Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array

Abstract Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging d...

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Autores principales:	Ching-Te Kuo, Jong-Yueh Wang, Yu-Fen Lin, Andrew M. Wo, Benjamin P. C. Chen, Hsinyu Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5c1140c31ad4e1ba412c91936e1e05d
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spelling	oai:doaj.org-article:c5c1140c31ad4e1ba412c91936e1e05d2021-12-02T12:32:55ZThree-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array10.1038/s41598-017-04718-12045-2322https://doaj.org/article/c5c1140c31ad4e1ba412c91936e1e05d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04718-1https://doaj.org/toc/2045-2322Abstract Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments.Ching-Te KuoJong-Yueh WangYu-Fen LinAndrew M. WoBenjamin P. C. ChenHsinyu LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ching-Te Kuo Jong-Yueh Wang Yu-Fen Lin Andrew M. Wo Benjamin P. C. Chen Hsinyu Lee Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
description	Abstract Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments.
format	article
author	Ching-Te Kuo Jong-Yueh Wang Yu-Fen Lin Andrew M. Wo Benjamin P. C. Chen Hsinyu Lee
author_facet	Ching-Te Kuo Jong-Yueh Wang Yu-Fen Lin Andrew M. Wo Benjamin P. C. Chen Hsinyu Lee
author_sort	Ching-Te Kuo
title	Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
title_short	Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
title_full	Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
title_fullStr	Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
title_full_unstemmed	Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array
title_sort	three-dimensional spheroid culture targeting versatile tissue bioassays using a pdms-based hanging drop array
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c5c1140c31ad4e1ba412c91936e1e05d
work_keys_str_mv	AT chingtekuo threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray AT jongyuehwang threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray AT yufenlin threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray AT andrewmwo threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray AT benjaminpcchen threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray AT hsinyulee threedimensionalspheroidculturetargetingversatiletissuebioassaysusingapdmsbasedhangingdroparray
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Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array

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