Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).

Elucidating the mechanisms underlying human pain sensation requires the establishment of an in vitro model of pain reception comprising human cells expressing pain-sensing receptors and function properly as neurons. Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells and a promising can...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Arimura, Yutaka Shindo, Ryu Yamanaka, Mai Mochizuki, Kohji Hotta, Taka Nakahara, Etsuro Ito, Tohru Yoshioka, Kotaro Oka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c5e2ffe1b4df40fcacaeaaf238a7296f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c5e2ffe1b4df40fcacaeaaf238a7296f
record_format	dspace
spelling	oai:doaj.org-article:c5e2ffe1b4df40fcacaeaaf238a7296f2021-11-25T06:19:18ZPeripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).1932-620310.1371/journal.pone.0251356https://doaj.org/article/c5e2ffe1b4df40fcacaeaaf238a7296f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251356https://doaj.org/toc/1932-6203Elucidating the mechanisms underlying human pain sensation requires the establishment of an in vitro model of pain reception comprising human cells expressing pain-sensing receptors and function properly as neurons. Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells and a promising candidate for producing human neuronal cells, however, the functional properties of differentiated hDPSCs have not yet been fully characterized. In this study, we demonstrated neuronal differentiation of hDPSCs via both their expression of neuronal marker proteins and their neuronal function examined using Ca2+ imaging. Moreover, to confirm the ability of nociception, Ca2+ responses in differentiated hDPSCs were compared to those of rat dorsal root ganglion (DRG) neurons. Those cells showed similar responses to glutamate, ATP and agonists of transient receptor potential (TRP) channels. Since TRP channels are implicated in nociception, differentiated hDPSCs provide a useful in vitro model of human peripheral neuron response to stimuli interpreted as pain.Yuki ArimuraYutaka ShindoRyu YamanakaMai MochizukiKohji HottaTaka NakaharaEtsuro ItoTohru YoshiokaKotaro OkaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251356 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Arimura Yutaka Shindo Ryu Yamanaka Mai Mochizuki Kohji Hotta Taka Nakahara Etsuro Ito Tohru Yoshioka Kotaro Oka Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
description	Elucidating the mechanisms underlying human pain sensation requires the establishment of an in vitro model of pain reception comprising human cells expressing pain-sensing receptors and function properly as neurons. Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells and a promising candidate for producing human neuronal cells, however, the functional properties of differentiated hDPSCs have not yet been fully characterized. In this study, we demonstrated neuronal differentiation of hDPSCs via both their expression of neuronal marker proteins and their neuronal function examined using Ca2+ imaging. Moreover, to confirm the ability of nociception, Ca2+ responses in differentiated hDPSCs were compared to those of rat dorsal root ganglion (DRG) neurons. Those cells showed similar responses to glutamate, ATP and agonists of transient receptor potential (TRP) channels. Since TRP channels are implicated in nociception, differentiated hDPSCs provide a useful in vitro model of human peripheral neuron response to stimuli interpreted as pain.
format	article
author	Yuki Arimura Yutaka Shindo Ryu Yamanaka Mai Mochizuki Kohji Hotta Taka Nakahara Etsuro Ito Tohru Yoshioka Kotaro Oka
author_facet	Yuki Arimura Yutaka Shindo Ryu Yamanaka Mai Mochizuki Kohji Hotta Taka Nakahara Etsuro Ito Tohru Yoshioka Kotaro Oka
author_sort	Yuki Arimura
title	Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
title_short	Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
title_full	Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
title_fullStr	Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
title_full_unstemmed	Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).
title_sort	peripheral-neuron-like properties of differentiated human dental pulp stem cells (hdpscs).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c5e2ffe1b4df40fcacaeaaf238a7296f
work_keys_str_mv	AT yukiarimura peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT yutakashindo peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT ryuyamanaka peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT maimochizuki peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT kohjihotta peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT takanakahara peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT etsuroito peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT tohruyoshioka peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs AT kotarooka peripheralneuronlikepropertiesofdifferentiatedhumandentalpulpstemcellshdpscs
_version_	1718413890556002304

Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs).

Ejemplares similares