Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)

Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also an...

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Autores principales:	Dary Luz MENDOZA MEZA, Stephanie SAAVEDRA A.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2013
Materias:	Ulomoides dermestoides beetles metabolites biological assay Chorioallantoic Membrane Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/c5c12cb8a5db48b7afc191e8254700ad
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spelling	oai:doaj.org-article:c5c12cb8a5db48b7afc191e8254700ad2021-11-19T04:12:23ZChemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)0121-40042145-2660https://doaj.org/article/c5c12cb8a5db48b7afc191e8254700ad2013-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/10994https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also anti-inflammatory and immunomodulatory activity of aqueous whole body extract of beetle was reported, it suggests the existence of components with potential pharmacology use. On the other hand, it is necessary to identify those polar and non-polar extracts of U. dermestoides with anti-irritant properties for the membranes and blood vessels, which will be used in subsequence biological test and clinical assays. Objectives: The purpose of this research was to identify the chemical composition of methanolic and hexanic extracts of U. dermestoides, and to assess their anti-irritant capacity. Methods: The extracts were obtained from adult beetles of U. dermestoides. The chemical composition of the extracts was determined by gas chromatography-mass spectrometry (GC-MS) and the anti-irritant effect of each extract was evaluated by means of a modified assay of irritation of the chorioallantoic membrane (CAM) of fertilized chicken eggs (HET-CAM); the results were expressed as irritation index (IR). Results: Six common compounds were identified in both extracts: limonene, myristic, palmitic, estearic, oleic, and linoleic fatty acids. But in the alone methanolic extract were found: 1-pentadecanol, alpha-pinene, beta-phellandrene and alpha-terpinene, whereas in the hexanic extract were found: 2-methyl-p-benzoquinone, 2,4-dihidroxy-1-ethylbenzene, 2,5-dimethylquinone, saturated and unsaturated hydrocarbons and alcohols. The methanolic extract of U. dermestoides showed potential anti-irritant effect in the HET-CAM test (IR = 3.09 ± 0.11), similar to that observed with Nimesulida (IR = 2.05 ± 0.14), a non-steroid anti-inflammatory drug (NSAID) used as positive control for irritation inhibition reaction. The hexanic extract did not show anti-irritant capacity. Conclusions: The results demonstrated the anti-irritant effect of the methanolic extracts of U. dermestoides that could be attributed to compounds with anti-inflammatory activity as oleic acid and limonene.Dary Luz MENDOZA MEZAStephanie SAAVEDRA A.Universidad de AntioquiaarticleUlomoides dermestoidesbeetlesmetabolitesbiological assayChorioallantoic MembraneFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 20, Iss 1 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ulomoides dermestoides beetles metabolites biological assay Chorioallantoic Membrane Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Ulomoides dermestoides beetles metabolites biological assay Chorioallantoic Membrane Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Dary Luz MENDOZA MEZA Stephanie SAAVEDRA A. Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
description	Background: In traditional medicine of Central and South America, the tenebrionid beetle Ulomoides dermestoides is used as an a phrodisiac, for the treatment of inflammatory diseases and cancer. Recently was reported cytotoxic and genotoxic properties of non-polar extract of U. dermestoides; also anti-inflammatory and immunomodulatory activity of aqueous whole body extract of beetle was reported, it suggests the existence of components with potential pharmacology use. On the other hand, it is necessary to identify those polar and non-polar extracts of U. dermestoides with anti-irritant properties for the membranes and blood vessels, which will be used in subsequence biological test and clinical assays. Objectives: The purpose of this research was to identify the chemical composition of methanolic and hexanic extracts of U. dermestoides, and to assess their anti-irritant capacity. Methods: The extracts were obtained from adult beetles of U. dermestoides. The chemical composition of the extracts was determined by gas chromatography-mass spectrometry (GC-MS) and the anti-irritant effect of each extract was evaluated by means of a modified assay of irritation of the chorioallantoic membrane (CAM) of fertilized chicken eggs (HET-CAM); the results were expressed as irritation index (IR). Results: Six common compounds were identified in both extracts: limonene, myristic, palmitic, estearic, oleic, and linoleic fatty acids. But in the alone methanolic extract were found: 1-pentadecanol, alpha-pinene, beta-phellandrene and alpha-terpinene, whereas in the hexanic extract were found: 2-methyl-p-benzoquinone, 2,4-dihidroxy-1-ethylbenzene, 2,5-dimethylquinone, saturated and unsaturated hydrocarbons and alcohols. The methanolic extract of U. dermestoides showed potential anti-irritant effect in the HET-CAM test (IR = 3.09 ± 0.11), similar to that observed with Nimesulida (IR = 2.05 ± 0.14), a non-steroid anti-inflammatory drug (NSAID) used as positive control for irritation inhibition reaction. The hexanic extract did not show anti-irritant capacity. Conclusions: The results demonstrated the anti-irritant effect of the methanolic extracts of U. dermestoides that could be attributed to compounds with anti-inflammatory activity as oleic acid and limonene.
format	article
author	Dary Luz MENDOZA MEZA Stephanie SAAVEDRA A.
author_facet	Dary Luz MENDOZA MEZA Stephanie SAAVEDRA A.
author_sort	Dary Luz MENDOZA MEZA
title	Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
title_short	Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
title_full	Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
title_fullStr	Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
title_full_unstemmed	Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)
title_sort	chemical composition and anti-irritant capacity of whole body extracts of ulomoides dermestoides (coleoptera, tenebrionidae)
publisher	Universidad de Antioquia
publishDate	2013
url	https://doaj.org/article/c5c12cb8a5db48b7afc191e8254700ad
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Chemical composition and anti-irritant capacity of whole body extracts of Ulomoides dermestoides (coleoptera, tenebrionidae)

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