Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea

Methane seeps are chemosynthetic ecosystems in the deep-sea environment. Microbial community structures have been extensively studied in the seepage-affected sediments and investigation in the water column above the seeping sites is still lacking. In this study, prokaryotic communities in the bottom...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaopeng Li, Zehan Dai, Pengfei Di, Junxi Feng, Jun Tao, Duofu Chen, Niu Li, Yazi Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	methane seeps various seepage intensities oil seepage microbial communities bottom water South China Sea Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/c5969d82295e431c9c0f46c67f2cc874
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c5969d82295e431c9c0f46c67f2cc874
record_format	dspace
spelling	oai:doaj.org-article:c5969d82295e431c9c0f46c67f2cc8742021-11-15T05:04:20ZDistinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea2296-774510.3389/fmars.2021.753952https://doaj.org/article/c5969d82295e431c9c0f46c67f2cc8742021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.753952/fullhttps://doaj.org/toc/2296-7745Methane seeps are chemosynthetic ecosystems in the deep-sea environment. Microbial community structures have been extensively studied in the seepage-affected sediments and investigation in the water column above the seeping sites is still lacking. In this study, prokaryotic communities in the bottom water about 50 cm from the seabed at methane seeps with various seepage intensities in Haima, South China Sea were comparatively studied by using 16S ribosomal RNA gene sequencing. These sites were assigned based on their distinct methane content levels and seafloor landscapes as the non-seepage (NS) site, low-intensity seepage (LIS) site, and high-intensity seepage (HIS) site. The abundances of the dominant phyla Proteobacteria, Bacteroidetes, and Actinobacteria differed significantly between NS and the two seepage sites (p < 0.05). Alpha diversity differed among the three sites with the HIS site showing the lowest community diversity. Principal component analysis revealed highly divergent bacterial community structures at three sites. Many environmental variables including temperature, alkalinity, pH, methane, dissolved organic carbon (DOC), and inorganic nutrients were measured. Redundancy analysis indicated that methane content is the key environmental factor driving bacterial community variation (p = 0.001). Linear discriminant analysis effect size analysis identified various differentially enriched genera at the LIS and HIS sites. Phylogenetic analysis revealed close phylogenetic relationship among the operational taxonomic units of these genera with known oil-degrading species, indicating oil seepage may occur at the Haima cold seeps. Co-occurrence networks indicated that the strength of microbial interactions was weakest at the HIS site. This study represents a comprehensive comparison of microbial profiles in the water column of cold seeps in the SCS, revealing that the seepage intensity has a strong impact on bacterial community dynamics.Xiaopeng LiZehan DaiPengfei DiJunxi FengJun TaoDuofu ChenNiu LiYazi LiFrontiers Media S.A.articlemethane seepsvarious seepage intensitiesoil seepagemicrobial communitiesbottom waterSouth China SeaScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	methane seeps various seepage intensities oil seepage microbial communities bottom water South China Sea Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	methane seeps various seepage intensities oil seepage microbial communities bottom water South China Sea Science Q General. Including nature conservation, geographical distribution QH1-199.5 Xiaopeng Li Zehan Dai Pengfei Di Junxi Feng Jun Tao Duofu Chen Niu Li Yazi Li Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
description	Methane seeps are chemosynthetic ecosystems in the deep-sea environment. Microbial community structures have been extensively studied in the seepage-affected sediments and investigation in the water column above the seeping sites is still lacking. In this study, prokaryotic communities in the bottom water about 50 cm from the seabed at methane seeps with various seepage intensities in Haima, South China Sea were comparatively studied by using 16S ribosomal RNA gene sequencing. These sites were assigned based on their distinct methane content levels and seafloor landscapes as the non-seepage (NS) site, low-intensity seepage (LIS) site, and high-intensity seepage (HIS) site. The abundances of the dominant phyla Proteobacteria, Bacteroidetes, and Actinobacteria differed significantly between NS and the two seepage sites (p < 0.05). Alpha diversity differed among the three sites with the HIS site showing the lowest community diversity. Principal component analysis revealed highly divergent bacterial community structures at three sites. Many environmental variables including temperature, alkalinity, pH, methane, dissolved organic carbon (DOC), and inorganic nutrients were measured. Redundancy analysis indicated that methane content is the key environmental factor driving bacterial community variation (p = 0.001). Linear discriminant analysis effect size analysis identified various differentially enriched genera at the LIS and HIS sites. Phylogenetic analysis revealed close phylogenetic relationship among the operational taxonomic units of these genera with known oil-degrading species, indicating oil seepage may occur at the Haima cold seeps. Co-occurrence networks indicated that the strength of microbial interactions was weakest at the HIS site. This study represents a comprehensive comparison of microbial profiles in the water column of cold seeps in the SCS, revealing that the seepage intensity has a strong impact on bacterial community dynamics.
format	article
author	Xiaopeng Li Zehan Dai Pengfei Di Junxi Feng Jun Tao Duofu Chen Niu Li Yazi Li
author_facet	Xiaopeng Li Zehan Dai Pengfei Di Junxi Feng Jun Tao Duofu Chen Niu Li Yazi Li
author_sort	Xiaopeng Li
title	Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
title_short	Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
title_full	Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
title_fullStr	Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
title_full_unstemmed	Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea
title_sort	distinct bottom-water bacterial communities at methane seeps with various seepage intensities in haima, south china sea
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c5969d82295e431c9c0f46c67f2cc874
work_keys_str_mv	AT xiaopengli distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT zehandai distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT pengfeidi distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT junxifeng distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT juntao distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT duofuchen distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT niuli distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea AT yazili distinctbottomwaterbacterialcommunitiesatmethaneseepswithvariousseepageintensitiesinhaimasouthchinasea
_version_	1718428829833232384

Distinct Bottom-Water Bacterial Communities at Methane Seeps With Various Seepage Intensities in Haima, South China Sea

Ejemplares similares