Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.
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Nature Portfolio
2020
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oai:doaj.org-article:c5b1842b3a754bafbbaa405ec63517202021-12-02T16:49:29ZSpectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates10.1038/s41467-020-15618-w2041-1723https://doaj.org/article/c5b1842b3a754bafbbaa405ec63517202020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15618-whttps://doaj.org/toc/2041-1723Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.Sebastian KarpfCarson T. RicheDino Di CarloAnubhuti GoelWilliam A. ZeigerAnand SureshCarlos Portera-CailliauBahram JalaliNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020) |
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Science Q |
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Science Q Sebastian Karpf Carson T. Riche Dino Di Carlo Anubhuti Goel William A. Zeiger Anand Suresh Carlos Portera-Cailliau Bahram Jalali Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
description |
Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates. |
format |
article |
author |
Sebastian Karpf Carson T. Riche Dino Di Carlo Anubhuti Goel William A. Zeiger Anand Suresh Carlos Portera-Cailliau Bahram Jalali |
author_facet |
Sebastian Karpf Carson T. Riche Dino Di Carlo Anubhuti Goel William A. Zeiger Anand Suresh Carlos Portera-Cailliau Bahram Jalali |
author_sort |
Sebastian Karpf |
title |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
title_short |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
title_full |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
title_fullStr |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
title_full_unstemmed |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
title_sort |
spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/c5b1842b3a754bafbbaa405ec6351720 |
work_keys_str_mv |
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1718383302291750912 |