Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats

The Mela Rosa dei Monti Sibillini is an ancient apple variety cultivated by Romans in the foothills of the Sibillini Mountains, central Italy, showing potential as a source of nutraceuticals. The purpose of this study was to evaluate the protective effects of the hydroalcoholic extracts from the pee...

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Autores principales:	Hasan Yousefi-Manesh, Ahmad Reza Dehpour, Seyed Mohammad Nabavi, Malihe Khayatkashani, Mohammad Hossein Asgardoon, Mohamad Hasan Derakhshan, Sahand Adib Moradi, Mohammad Sheibani, Seyed Mohammad Tavangar, Samira Shirooie, Joice Guileine Nkuimi Wandjou, Giovanni Caprioli, Stefania Sut, Stefano Dall’Acqua, Filippo Maggi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Mela Rosa dei Monti Sibillini apple extract ischemic stroke inflammation anti-oxidative stress caspase-3 Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/c5db2004ee7141a38199890044bdd64a
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spelling	oai:doaj.org-article:c5db2004ee7141a38199890044bdd64a2021-11-25T18:39:23ZTherapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats10.3390/ph141111061424-8247https://doaj.org/article/c5db2004ee7141a38199890044bdd64a2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1106https://doaj.org/toc/1424-8247The Mela Rosa dei Monti Sibillini is an ancient apple variety cultivated by Romans in the foothills of the Sibillini Mountains, central Italy, showing potential as a source of nutraceuticals. The purpose of this study was to evaluate the protective effects of the hydroalcoholic extracts from the peel (APE) and pulp (APP) of this fruit in an animal model of transient global ischemia. Chemical constituents were analyzed by liquid chromatography–mass spectrometry (LC-DAD-MS<sup>n</sup>) indicating several polyphenols such as B-type procyanidins, quercetin derivatives and hydroxycinnamic acids as the main bioactive components. Acute pre-treatment of extracts (30 mg/kg, i.p.) significantly decreased the brain levels of the pro-inflammatory cytokines IL-1β (<i>p</i> < 0.01) and TNF-α (<i>p</i> < 0.001 and <i>p</i> < 0.01 for APE and APP, respectively), the expression of caspase-3 (<i>p</i> < 0.01, For APE) and MDA (<i>p</i> < 0.05), a lipid peroxidation biomarker in rats. Both extracts restricted the pathological changes of the brain induced by ischemic stroke in hematoxylin and eosin assay. Moreover, they improved the scores of behavioral tests in grid-walking and modified neurological severity scores (mNSS) tests. In conclusion, these results proved this ancient Italian apple is a source of nutraceuticals able to protect/prevent damage from brain ischemia.Hasan Yousefi-ManeshAhmad Reza DehpourSeyed Mohammad NabaviMalihe KhayatkashaniMohammad Hossein AsgardoonMohamad Hasan DerakhshanSahand Adib MoradiMohammad SheibaniSeyed Mohammad TavangarSamira ShirooieJoice Guileine Nkuimi WandjouGiovanni CaprioliStefania SutStefano Dall’AcquaFilippo MaggiMDPI AGarticleMela Rosa dei Monti Sibilliniapple extractischemic strokeinflammationanti-oxidative stresscaspase-3MedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1106, p 1106 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mela Rosa dei Monti Sibillini apple extract ischemic stroke inflammation anti-oxidative stress caspase-3 Medicine R Pharmacy and materia medica RS1-441
spellingShingle	Mela Rosa dei Monti Sibillini apple extract ischemic stroke inflammation anti-oxidative stress caspase-3 Medicine R Pharmacy and materia medica RS1-441 Hasan Yousefi-Manesh Ahmad Reza Dehpour Seyed Mohammad Nabavi Malihe Khayatkashani Mohammad Hossein Asgardoon Mohamad Hasan Derakhshan Sahand Adib Moradi Mohammad Sheibani Seyed Mohammad Tavangar Samira Shirooie Joice Guileine Nkuimi Wandjou Giovanni Caprioli Stefania Sut Stefano Dall’Acqua Filippo Maggi Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
description	The Mela Rosa dei Monti Sibillini is an ancient apple variety cultivated by Romans in the foothills of the Sibillini Mountains, central Italy, showing potential as a source of nutraceuticals. The purpose of this study was to evaluate the protective effects of the hydroalcoholic extracts from the peel (APE) and pulp (APP) of this fruit in an animal model of transient global ischemia. Chemical constituents were analyzed by liquid chromatography–mass spectrometry (LC-DAD-MS<sup>n</sup>) indicating several polyphenols such as B-type procyanidins, quercetin derivatives and hydroxycinnamic acids as the main bioactive components. Acute pre-treatment of extracts (30 mg/kg, i.p.) significantly decreased the brain levels of the pro-inflammatory cytokines IL-1β (<i>p</i> < 0.01) and TNF-α (<i>p</i> < 0.001 and <i>p</i> < 0.01 for APE and APP, respectively), the expression of caspase-3 (<i>p</i> < 0.01, For APE) and MDA (<i>p</i> < 0.05), a lipid peroxidation biomarker in rats. Both extracts restricted the pathological changes of the brain induced by ischemic stroke in hematoxylin and eosin assay. Moreover, they improved the scores of behavioral tests in grid-walking and modified neurological severity scores (mNSS) tests. In conclusion, these results proved this ancient Italian apple is a source of nutraceuticals able to protect/prevent damage from brain ischemia.
format	article
author	Hasan Yousefi-Manesh Ahmad Reza Dehpour Seyed Mohammad Nabavi Malihe Khayatkashani Mohammad Hossein Asgardoon Mohamad Hasan Derakhshan Sahand Adib Moradi Mohammad Sheibani Seyed Mohammad Tavangar Samira Shirooie Joice Guileine Nkuimi Wandjou Giovanni Caprioli Stefania Sut Stefano Dall’Acqua Filippo Maggi
author_facet	Hasan Yousefi-Manesh Ahmad Reza Dehpour Seyed Mohammad Nabavi Malihe Khayatkashani Mohammad Hossein Asgardoon Mohamad Hasan Derakhshan Sahand Adib Moradi Mohammad Sheibani Seyed Mohammad Tavangar Samira Shirooie Joice Guileine Nkuimi Wandjou Giovanni Caprioli Stefania Sut Stefano Dall’Acqua Filippo Maggi
author_sort	Hasan Yousefi-Manesh
title	Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
title_short	Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
title_full	Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
title_fullStr	Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
title_full_unstemmed	Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats
title_sort	therapeutic effects of hydroalcoholic extracts from the ancient apple mela rosa dei monti sibillini in transient global ischemia in rats
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c5db2004ee7141a38199890044bdd64a
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Therapeutic Effects of Hydroalcoholic Extracts from the Ancient Apple Mela Rosa dei Monti Sibillini in Transient Global Ischemia in Rats

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