Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer

Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus...

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Autores principales: FITMAWATI FITMAWATI, ROSLINA FAUZIAH, IBNA HAYATI, NERY SOFIYANTI, EIICHI INOUE, DEDEN DERAJAT MATRA
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spelling oai:doaj.org-article:c5c7bc793a6840ab991ec843db33e5d72021-11-16T13:56:34ZPhylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer1412-033X2085-472210.13057/biodiv/d180322https://doaj.org/article/c5c7bc793a6840ab991ec843db33e5d72017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2021https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus is therefore as a potential germplasm source in Sumatra. The aim of this study was to analyze and compare the nucleotide sequences of Mangifera in the central region of Sumatra based on gene loci of trnL-F intergenic spacer. The initial step of research was conducted by isolating DNA from leaves sample of Mangifera based on CTAB method. Sequences of gene loci of trnL-F were analyzed with Maximum Parsimony and Neighbour Joining methods through PAUP* Program version 4.0b10. From the results of sequences analysis with Maximum Parsimony method, it was obtained a monophyletic group of Mangifera consisted of two main clades which separated M. kemanga from five other species of Mangifera, i.e. M. foetida, M. odorata, M. laurina and Mangifera sp. Parsimony analysis also revealed that the common ancestor of M. kemanga is the first species appeared in the central region of Sumatra. The result of Neighbour Joining analysis showed M. laurina had the longest genetic distance among examined species and might be assumed as the most primitive species of Mangifera. Mangifera laurina and M. odorata were closely related as well as M. foetida and M. odorata. The results of research also revealed and supported the genus Mangifera as a monophyletic group.FITMAWATI FITMAWATIROSLINA FAUZIAHIBNA HAYATINERY SOFIYANTIEIICHI INOUEDEDEN DERAJAT MATRAMBI & UNS Soloarticlecentral region of sumatracpdnamangiferaphylogenetic analysistrnl-f intergenic spacer sequencesBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1035-1040 (2017)
institution DOAJ
collection DOAJ
language EN
topic central region of sumatra
cpdna
mangifera
phylogenetic analysis
trnl-f intergenic spacer sequences
Biology (General)
QH301-705.5
spellingShingle central region of sumatra
cpdna
mangifera
phylogenetic analysis
trnl-f intergenic spacer sequences
Biology (General)
QH301-705.5
FITMAWATI FITMAWATI
ROSLINA FAUZIAH
IBNA HAYATI
NERY SOFIYANTI
EIICHI INOUE
DEDEN DERAJAT MATRA
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
description Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus is therefore as a potential germplasm source in Sumatra. The aim of this study was to analyze and compare the nucleotide sequences of Mangifera in the central region of Sumatra based on gene loci of trnL-F intergenic spacer. The initial step of research was conducted by isolating DNA from leaves sample of Mangifera based on CTAB method. Sequences of gene loci of trnL-F were analyzed with Maximum Parsimony and Neighbour Joining methods through PAUP* Program version 4.0b10. From the results of sequences analysis with Maximum Parsimony method, it was obtained a monophyletic group of Mangifera consisted of two main clades which separated M. kemanga from five other species of Mangifera, i.e. M. foetida, M. odorata, M. laurina and Mangifera sp. Parsimony analysis also revealed that the common ancestor of M. kemanga is the first species appeared in the central region of Sumatra. The result of Neighbour Joining analysis showed M. laurina had the longest genetic distance among examined species and might be assumed as the most primitive species of Mangifera. Mangifera laurina and M. odorata were closely related as well as M. foetida and M. odorata. The results of research also revealed and supported the genus Mangifera as a monophyletic group.
format article
author FITMAWATI FITMAWATI
ROSLINA FAUZIAH
IBNA HAYATI
NERY SOFIYANTI
EIICHI INOUE
DEDEN DERAJAT MATRA
author_facet FITMAWATI FITMAWATI
ROSLINA FAUZIAH
IBNA HAYATI
NERY SOFIYANTI
EIICHI INOUE
DEDEN DERAJAT MATRA
author_sort FITMAWATI FITMAWATI
title Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
title_short Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
title_full Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
title_fullStr Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
title_full_unstemmed Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
title_sort phylogenetic analysis of mangifera from central region of sumatra using trnl-f intergenic spacer
publisher MBI & UNS Solo
publishDate 2017
url https://doaj.org/article/c5c7bc793a6840ab991ec843db33e5d7
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