De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantific...

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Autores principales: Limin Shi, Chenxu Yan, Zhiqian Guo, Weijie Chi, Jingle Wei, Weimin Liu, Xiaogang Liu, He Tian, Wei-Hong Zhu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/c5968773923340a4b68bb4e77ddcc06d
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spelling oai:doaj.org-article:c5968773923340a4b68bb4e77ddcc06d2021-12-02T14:40:52ZDe novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules10.1038/s41467-020-14615-32041-1723https://doaj.org/article/c5968773923340a4b68bb4e77ddcc06d2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14615-3https://doaj.org/toc/2041-1723Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantification.Limin ShiChenxu YanZhiqian GuoWeijie ChiJingle WeiWeimin LiuXiaogang LiuHe TianWei-Hong ZhuNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Limin Shi
Chenxu Yan
Zhiqian Guo
Weijie Chi
Jingle Wei
Weimin Liu
Xiaogang Liu
He Tian
Wei-Hong Zhu
De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
description Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantification.
format article
author Limin Shi
Chenxu Yan
Zhiqian Guo
Weijie Chi
Jingle Wei
Weimin Liu
Xiaogang Liu
He Tian
Wei-Hong Zhu
author_facet Limin Shi
Chenxu Yan
Zhiqian Guo
Weijie Chi
Jingle Wei
Weimin Liu
Xiaogang Liu
He Tian
Wei-Hong Zhu
author_sort Limin Shi
title De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
title_short De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
title_full De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
title_fullStr De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
title_full_unstemmed De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules
title_sort de novo strategy with engineering anti-kasha/kasha fluorophores enables reliable ratiometric quantification of biomolecules
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/c5968773923340a4b68bb4e77ddcc06d
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