Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)

Transformation two cry genes (cryIB-cryIAa) and transformation with a single the cry1B gene under the control of a wound-inducible maize proteinase inhibitor gene (mpi) promoter were two approach...

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Autores principales:	N. Usyati, Damayanti Buchori, Syafrida Manuwoto, Purnama Hidayat, Inez H. Slamet -Loedin
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2016
Materias:	Effectiveness, resistance value, transgenic rice, S. incertulas. Zoology QL1-991
Acceso en línea:	https://doaj.org/article/c5a83f5a9aab4931aa6d7ff18f444d28
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spelling	oai:doaj.org-article:c5a83f5a9aab4931aa6d7ff18f444d282021-12-02T08:37:31ZKeefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)1829-77222089-025710.5994/jei.6.1.30https://doaj.org/article/c5a83f5a9aab4931aa6d7ff18f444d282016-12-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/253https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257Transformation two <em>cry</em> genes (<em>cry</em>IB-<em>cry</em>IAa) and transformation with a single the <em>cry</em>1B gene under the control of a wound-inducible maize proteinase inhibitor gene (<em>mpi</em>) promoter were two approaches that were used to get resistant rice to the rice stemborer which may be had a durable resistance. To obtain information on the effectiveness of seven transgenic rice lines to the rice yellow stemborer <em>S. incertulas</em>, a test was conducted in greenhouse. The seven lines were 1). line 4.2.3 and 2) line 4.2.4 both contain fusion of two <em>cry</em> genes (<em>cry</em>IB-<em>cry</em>IAa); 3) line 3R9 and 4) line 3R7 lines both contain of <em>mpi</em>-<em>cry</em>IB gene; and 5) line 6.11 contains of <em>cry</em>IAb gene by particle bombardment, 6) line DT-<em>cry</em> (Azygous) that do not contain <em>cry</em> gene (null), and 7) DT-<em>cry</em> line contains <em>cry</em>IAb gene by <em>Agrobacterium</em>, and as a negative control, we used three non transgenic rice varieties i.e., Rojolele, Cilosari, and Ciherang. The result showed that transgenic rice lines, except DT-<em>cry</em> and DT-<em>cry</em> (Azygous) lines were effective to suppress damage by the insect, and showed an inhibition effect on the growth of <em>S. incertulas</em>, and had a high level ofresistance than non transgenic rice varieties had. There were differences on resistance value/level among transgenic rice lines. Based on the resistance value, 6.11 line was the highest followed by 4.2.4 line and 3R7 line, these lines were categorized as high resistance. Transgenic rice-4.2.3 line and 3R9 line were categorized as moderat resistance. DT-<em>cry</em> and DT-<em>cry</em> (Azygous) lines were susceptible.N. UsyatiDamayanti BuchoriSyafrida ManuwotoPurnama HidayatInez H. Slamet -LoedinThe Entomological Society of IndonesiaarticleEffectiveness, resistance value, transgenic rice, S. incertulas.ZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 6, Iss 1 (2016)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Effectiveness, resistance value, transgenic rice, S. incertulas. Zoology QL1-991
spellingShingle	Effectiveness, resistance value, transgenic rice, S. incertulas. Zoology QL1-991 N. Usyati Damayanti Buchori Syafrida Manuwoto Purnama Hidayat Inez H. Slamet -Loedin Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
description	Transformation two <em>cry</em> genes (<em>cry</em>IB-<em>cry</em>IAa) and transformation with a single the <em>cry</em>1B gene under the control of a wound-inducible maize proteinase inhibitor gene (<em>mpi</em>) promoter were two approaches that were used to get resistant rice to the rice stemborer which may be had a durable resistance. To obtain information on the effectiveness of seven transgenic rice lines to the rice yellow stemborer <em>S. incertulas</em>, a test was conducted in greenhouse. The seven lines were 1). line 4.2.3 and 2) line 4.2.4 both contain fusion of two <em>cry</em> genes (<em>cry</em>IB-<em>cry</em>IAa); 3) line 3R9 and 4) line 3R7 lines both contain of <em>mpi</em>-<em>cry</em>IB gene; and 5) line 6.11 contains of <em>cry</em>IAb gene by particle bombardment, 6) line DT-<em>cry</em> (Azygous) that do not contain <em>cry</em> gene (null), and 7) DT-<em>cry</em> line contains <em>cry</em>IAb gene by <em>Agrobacterium</em>, and as a negative control, we used three non transgenic rice varieties i.e., Rojolele, Cilosari, and Ciherang. The result showed that transgenic rice lines, except DT-<em>cry</em> and DT-<em>cry</em> (Azygous) lines were effective to suppress damage by the insect, and showed an inhibition effect on the growth of <em>S. incertulas</em>, and had a high level ofresistance than non transgenic rice varieties had. There were differences on resistance value/level among transgenic rice lines. Based on the resistance value, 6.11 line was the highest followed by 4.2.4 line and 3R7 line, these lines were categorized as high resistance. Transgenic rice-4.2.3 line and 3R9 line were categorized as moderat resistance. DT-<em>cry</em> and DT-<em>cry</em> (Azygous) lines were susceptible.
format	article
author	N. Usyati Damayanti Buchori Syafrida Manuwoto Purnama Hidayat Inez H. Slamet -Loedin
author_facet	N. Usyati Damayanti Buchori Syafrida Manuwoto Purnama Hidayat Inez H. Slamet -Loedin
author_sort	N. Usyati
title	Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
title_short	Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
title_full	Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
title_fullStr	Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
title_full_unstemmed	Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)
title_sort	keefektivan padi transgenik terhadap hama penggerek batang padi kuning scirpophaga incertulas (walker) (lepidoptera: crambidae)
publisher	The Entomological Society of Indonesia
publishDate	2016
url	https://doaj.org/article/c5a83f5a9aab4931aa6d7ff18f444d28
work_keys_str_mv	AT nusyati keefektivanpaditransgenikterhadaphamapenggerekbatangpadikuningscirpophagaincertulaswalkerlepidopteracrambidae AT damayantibuchori keefektivanpaditransgenikterhadaphamapenggerekbatangpadikuningscirpophagaincertulaswalkerlepidopteracrambidae AT syafridamanuwoto keefektivanpaditransgenikterhadaphamapenggerekbatangpadikuningscirpophagaincertulaswalkerlepidopteracrambidae AT purnamahidayat keefektivanpaditransgenikterhadaphamapenggerekbatangpadikuningscirpophagaincertulaswalkerlepidopteracrambidae AT inezhslametloedin keefektivanpaditransgenikterhadaphamapenggerekbatangpadikuningscirpophagaincertulaswalkerlepidopteracrambidae
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Keefektivan padi transgenik terhadap hama penggerek batang padi kuning Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae)

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