Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism

Abstract In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology a...

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Autores principales:	Alexander Jonsson, Anders Hedin, Malin Müller, Oskar Skog, Olle Korsgren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5dfec5daa10486485c43981097e6c15
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spelling	oai:doaj.org-article:c5dfec5daa10486485c43981097e6c152021-12-02T11:57:56ZTranscriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism10.1038/s41598-020-79313-y2045-2322https://doaj.org/article/c5dfec5daa10486485c43981097e6c152020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79313-yhttps://doaj.org/toc/2045-2322Abstract In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology and gene expression. In this study, islets and exocrine pancreatic tissue were acquired from organ donors with normoglycemia or impaired glucose metabolism (IGM) immediately after islet isolation. Following single-cell dissociation, primary islet- and exocrine MVECs were obtained through fluorescence-activated cell sorting (FACS) and transcriptional profiles were generated using AmpliSeq. Multiple gene sets involved in general vascular development and extracellular matrix remodeling were enriched in islet MVEC. In exocrine MVEC samples, multiple enriched gene sets that relate to biosynthesis and biomolecule catabolism were found. No statistically significant enrichment was found in gene sets related to autophagy or endoplasmic reticulum (ER) stress. Although ample differences were found between islet- and exocrine tissue endothelial cells, no differences could be observed between normoglycemic donors and donors with IGM at gene or gene set level. Our data is consistent with active angiogenesis and vascular remodeling in human islets and support the notion of ongoing endocrine pancreas tissue repair and regeneration even in the adult human.Alexander JonssonAnders HedinMalin MüllerOskar SkogOlle KorsgrenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander Jonsson Anders Hedin Malin Müller Oskar Skog Olle Korsgren Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
description	Abstract In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology and gene expression. In this study, islets and exocrine pancreatic tissue were acquired from organ donors with normoglycemia or impaired glucose metabolism (IGM) immediately after islet isolation. Following single-cell dissociation, primary islet- and exocrine MVECs were obtained through fluorescence-activated cell sorting (FACS) and transcriptional profiles were generated using AmpliSeq. Multiple gene sets involved in general vascular development and extracellular matrix remodeling were enriched in islet MVEC. In exocrine MVEC samples, multiple enriched gene sets that relate to biosynthesis and biomolecule catabolism were found. No statistically significant enrichment was found in gene sets related to autophagy or endoplasmic reticulum (ER) stress. Although ample differences were found between islet- and exocrine tissue endothelial cells, no differences could be observed between normoglycemic donors and donors with IGM at gene or gene set level. Our data is consistent with active angiogenesis and vascular remodeling in human islets and support the notion of ongoing endocrine pancreas tissue repair and regeneration even in the adult human.
format	article
author	Alexander Jonsson Anders Hedin Malin Müller Oskar Skog Olle Korsgren
author_facet	Alexander Jonsson Anders Hedin Malin Müller Oskar Skog Olle Korsgren
author_sort	Alexander Jonsson
title	Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
title_short	Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
title_full	Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
title_fullStr	Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
title_full_unstemmed	Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
title_sort	transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c5dfec5daa10486485c43981097e6c15
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Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism

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