Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis
Abstract The small intestinal mucosa-associated microbiota (MAM) can potentially impact the etiology of primary biliary cholangitis (PBC). Herein, we investigate the MAM profile to determine its association with liver pathology in patients with PBC. Thirty-four patients with PBC and 21 healthy contr...
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2021
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oai:doaj.org-article:c5b37c00c263485e80e70193a56a9e972021-12-02T18:37:08ZIleal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis10.1038/s41598-021-99314-92045-2322https://doaj.org/article/c5b37c00c263485e80e70193a56a9e972021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99314-9https://doaj.org/toc/2045-2322Abstract The small intestinal mucosa-associated microbiota (MAM) can potentially impact the etiology of primary biliary cholangitis (PBC). Herein, we investigate the MAM profile to determine its association with liver pathology in patients with PBC. Thirty-four patients with PBC and 21 healthy controls who underwent colonoscopy at our hospital were enrolled in our study. We performed 16S ribosomal RNA gene sequencing of MAM samples obtained from the mucosa of the terminal ileum and examined the relationship between the abundance of ileal MAM and chronic nonsuppurative destructive cholangitis using liver specimens from patients with PBC. There was a significant reduction in microbial diversity within individuals with PBC (P = 0.039). Dysbiosis of ileal MAM was observed in patients with PBC, with a characteristic overgrowth of Sphingomonadaceae and Pseudomonas. Multivariate analysis showed that the overgrowth of Sphingomonadaceae and Pseudomonas is an independent association factor for PBC (P = 0.0429, P = 0.026). Moreover, the abundance of Sphingomonadaceae was associated with chronic nonsuppurative destructive cholangitis in PBC (P = 0.00981). The overgrowth of Sphingomonadaceae and Pseudomonas in ileal MAM was found in patients with PBC. Sphingomonadaceae may be associated with the pathological development of PBC.Shogo KitahataYasunori YamamotoOsamu YoshidaYoshio TokumotoTomoe KawamuraShinya FurukawaTeru KumagiMasashi HirookaEiji TakeshitaMasanori AbeYoshiou IkedaYoichi HiasaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Shogo Kitahata Yasunori Yamamoto Osamu Yoshida Yoshio Tokumoto Tomoe Kawamura Shinya Furukawa Teru Kumagi Masashi Hirooka Eiji Takeshita Masanori Abe Yoshiou Ikeda Yoichi Hiasa Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| description |
Abstract The small intestinal mucosa-associated microbiota (MAM) can potentially impact the etiology of primary biliary cholangitis (PBC). Herein, we investigate the MAM profile to determine its association with liver pathology in patients with PBC. Thirty-four patients with PBC and 21 healthy controls who underwent colonoscopy at our hospital were enrolled in our study. We performed 16S ribosomal RNA gene sequencing of MAM samples obtained from the mucosa of the terminal ileum and examined the relationship between the abundance of ileal MAM and chronic nonsuppurative destructive cholangitis using liver specimens from patients with PBC. There was a significant reduction in microbial diversity within individuals with PBC (P = 0.039). Dysbiosis of ileal MAM was observed in patients with PBC, with a characteristic overgrowth of Sphingomonadaceae and Pseudomonas. Multivariate analysis showed that the overgrowth of Sphingomonadaceae and Pseudomonas is an independent association factor for PBC (P = 0.0429, P = 0.026). Moreover, the abundance of Sphingomonadaceae was associated with chronic nonsuppurative destructive cholangitis in PBC (P = 0.00981). The overgrowth of Sphingomonadaceae and Pseudomonas in ileal MAM was found in patients with PBC. Sphingomonadaceae may be associated with the pathological development of PBC. |
| format |
article |
| author |
Shogo Kitahata Yasunori Yamamoto Osamu Yoshida Yoshio Tokumoto Tomoe Kawamura Shinya Furukawa Teru Kumagi Masashi Hirooka Eiji Takeshita Masanori Abe Yoshiou Ikeda Yoichi Hiasa |
| author_facet |
Shogo Kitahata Yasunori Yamamoto Osamu Yoshida Yoshio Tokumoto Tomoe Kawamura Shinya Furukawa Teru Kumagi Masashi Hirooka Eiji Takeshita Masanori Abe Yoshiou Ikeda Yoichi Hiasa |
| author_sort |
Shogo Kitahata |
| title |
Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| title_short |
Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| title_full |
Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| title_fullStr |
Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| title_full_unstemmed |
Ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| title_sort |
ileal mucosa-associated microbiota overgrowth associated with pathogenesis of primary biliary cholangitis |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c5b37c00c263485e80e70193a56a9e97 |
| work_keys_str_mv |
AT shogokitahata ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT yasunoriyamamoto ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT osamuyoshida ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT yoshiotokumoto ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT tomoekawamura ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT shinyafurukawa ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT terukumagi ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT masashihirooka ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT eijitakeshita ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT masanoriabe ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT yoshiouikeda ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis AT yoichihiasa ilealmucosaassociatedmicrobiotaovergrowthassociatedwithpathogenesisofprimarybiliarycholangitis |
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1718377824848445440 |