Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging

Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes...

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Autores principales:	Yingying Jing, Chenshuang Zhang, Bin Yu, Danying Lin, Junle Qu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	super-resolution techniques in vivo imaging labeling strategies near-infrared fluorescent probes in vivo applications Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c59a785844424175a22e41cb9f4883e4
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id	oai:doaj.org-article:c59a785844424175a22e41cb9f4883e4
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spelling	oai:doaj.org-article:c59a785844424175a22e41cb9f4883e42021-11-22T08:26:38ZSuper-Resolution Microscopy: Shedding New Light on In Vivo Imaging2296-264610.3389/fchem.2021.746900https://doaj.org/article/c59a785844424175a22e41cb9f4883e42021-09-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.746900/fullhttps://doaj.org/toc/2296-2646Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging.Yingying JingChenshuang ZhangBin YuDanying LinJunle QuFrontiers Media S.A.articlesuper-resolution techniquesin vivo imaginglabeling strategiesnear-infrared fluorescent probesin vivo applicationsChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	super-resolution techniques in vivo imaging labeling strategies near-infrared fluorescent probes in vivo applications Chemistry QD1-999
spellingShingle	super-resolution techniques in vivo imaging labeling strategies near-infrared fluorescent probes in vivo applications Chemistry QD1-999 Yingying Jing Chenshuang Zhang Bin Yu Danying Lin Junle Qu Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
description	Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging.
format	article
author	Yingying Jing Chenshuang Zhang Bin Yu Danying Lin Junle Qu
author_facet	Yingying Jing Chenshuang Zhang Bin Yu Danying Lin Junle Qu
author_sort	Yingying Jing
title	Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_short	Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_full	Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_fullStr	Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_full_unstemmed	Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging
title_sort	super-resolution microscopy: shedding new light on in vivo imaging
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c59a785844424175a22e41cb9f4883e4
work_keys_str_mv	AT yingyingjing superresolutionmicroscopysheddingnewlightoninvivoimaging AT chenshuangzhang superresolutionmicroscopysheddingnewlightoninvivoimaging AT binyu superresolutionmicroscopysheddingnewlightoninvivoimaging AT danyinglin superresolutionmicroscopysheddingnewlightoninvivoimaging AT junlequ superresolutionmicroscopysheddingnewlightoninvivoimaging
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Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging

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