Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.

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Autores principales: Sebastian Karpf, Carson T. Riche, Dino Di Carlo, Anubhuti Goel, William A. Zeiger, Anand Suresh, Carlos Portera-Cailliau, Bahram Jalali
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Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/c5b1842b3a754bafbbaa405ec6351720
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spelling oai:doaj.org-article:c5b1842b3a754bafbbaa405ec63517202021-12-02T16:49:29ZSpectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates10.1038/s41467-020-15618-w2041-1723https://doaj.org/article/c5b1842b3a754bafbbaa405ec63517202020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15618-whttps://doaj.org/toc/2041-1723Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.Sebastian KarpfCarson T. RicheDino Di CarloAnubhuti GoelWilliam A. ZeigerAnand SureshCarlos Portera-CailliauBahram JalaliNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sebastian Karpf
Carson T. Riche
Dino Di Carlo
Anubhuti Goel
William A. Zeiger
Anand Suresh
Carlos Portera-Cailliau
Bahram Jalali
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
description Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.
format article
author Sebastian Karpf
Carson T. Riche
Dino Di Carlo
Anubhuti Goel
William A. Zeiger
Anand Suresh
Carlos Portera-Cailliau
Bahram Jalali
author_facet Sebastian Karpf
Carson T. Riche
Dino Di Carlo
Anubhuti Goel
William A. Zeiger
Anand Suresh
Carlos Portera-Cailliau
Bahram Jalali
author_sort Sebastian Karpf
title Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
title_short Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
title_full Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
title_fullStr Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
title_full_unstemmed Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
title_sort spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/c5b1842b3a754bafbbaa405ec6351720
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