In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone

Shawn H MacVane,1 Seth T Housman,1 David P Nicolau1,21Center for Anti-Infective Research and Development, 2Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USAPurpose: Microdialysis is a valuable technique for studying the distribution of drugs into interstitial fluid, the target s...

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Autores principales:	MacVane SH, Housman ST, Nicolau DP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c5c539a0ae5f4d2184103714b14d7d33
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spelling	oai:doaj.org-article:c5c539a0ae5f4d2184103714b14d7d332021-12-02T04:23:13ZIn vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone1179-1438https://doaj.org/article/c5c539a0ae5f4d2184103714b14d7d332014-06-01T00:00:00Zhttp://www.dovepress.com/in-vitro-microdialysis-membrane-efficiency-of-broad-spectrum-antibioti-a17108https://doaj.org/toc/1179-1438 Shawn H MacVane,1 Seth T Housman,1 David P Nicolau1,21Center for Anti-Infective Research and Development, 2Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USAPurpose: Microdialysis is a valuable technique for studying the distribution of drugs into interstitial fluid, the target site for a pharmacologic effect. Due to incomplete equilibrium, retrodialysis is a method used to correct for relative recovery. The impact of two-drug combinations on probe recovery, however, remains unknown.Methods: In vitro microdialysis was conducted for five antibiotics (avibactam, cefepime, ceftaroline, piperacillin-tazobactam, and vancomycin), representing three empiric antimicrobial regimens, to assess the impact of two-drug combinations on probe recovery. Recoveries were compared between single and two-drug treatments.Results: Recoveries by gain and loss were linear with their molecular weight. During all gain experiments, recoveries were similar when tested alone or in combination with another antibiotic. Unacceptable differences in recovery by loss were observed for cefepime in the presence of vancomycin (-21%) and vancomycin in the presence of piperacillin-tazobactam (-22%).Conclusion: Differences among in vitro recovery by loss suggest two-drug combinations may impact dialysate recovery during in vivo retrodialysis procedures, particularly when larger molecular weight drugs (ie, vancomycin) are involved. Importantly, there were no differences during gain experiments. In vitro studies, as performed here, should be conducted for each potential two-drug combination, prior to their combined use for in vivo retrodialysis.Keywords: microdialysis, retrodialysis, combination therapyMacVane SHHousman STNicolau DPDove Medical PressarticleTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol 2014, Iss default, Pp 97-101 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 MacVane SH Housman ST Nicolau DP In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
description	Shawn H MacVane,1 Seth T Housman,1 David P Nicolau1,21Center for Anti-Infective Research and Development, 2Division of Infectious Diseases, Hartford Hospital, Hartford, CT, USAPurpose: Microdialysis is a valuable technique for studying the distribution of drugs into interstitial fluid, the target site for a pharmacologic effect. Due to incomplete equilibrium, retrodialysis is a method used to correct for relative recovery. The impact of two-drug combinations on probe recovery, however, remains unknown.Methods: In vitro microdialysis was conducted for five antibiotics (avibactam, cefepime, ceftaroline, piperacillin-tazobactam, and vancomycin), representing three empiric antimicrobial regimens, to assess the impact of two-drug combinations on probe recovery. Recoveries were compared between single and two-drug treatments.Results: Recoveries by gain and loss were linear with their molecular weight. During all gain experiments, recoveries were similar when tested alone or in combination with another antibiotic. Unacceptable differences in recovery by loss were observed for cefepime in the presence of vancomycin (-21%) and vancomycin in the presence of piperacillin-tazobactam (-22%).Conclusion: Differences among in vitro recovery by loss suggest two-drug combinations may impact dialysate recovery during in vivo retrodialysis procedures, particularly when larger molecular weight drugs (ie, vancomycin) are involved. Importantly, there were no differences during gain experiments. In vitro studies, as performed here, should be conducted for each potential two-drug combination, prior to their combined use for in vivo retrodialysis.Keywords: microdialysis, retrodialysis, combination therapy
format	article
author	MacVane SH Housman ST Nicolau DP
author_facet	MacVane SH Housman ST Nicolau DP
author_sort	MacVane SH
title	In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
title_short	In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
title_full	In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
title_fullStr	In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
title_full_unstemmed	In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
title_sort	in vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/c5c539a0ae5f4d2184103714b14d7d33
work_keys_str_mv	AT macvanesh invitromicrodialysismembraneefficiencyofbroadspectrumantibioticsincombinationandalone AT housmanst invitromicrodialysismembraneefficiencyofbroadspectrumantibioticsincombinationandalone AT nicolaudp invitromicrodialysismembraneefficiencyofbroadspectrumantibioticsincombinationandalone
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In vitro microdialysis membrane efficiency of broad-spectrum antibiotics in combination and alone

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