Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer
Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus...
Guardado en:
Autores principales: | , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
MBI & UNS Solo
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/c5c7bc793a6840ab991ec843db33e5d7 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:c5c7bc793a6840ab991ec843db33e5d7 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:c5c7bc793a6840ab991ec843db33e5d72021-11-16T13:56:34ZPhylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer1412-033X2085-472210.13057/biodiv/d180322https://doaj.org/article/c5c7bc793a6840ab991ec843db33e5d72017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2021https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus is therefore as a potential germplasm source in Sumatra. The aim of this study was to analyze and compare the nucleotide sequences of Mangifera in the central region of Sumatra based on gene loci of trnL-F intergenic spacer. The initial step of research was conducted by isolating DNA from leaves sample of Mangifera based on CTAB method. Sequences of gene loci of trnL-F were analyzed with Maximum Parsimony and Neighbour Joining methods through PAUP* Program version 4.0b10. From the results of sequences analysis with Maximum Parsimony method, it was obtained a monophyletic group of Mangifera consisted of two main clades which separated M. kemanga from five other species of Mangifera, i.e. M. foetida, M. odorata, M. laurina and Mangifera sp. Parsimony analysis also revealed that the common ancestor of M. kemanga is the first species appeared in the central region of Sumatra. The result of Neighbour Joining analysis showed M. laurina had the longest genetic distance among examined species and might be assumed as the most primitive species of Mangifera. Mangifera laurina and M. odorata were closely related as well as M. foetida and M. odorata. The results of research also revealed and supported the genus Mangifera as a monophyletic group.FITMAWATI FITMAWATIROSLINA FAUZIAHIBNA HAYATINERY SOFIYANTIEIICHI INOUEDEDEN DERAJAT MATRAMBI & UNS Soloarticlecentral region of sumatracpdnamangiferaphylogenetic analysistrnl-f intergenic spacer sequencesBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1035-1040 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
central region of sumatra cpdna mangifera phylogenetic analysis trnl-f intergenic spacer sequences Biology (General) QH301-705.5 |
spellingShingle |
central region of sumatra cpdna mangifera phylogenetic analysis trnl-f intergenic spacer sequences Biology (General) QH301-705.5 FITMAWATI FITMAWATI ROSLINA FAUZIAH IBNA HAYATI NERY SOFIYANTI EIICHI INOUE DEDEN DERAJAT MATRA Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
description |
Fitmawati, Fauziah R, Hayati I, Sofiyanti N, Inoue E, Matra DD. 2017. Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer. Biodiversitas 18: 1035-1040. Mangifera from Central Sumatra has an unique ability to adapt to the high rainfall regions. This genus is therefore as a potential germplasm source in Sumatra. The aim of this study was to analyze and compare the nucleotide sequences of Mangifera in the central region of Sumatra based on gene loci of trnL-F intergenic spacer. The initial step of research was conducted by isolating DNA from leaves sample of Mangifera based on CTAB method. Sequences of gene loci of trnL-F were analyzed with Maximum Parsimony and Neighbour Joining methods through PAUP* Program version 4.0b10. From the results of sequences analysis with Maximum Parsimony method, it was obtained a monophyletic group of Mangifera consisted of two main clades which separated M. kemanga from five other species of Mangifera, i.e. M. foetida, M. odorata, M. laurina and Mangifera sp. Parsimony analysis also revealed that the common ancestor of M. kemanga is the first species appeared in the central region of Sumatra. The result of Neighbour Joining analysis showed M. laurina had the longest genetic distance among examined species and might be assumed as the most primitive species of Mangifera. Mangifera laurina and M. odorata were closely related as well as M. foetida and M. odorata. The results of research also revealed and supported the genus Mangifera as a monophyletic group. |
format |
article |
author |
FITMAWATI FITMAWATI ROSLINA FAUZIAH IBNA HAYATI NERY SOFIYANTI EIICHI INOUE DEDEN DERAJAT MATRA |
author_facet |
FITMAWATI FITMAWATI ROSLINA FAUZIAH IBNA HAYATI NERY SOFIYANTI EIICHI INOUE DEDEN DERAJAT MATRA |
author_sort |
FITMAWATI FITMAWATI |
title |
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
title_short |
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
title_full |
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
title_fullStr |
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
title_full_unstemmed |
Phylogenetic analysis of Mangifera from central region of Sumatra using trnL-F intergenic spacer |
title_sort |
phylogenetic analysis of mangifera from central region of sumatra using trnl-f intergenic spacer |
publisher |
MBI & UNS Solo |
publishDate |
2017 |
url |
https://doaj.org/article/c5c7bc793a6840ab991ec843db33e5d7 |
work_keys_str_mv |
AT fitmawatifitmawati phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer AT roslinafauziah phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer AT ibnahayati phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer AT nerysofiyanti phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer AT eiichiinoue phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer AT dedenderajatmatra phylogeneticanalysisofmangiferafromcentralregionofsumatrausingtrnlfintergenicspacer |
_version_ |
1718426505231466496 |