and evaluated for osmotic stress tolerance in vitro. The medium containing PEG–6000 had a detrimental effect on plantlet growth and development while compared with the control. Accumulation of H2O2 was lower in Sreedhara and Subala and higher in Nidhi under PEG stress, which was evident by in situ detection in leaves. Lipid peroxidation product such as malondialdehyde (MDA) content was increased due to PEG stress which was more in susceptible genotype than that in tolerant ones. An enhanced ROS–scavenging antioxidant enzyme was observed under stress with respect to the control. The enzymes of ascorbate–glutathione cycle showed an important role in scavenging ROS. The imposition of PEG stress also increased the non–enzymatic antioxidants viz., the ascorbate and reduced glutathione content which was prominent in tolerant genotypes in comparison to susceptible. The present study indicated that, Sreedhara and Subala showed more tolerance to osmotic stress with better ROS scavenging machineries which would be the lines of interest for augmenting future breeding strategies in this climate resilient minor tuber crop.
format	article
author	Manas Ranjan Sahoo Tongbram Roshni Devi Madhumita Dasgupta Potshangbam Nongdam Narendra Prakash
author_facet	Manas Ranjan Sahoo Tongbram Roshni Devi Madhumita Dasgupta Potshangbam Nongdam Narendra Prakash
author_sort	Manas Ranjan Sahoo
title	Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
title_short	Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
title_full	Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
title_fullStr	Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
title_full_unstemmed	Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato
title_sort	reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in chinese potato
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c58f414f38214c83b50b1676674e35e6
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Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato

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