Targeted metabolomic profiling in rat tissues reveals sex differences

Abstract Sex differences affect several diseases and are organ-and parameter-specific. In humans and animals, sex differences also influence the metabolism and homeostasis of amino acids and fatty acids, which are linked to the onset of diseases. Thus, the use of targeted metabolite profiles in tiss...

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Autores principales:	Margherita Ruoppolo, Marianna Caterino, Lucia Albano, Rita Pecce, Maria Grazia Di Girolamo, Daniela Crisci, Michele Costanzo, Luigi Milella, Flavia Franconi, Ilaria Campesi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5ca532ade6847b1b72e16dabf62d7a7
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spelling	oai:doaj.org-article:c5ca532ade6847b1b72e16dabf62d7a72021-12-02T15:08:52ZTargeted metabolomic profiling in rat tissues reveals sex differences10.1038/s41598-018-22869-72045-2322https://doaj.org/article/c5ca532ade6847b1b72e16dabf62d7a72018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22869-7https://doaj.org/toc/2045-2322Abstract Sex differences affect several diseases and are organ-and parameter-specific. In humans and animals, sex differences also influence the metabolism and homeostasis of amino acids and fatty acids, which are linked to the onset of diseases. Thus, the use of targeted metabolite profiles in tissues represents a powerful approach to examine the intermediary metabolism and evidence for any sex differences. To clarify the sex-specific activities of liver, heart and kidney tissues, we used targeted metabolomics, linear discriminant analysis (LDA), principal component analysis (PCA), cluster analysis and linear correlation models to evaluate sex and organ-specific differences in amino acids, free carnitine and acylcarnitine levels in male and female Sprague-Dawley rats. Several intra-sex differences affect tissues, indicating that metabolite profiles in rat hearts, livers and kidneys are organ-dependent. Amino acids and carnitine levels in rat hearts, livers and kidneys are affected by sex: male and female hearts show the greatest sexual dimorphism, both qualitatively and quantitatively. Finally, multivariate analysis confirmed the influence of sex on the metabolomics profiling. Our data demonstrate that the metabolomics approach together with a multivariate approach can capture the dynamics of physiological and pathological states, which are essential for explaining the basis of the sex differences observed in physiological and pathological conditions.Margherita RuoppoloMarianna CaterinoLucia AlbanoRita PecceMaria Grazia Di GirolamoDaniela CrisciMichele CostanzoLuigi MilellaFlavia FranconiIlaria CampesiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margherita Ruoppolo Marianna Caterino Lucia Albano Rita Pecce Maria Grazia Di Girolamo Daniela Crisci Michele Costanzo Luigi Milella Flavia Franconi Ilaria Campesi Targeted metabolomic profiling in rat tissues reveals sex differences
description	Abstract Sex differences affect several diseases and are organ-and parameter-specific. In humans and animals, sex differences also influence the metabolism and homeostasis of amino acids and fatty acids, which are linked to the onset of diseases. Thus, the use of targeted metabolite profiles in tissues represents a powerful approach to examine the intermediary metabolism and evidence for any sex differences. To clarify the sex-specific activities of liver, heart and kidney tissues, we used targeted metabolomics, linear discriminant analysis (LDA), principal component analysis (PCA), cluster analysis and linear correlation models to evaluate sex and organ-specific differences in amino acids, free carnitine and acylcarnitine levels in male and female Sprague-Dawley rats. Several intra-sex differences affect tissues, indicating that metabolite profiles in rat hearts, livers and kidneys are organ-dependent. Amino acids and carnitine levels in rat hearts, livers and kidneys are affected by sex: male and female hearts show the greatest sexual dimorphism, both qualitatively and quantitatively. Finally, multivariate analysis confirmed the influence of sex on the metabolomics profiling. Our data demonstrate that the metabolomics approach together with a multivariate approach can capture the dynamics of physiological and pathological states, which are essential for explaining the basis of the sex differences observed in physiological and pathological conditions.
format	article
author	Margherita Ruoppolo Marianna Caterino Lucia Albano Rita Pecce Maria Grazia Di Girolamo Daniela Crisci Michele Costanzo Luigi Milella Flavia Franconi Ilaria Campesi
author_facet	Margherita Ruoppolo Marianna Caterino Lucia Albano Rita Pecce Maria Grazia Di Girolamo Daniela Crisci Michele Costanzo Luigi Milella Flavia Franconi Ilaria Campesi
author_sort	Margherita Ruoppolo
title	Targeted metabolomic profiling in rat tissues reveals sex differences
title_short	Targeted metabolomic profiling in rat tissues reveals sex differences
title_full	Targeted metabolomic profiling in rat tissues reveals sex differences
title_fullStr	Targeted metabolomic profiling in rat tissues reveals sex differences
title_full_unstemmed	Targeted metabolomic profiling in rat tissues reveals sex differences
title_sort	targeted metabolomic profiling in rat tissues reveals sex differences
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c5ca532ade6847b1b72e16dabf62d7a7
work_keys_str_mv	AT margheritaruoppolo targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT mariannacaterino targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT luciaalbano targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT ritapecce targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT mariagraziadigirolamo targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT danielacrisci targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT michelecostanzo targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT luigimilella targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT flaviafranconi targetedmetabolomicprofilinginrattissuesrevealssexdifferences AT ilariacampesi targetedmetabolomicprofilinginrattissuesrevealssexdifferences
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Targeted metabolomic profiling in rat tissues reveals sex differences

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