Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance

Abstract Resistance to root-knot nematode was introgressed into cultivated peanut Arachis hypogaea from a wild peanut relative, A. cardenasii and previously mapped to chromosome A09. The highly resistant recombinant inbred RIL 46 and moderately resistant RIL 48 were selected from a population with c...

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Autores principales:	Josh Clevenger, Ye Chu, Larissa Arrais Guimaraes, Thiago Maia, David Bertioli, Soraya Leal-Bertioli, Patricia Timper, C. Corley Holbrook, Peggy Ozias-Akins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5e0267d86eb401fa2e28680af635f46
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spelling	oai:doaj.org-article:c5e0267d86eb401fa2e28680af635f462021-12-02T11:52:24ZGene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance10.1038/s41598-017-00971-62045-2322https://doaj.org/article/c5e0267d86eb401fa2e28680af635f462017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00971-6https://doaj.org/toc/2045-2322Abstract Resistance to root-knot nematode was introgressed into cultivated peanut Arachis hypogaea from a wild peanut relative, A. cardenasii and previously mapped to chromosome A09. The highly resistant recombinant inbred RIL 46 and moderately resistant RIL 48 were selected from a population with cv. Gregory (susceptible) and Tifguard (resistant) as female and male parents, respectively. RNA-seq analysis was performed on these four genotypes using root tissue harvested from root-knot nematode infected plants at 0, 3, 7 days after inoculation. Differential gene expression analysis provides evidence that root-knot nematodes modulate biological pathways involved in plant hormone, defense, cell signaling, cytoskeleton and cell wall metabolism in a susceptible reaction. Corresponding to resistance reaction, an effector-induced-immune response mediated by an R-gene was identified in Tifguard. Mapping of the introgressed region indicated that 92% of linkage group A09 was of A. cardenasii origin in Tifguard. RIL46 and RIL 48 possessed 3.6% and 83.5% of the introgression on A09, respectively. Within the small introgressed region carried by RIL 46, a constitutively expressed TIR-NBS-LRR gene was identified as the candidate for nematode resistance. Potential defense responsive pathways include effector endocytosis through clathrin-coated vesicle trafficking, defense signaling through membrane lipid metabolism and mucilage production.Josh ClevengerYe ChuLarissa Arrais GuimaraesThiago MaiaDavid BertioliSoraya Leal-BertioliPatricia TimperC. Corley HolbrookPeggy Ozias-AkinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Josh Clevenger Ye Chu Larissa Arrais Guimaraes Thiago Maia David Bertioli Soraya Leal-Bertioli Patricia Timper C. Corley Holbrook Peggy Ozias-Akins Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
description	Abstract Resistance to root-knot nematode was introgressed into cultivated peanut Arachis hypogaea from a wild peanut relative, A. cardenasii and previously mapped to chromosome A09. The highly resistant recombinant inbred RIL 46 and moderately resistant RIL 48 were selected from a population with cv. Gregory (susceptible) and Tifguard (resistant) as female and male parents, respectively. RNA-seq analysis was performed on these four genotypes using root tissue harvested from root-knot nematode infected plants at 0, 3, 7 days after inoculation. Differential gene expression analysis provides evidence that root-knot nematodes modulate biological pathways involved in plant hormone, defense, cell signaling, cytoskeleton and cell wall metabolism in a susceptible reaction. Corresponding to resistance reaction, an effector-induced-immune response mediated by an R-gene was identified in Tifguard. Mapping of the introgressed region indicated that 92% of linkage group A09 was of A. cardenasii origin in Tifguard. RIL46 and RIL 48 possessed 3.6% and 83.5% of the introgression on A09, respectively. Within the small introgressed region carried by RIL 46, a constitutively expressed TIR-NBS-LRR gene was identified as the candidate for nematode resistance. Potential defense responsive pathways include effector endocytosis through clathrin-coated vesicle trafficking, defense signaling through membrane lipid metabolism and mucilage production.
format	article
author	Josh Clevenger Ye Chu Larissa Arrais Guimaraes Thiago Maia David Bertioli Soraya Leal-Bertioli Patricia Timper C. Corley Holbrook Peggy Ozias-Akins
author_facet	Josh Clevenger Ye Chu Larissa Arrais Guimaraes Thiago Maia David Bertioli Soraya Leal-Bertioli Patricia Timper C. Corley Holbrook Peggy Ozias-Akins
author_sort	Josh Clevenger
title	Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
title_short	Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
title_full	Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
title_fullStr	Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
title_full_unstemmed	Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance
title_sort	gene expression profiling describes the genetic regulation of meloidogyne arenaria resistance in arachis hypogaea and reveals a candidate gene for resistance
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c5e0267d86eb401fa2e28680af635f46
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Gene expression profiling describes the genetic regulation of Meloidogyne arenaria resistance in Arachis hypogaea and reveals a candidate gene for resistance

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