Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.

Insect seminal fluid proteins are powerful modulators of many aspects of female physiology and behaviour including longevity, egg production, sperm storage, and remating. The crucial role of these proteins in reproduction makes them promising targets for developing tools aimed at reducing the popula...

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Autores principales:	David W Rogers, Francesco Baldini, Francesca Battaglia, Maria Panico, Anne Dell, Howard R Morris, Flaminia Catteruccia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c5b2451f5aff4caa8f44d50830c6a680
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spelling	oai:doaj.org-article:c5b2451f5aff4caa8f44d50830c6a6802021-11-25T05:34:27ZTransglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.1544-91731545-788510.1371/journal.pbio.1000272https://doaj.org/article/c5b2451f5aff4caa8f44d50830c6a6802009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20027206/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Insect seminal fluid proteins are powerful modulators of many aspects of female physiology and behaviour including longevity, egg production, sperm storage, and remating. The crucial role of these proteins in reproduction makes them promising targets for developing tools aimed at reducing the population sizes of vectors of disease. In the malaria mosquito Anopheles gambiae, seminal secretions produced by the male accessory glands (MAGs) are transferred to females in the form of a coagulated mass called the mating plug. The potential of seminal fluid proteins as tools for mosquito control demands that we improve our limited understanding of the composition and function of the plug. Here, we show that the plug is a key determinant of An. gambiae reproductive success. We uncover the composition of the plug and demonstrate it is formed through the cross-linking of seminal proteins mediated by a MAG-specific transglutaminase (TGase), a mechanism remarkably similar to mammalian semen coagulation. Interfering with TGase expression in males inhibits plug formation and transfer, and prevents females from storing sperm with obvious consequences for fertility. Moreover, we show that the MAG-specific TGase is restricted to the anopheline lineage, where it functions to promote sperm storage rather than as a mechanical barrier to re-insemination. Taken together, these data represent a major advance in our understanding of the factors shaping Anopheles reproductive biology.David W RogersFrancesco BaldiniFrancesca BattagliaMaria PanicoAnne DellHoward R MorrisFlaminia CatterucciaPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 12, p e1000272 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 David W Rogers Francesco Baldini Francesca Battaglia Maria Panico Anne Dell Howard R Morris Flaminia Catteruccia Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
description	Insect seminal fluid proteins are powerful modulators of many aspects of female physiology and behaviour including longevity, egg production, sperm storage, and remating. The crucial role of these proteins in reproduction makes them promising targets for developing tools aimed at reducing the population sizes of vectors of disease. In the malaria mosquito Anopheles gambiae, seminal secretions produced by the male accessory glands (MAGs) are transferred to females in the form of a coagulated mass called the mating plug. The potential of seminal fluid proteins as tools for mosquito control demands that we improve our limited understanding of the composition and function of the plug. Here, we show that the plug is a key determinant of An. gambiae reproductive success. We uncover the composition of the plug and demonstrate it is formed through the cross-linking of seminal proteins mediated by a MAG-specific transglutaminase (TGase), a mechanism remarkably similar to mammalian semen coagulation. Interfering with TGase expression in males inhibits plug formation and transfer, and prevents females from storing sperm with obvious consequences for fertility. Moreover, we show that the MAG-specific TGase is restricted to the anopheline lineage, where it functions to promote sperm storage rather than as a mechanical barrier to re-insemination. Taken together, these data represent a major advance in our understanding of the factors shaping Anopheles reproductive biology.
format	article
author	David W Rogers Francesco Baldini Francesca Battaglia Maria Panico Anne Dell Howard R Morris Flaminia Catteruccia
author_facet	David W Rogers Francesco Baldini Francesca Battaglia Maria Panico Anne Dell Howard R Morris Flaminia Catteruccia
author_sort	David W Rogers
title	Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
title_short	Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
title_full	Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
title_fullStr	Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
title_full_unstemmed	Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
title_sort	transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c5b2451f5aff4caa8f44d50830c6a680
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Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.

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