Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines

Merlis P Alvarez-Berríos,1 Amalchi Castillo,1 Carlos Rinaldi,1–3 Madeline Torres-Lugo1 1Department of Chemical Engineering, University of Puerto Rico, Mayagüez, Puerto Rico; 2J Crayton Pruitt Family Department of Biomedical Engineering, 3Department of Chemical Engineer...

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Autores principales:	Alvarez-Berríos MP, Castillo A, Rinaldi C, Torres-Lugo M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c59c5ac2dd79442e92fd733b8e86e125
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spelling	oai:doaj.org-article:c59c5ac2dd79442e92fd733b8e86e1252021-12-02T07:25:54ZMagnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines1178-2013https://doaj.org/article/c59c5ac2dd79442e92fd733b8e86e1252013-12-01T00:00:00Zhttp://www.dovepress.com/magnetic-fluid-hyperthermia-enhances-cytotoxicity-of-bortezomib-in-sen-a15359https://doaj.org/toc/1178-2013 Merlis P Alvarez-Berríos,1 Amalchi Castillo,1 Carlos Rinaldi,1–3 Madeline Torres-Lugo1 1Department of Chemical Engineering, University of Puerto Rico, Mayagüez, Puerto Rico; 2J Crayton Pruitt Family Department of Biomedical Engineering, 3Department of Chemical Engineering, University of Florida, Gainesville, FL, USA Abstract: The proteasome inhibitor bortezomib (BZ) has shown promising results in some types of cancer, but in others it has had minimal activity. Recent studies have reported enhanced efficacy of BZ when combined with hyperthermia. However, the use of magnetic nanoparticles to induce hyperthermia in combination with BZ has not been reported. This novel hyperthermia modality has shown better potentiation of chemotherapeutics over other types of hyperthermia. We hypothesized that inducing hyperthermia via magnetic nanoparticles (MFH) would enhance the cytotoxicity of BZ in BZ-sensitive and BZ-resistant cancer cells more effectively than hyperthermia using a hot water bath (HWH). Studies were conducted using BZ in combination with MFH in two BZ-sensitive cell lines (MDA-MB-468, Caco-2), and one BZ-resistant cell line (A2780) at two different conditions, ie, 43°C for 30 minutes and 45°C for 30 minutes. These experiments were compared with combined application of HWH and BZ. The results indicate enhanced potentiation between hyperthermic treatment and BZ. MFH combined with BZ induced cytotoxicity in sensitive and resistant cell lines to a greater extent than HWH under the same treatment conditions. The observation that MFH sensitizes BZ-resistant cell lines makes this approach a potentially effective anticancer therapy platform. Keywords: magnetic fluid hyperthermia, hot water hyperthermia, BZ, enhanced cytotoxicity, thermal sensitizationAlvarez-Berríos MPCastillo ARinaldi CTorres-Lugo MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 145-153 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alvarez-Berríos MP Castillo A Rinaldi C Torres-Lugo M Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
description	Merlis P Alvarez-Berríos,1 Amalchi Castillo,1 Carlos Rinaldi,1–3 Madeline Torres-Lugo1 1Department of Chemical Engineering, University of Puerto Rico, Mayagüez, Puerto Rico; 2J Crayton Pruitt Family Department of Biomedical Engineering, 3Department of Chemical Engineering, University of Florida, Gainesville, FL, USA Abstract: The proteasome inhibitor bortezomib (BZ) has shown promising results in some types of cancer, but in others it has had minimal activity. Recent studies have reported enhanced efficacy of BZ when combined with hyperthermia. However, the use of magnetic nanoparticles to induce hyperthermia in combination with BZ has not been reported. This novel hyperthermia modality has shown better potentiation of chemotherapeutics over other types of hyperthermia. We hypothesized that inducing hyperthermia via magnetic nanoparticles (MFH) would enhance the cytotoxicity of BZ in BZ-sensitive and BZ-resistant cancer cells more effectively than hyperthermia using a hot water bath (HWH). Studies were conducted using BZ in combination with MFH in two BZ-sensitive cell lines (MDA-MB-468, Caco-2), and one BZ-resistant cell line (A2780) at two different conditions, ie, 43°C for 30 minutes and 45°C for 30 minutes. These experiments were compared with combined application of HWH and BZ. The results indicate enhanced potentiation between hyperthermic treatment and BZ. MFH combined with BZ induced cytotoxicity in sensitive and resistant cell lines to a greater extent than HWH under the same treatment conditions. The observation that MFH sensitizes BZ-resistant cell lines makes this approach a potentially effective anticancer therapy platform. Keywords: magnetic fluid hyperthermia, hot water hyperthermia, BZ, enhanced cytotoxicity, thermal sensitization
format	article
author	Alvarez-Berríos MP Castillo A Rinaldi C Torres-Lugo M
author_facet	Alvarez-Berríos MP Castillo A Rinaldi C Torres-Lugo M
author_sort	Alvarez-Berríos MP
title	Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
title_short	Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
title_full	Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
title_fullStr	Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
title_full_unstemmed	Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
title_sort	magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/c59c5ac2dd79442e92fd733b8e86e125
work_keys_str_mv	AT alvarezberriacuteosmp magneticfluidhyperthermiaenhancescytotoxicityofbortezomibinsensitiveandresistantcancercelllines AT castilloa magneticfluidhyperthermiaenhancescytotoxicityofbortezomibinsensitiveandresistantcancercelllines AT rinaldic magneticfluidhyperthermiaenhancescytotoxicityofbortezomibinsensitiveandresistantcancercelllines AT torreslugom magneticfluidhyperthermiaenhancescytotoxicityofbortezomibinsensitiveandresistantcancercelllines
_version_	1718399424959348736

Magnetic fluid hyperthermia enhances cytotoxicity of bortezomib in sensitive and resistant cancer cell lines

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