Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans

Abstract Bacteria naturally form communities of cells known as biofilms. However the physiological roles of biofilms produced by non-pathogenic microbiota remain largely unknown. To assess the impact of a biofilm on host physiology we explored the effect of several non-pathogenic biofilm-forming bac...

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Autores principales:	Olga Smolentseva, Ivan Gusarov, Laurent Gautier, Ilya Shamovsky, Alicia S. DeFrancesco, Richard Losick, Evgeny Nudler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c600dcd1bdc7436bab0da15619883072
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spelling	oai:doaj.org-article:c600dcd1bdc7436bab0da156198830722021-12-02T16:08:21ZMechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans10.1038/s41598-017-07222-82045-2322https://doaj.org/article/c600dcd1bdc7436bab0da156198830722017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07222-8https://doaj.org/toc/2045-2322Abstract Bacteria naturally form communities of cells known as biofilms. However the physiological roles of biofilms produced by non-pathogenic microbiota remain largely unknown. To assess the impact of a biofilm on host physiology we explored the effect of several non-pathogenic biofilm-forming bacteria on Caenorhabditis elegans. We show that biofilm formation by Bacillus subtilis, Lactobacillus rhamnosus and Pseudomonas fluorescens induces C. elegans stress resistance. Biofilm also protects against pathogenic infection and prolongs lifespan. Total mRNA analysis identified a set of host genes that are upregulated in response to biofilm formation by B. subtilis. We further demonstrate that mtl-1 is responsible for the biofilm-mediated increase in oxidative stress resistance and lifespan extension. Induction of mtl-1 and hsp-70 promotes biofilm-mediated thermotolerance. ilys-2 activity accounts for biofilm-mediated resistance to Pseudomonas aeruginosa killing. These results reveal the importance of non-pathogenic biofilms for host physiology and provide a framework to study commensal biofilms in higher organisms.Olga SmolentsevaIvan GusarovLaurent GautierIlya ShamovskyAlicia S. DeFrancescoRichard LosickEvgeny NudlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olga Smolentseva Ivan Gusarov Laurent Gautier Ilya Shamovsky Alicia S. DeFrancesco Richard Losick Evgeny Nudler Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
description	Abstract Bacteria naturally form communities of cells known as biofilms. However the physiological roles of biofilms produced by non-pathogenic microbiota remain largely unknown. To assess the impact of a biofilm on host physiology we explored the effect of several non-pathogenic biofilm-forming bacteria on Caenorhabditis elegans. We show that biofilm formation by Bacillus subtilis, Lactobacillus rhamnosus and Pseudomonas fluorescens induces C. elegans stress resistance. Biofilm also protects against pathogenic infection and prolongs lifespan. Total mRNA analysis identified a set of host genes that are upregulated in response to biofilm formation by B. subtilis. We further demonstrate that mtl-1 is responsible for the biofilm-mediated increase in oxidative stress resistance and lifespan extension. Induction of mtl-1 and hsp-70 promotes biofilm-mediated thermotolerance. ilys-2 activity accounts for biofilm-mediated resistance to Pseudomonas aeruginosa killing. These results reveal the importance of non-pathogenic biofilms for host physiology and provide a framework to study commensal biofilms in higher organisms.
format	article
author	Olga Smolentseva Ivan Gusarov Laurent Gautier Ilya Shamovsky Alicia S. DeFrancesco Richard Losick Evgeny Nudler
author_facet	Olga Smolentseva Ivan Gusarov Laurent Gautier Ilya Shamovsky Alicia S. DeFrancesco Richard Losick Evgeny Nudler
author_sort	Olga Smolentseva
title	Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
title_short	Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
title_full	Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
title_fullStr	Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
title_full_unstemmed	Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans
title_sort	mechanism of biofilm-mediated stress resistance and lifespan extension in c. elegans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c600dcd1bdc7436bab0da15619883072
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Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans

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