Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens

Abstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, an...

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Autores principales:	Tasuku Hiroshige, Kei-Ichiro Uemura, Shingo Hirashima, Kiyosato Hino, Akinobu Togo, Keisuke Ohta, Tsukasa Igawa, Kei-Ichiro Nakamura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c60511263b4d4fe9a8f6f45f4fa5372a
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spelling	oai:doaj.org-article:c60511263b4d4fe9a8f6f45f4fa5372a2021-12-02T14:21:16ZIdentification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens10.1038/s41598-021-87049-62045-2322https://doaj.org/article/c60511263b4d4fe9a8f6f45f4fa5372a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87049-6https://doaj.org/toc/2045-2322Abstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility.Tasuku HiroshigeKei-Ichiro UemuraShingo HirashimaKiyosato HinoAkinobu TogoKeisuke OhtaTsukasa IgawaKei-Ichiro NakamuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
description	Abstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility.
format	article
author	Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura
author_facet	Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura
author_sort	Tasuku Hiroshige
title	Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
title_short	Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
title_full	Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
title_fullStr	Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
title_full_unstemmed	Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
title_sort	identification of pdgfrα-positive interstitial cells in the distal segment of the murine vas deferens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c60511263b4d4fe9a8f6f45f4fa5372a
work_keys_str_mv	AT tasukuhiroshige identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT keiichirouemura identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT shingohirashima identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT kiyosatohino identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT akinobutogo identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT keisukeohta identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT tsukasaigawa identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens AT keiichironakamura identificationofpdgfrapositiveinterstitialcellsinthedistalsegmentofthemurinevasdeferens
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Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens

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