Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases

The aim of this study was to evaluate production of cytokines by the blood immune cells in patients of different age groups with invasive non-specified type mammary ductal carcinoma, with or without metastases. Production of cytokines by periphery blood immunocompetent cells, either spontaneous and...

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Autores principales:	K. I. Davletova, E. S. Mikhaylova, N. A. Varaksin, L. P. Zhurakovsky, A. V. Proskura, S. V. Sidorov, A. I. Autenshlyus
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	cytokines blood immune cells polyclonal activators breast cancer invasive ductal carcinoma of no special type lymph nodes metastasis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c5ffa326c4764779884adfbb78fe0501
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spelling	oai:doaj.org-article:c5ffa326c4764779884adfbb78fe05012021-11-18T08:03:49ZCytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases1563-06252313-741X10.15789/1563-0625-2019-6-1115-1126https://doaj.org/article/c5ffa326c4764779884adfbb78fe05012020-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1764https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of this study was to evaluate production of cytokines by the blood immune cells in patients of different age groups with invasive non-specified type mammary ductal carcinoma, with or without metastases. Production of cytokines by periphery blood immunocompetent cells, either spontaneous and stimulated with polyclonal activators, was assessed in 82 patients with invasive mammary ductal carcinoma. The concentrations of IL-6, IL-8, IL-10, IL-17, IL-18, IL-1p, IL-1ra, TNFa, IFNy, G-CSF, GM-CSF, VEGF и MCP-1 were determined by solid-phase enzyme-linked immunosorbent assay. The patients were divided into 2 age groups: the first group consisted of 48 patients from 45 to 60 years old; the second group consisted of 34 older patients (61 to 75 years old). Metastases in local lymph nodes were documented in twenty patients from the first group and nine patients from the second group. The younger patients (45-60 years old) with metastases in local lymph nodes showed higher polyclonal activation index of IL-4 and IL-1ra production, when compared to the patients without lymphatic metastases. As for the older patients (61 to 75 years old), their polyclonal activation index of IL-6, IL-8, IL-1ra, G-CFS, GM-CSF production was significantly lower in cases of local lymph nodes metastases. The latter was due to higher level of spontaneous production, which suppressed the influence of polyclonal activators. The index of polyclonal activation upon production of cytokines in patients with lymphatic metastases was shown to be significantly higher for the age group of 45 to 60 years than in the age group of 61 to 75 years old. This fact suggested a highly stimulating effect of polyclonal activators in patients younger than 60 years. It was found that correlation between the index of polyclonal activation of cytokine production, and the indexes of ER, PR, HER2/NEU and Ki-67 receptor expression, which are used for the determination of molecular genetic subtype of the tumor, differ significantly for the distinct age groups.K. I. DavletovaE. S. MikhaylovaN. A. VaraksinL. P. ZhurakovskyA. V. ProskuraS. V. SidorovA. I. AutenshlyusSPb RAACIarticlecytokinesblood immune cellspolyclonal activatorsbreast cancerinvasive ductal carcinoma of no special typelymph nodes metastasisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 6, Pp 1115-1126 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines blood immune cells polyclonal activators breast cancer invasive ductal carcinoma of no special type lymph nodes metastasis Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines blood immune cells polyclonal activators breast cancer invasive ductal carcinoma of no special type lymph nodes metastasis Immunologic diseases. Allergy RC581-607 K. I. Davletova E. S. Mikhaylova N. A. Varaksin L. P. Zhurakovsky A. V. Proskura S. V. Sidorov A. I. Autenshlyus Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
description	The aim of this study was to evaluate production of cytokines by the blood immune cells in patients of different age groups with invasive non-specified type mammary ductal carcinoma, with or without metastases. Production of cytokines by periphery blood immunocompetent cells, either spontaneous and stimulated with polyclonal activators, was assessed in 82 patients with invasive mammary ductal carcinoma. The concentrations of IL-6, IL-8, IL-10, IL-17, IL-18, IL-1p, IL-1ra, TNFa, IFNy, G-CSF, GM-CSF, VEGF и MCP-1 were determined by solid-phase enzyme-linked immunosorbent assay. The patients were divided into 2 age groups: the first group consisted of 48 patients from 45 to 60 years old; the second group consisted of 34 older patients (61 to 75 years old). Metastases in local lymph nodes were documented in twenty patients from the first group and nine patients from the second group. The younger patients (45-60 years old) with metastases in local lymph nodes showed higher polyclonal activation index of IL-4 and IL-1ra production, when compared to the patients without lymphatic metastases. As for the older patients (61 to 75 years old), their polyclonal activation index of IL-6, IL-8, IL-1ra, G-CFS, GM-CSF production was significantly lower in cases of local lymph nodes metastases. The latter was due to higher level of spontaneous production, which suppressed the influence of polyclonal activators. The index of polyclonal activation upon production of cytokines in patients with lymphatic metastases was shown to be significantly higher for the age group of 45 to 60 years than in the age group of 61 to 75 years old. This fact suggested a highly stimulating effect of polyclonal activators in patients younger than 60 years. It was found that correlation between the index of polyclonal activation of cytokine production, and the indexes of ER, PR, HER2/NEU and Ki-67 receptor expression, which are used for the determination of molecular genetic subtype of the tumor, differ significantly for the distinct age groups.
format	article
author	K. I. Davletova E. S. Mikhaylova N. A. Varaksin L. P. Zhurakovsky A. V. Proskura S. V. Sidorov A. I. Autenshlyus
author_facet	K. I. Davletova E. S. Mikhaylova N. A. Varaksin L. P. Zhurakovsky A. V. Proskura S. V. Sidorov A. I. Autenshlyus
author_sort	K. I. Davletova
title	Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
title_short	Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
title_full	Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
title_fullStr	Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
title_full_unstemmed	Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
title_sort	cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/c5ffa326c4764779884adfbb78fe0501
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Cytokinesproduction by blood immune cells in patients of different age groups with invasive ductal carcinoma of no special type and lymphatic metastases

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