NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli

Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase Al...

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Autores principales:	Valentin F. Reichle, Dimitar P. Petrov, Verena Weber, Kirsten Jung, Stefanie Kellner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c5e018036abe4648a274d3e1f770c4fd
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spelling	oai:doaj.org-article:c5e018036abe4648a274d3e1f770c4fd2021-12-02T14:39:02ZNAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli10.1038/s41467-019-13565-92041-1723https://doaj.org/article/c5e018036abe4648a274d3e1f770c4fd2019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13565-9https://doaj.org/toc/2041-1723Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase AlkB in vivo.Valentin F. ReichleDimitar P. PetrovVerena WeberKirsten JungStefanie KellnerNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Valentin F. Reichle Dimitar P. Petrov Verena Weber Kirsten Jung Stefanie Kellner NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
description	Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase AlkB in vivo.
format	article
author	Valentin F. Reichle Dimitar P. Petrov Verena Weber Kirsten Jung Stefanie Kellner
author_facet	Valentin F. Reichle Dimitar P. Petrov Verena Weber Kirsten Jung Stefanie Kellner
author_sort	Valentin F. Reichle
title	NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
title_short	NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
title_full	NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
title_fullStr	NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
title_full_unstemmed	NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli
title_sort	nail-ms reveals the repair of 2-methylthiocytidine by alkb in e. coli
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c5e018036abe4648a274d3e1f770c4fd
work_keys_str_mv	AT valentinfreichle nailmsrevealstherepairof2methylthiocytidinebyalkbinecoli AT dimitarppetrov nailmsrevealstherepairof2methylthiocytidinebyalkbinecoli AT verenaweber nailmsrevealstherepairof2methylthiocytidinebyalkbinecoli AT kirstenjung nailmsrevealstherepairof2methylthiocytidinebyalkbinecoli AT stefaniekellner nailmsrevealstherepairof2methylthiocytidinebyalkbinecoli
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NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli

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