Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development.
The potential requirement of either the Prion or Shadoo protein for early mouse embryogenesis was recently suggested. However, the current data did not allow to precise the developmental process that was affected in the absence of both proteins and that led to the observed early lethal phenotype. In...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c607488682ee4ea6abf6da4d4c32a7a5 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c607488682ee4ea6abf6da4d4c32a7a5 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c607488682ee4ea6abf6da4d4c32a7a52021-11-18T07:10:24ZPrion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development.1932-620310.1371/journal.pone.0041959https://doaj.org/article/c607488682ee4ea6abf6da4d4c32a7a52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22860039/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The potential requirement of either the Prion or Shadoo protein for early mouse embryogenesis was recently suggested. However, the current data did not allow to precise the developmental process that was affected in the absence of both proteins and that led to the observed early lethal phenotype. In the present study, using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages. Histological analysis reveals developmental defect of the ectoplacental cone and important hemorrhage surrounding the Prnp-knockout-Sprn-knockdown E7.5 embryos. By restricting the RNA interference to the trophoblastic cell lineages, the observed lethal phenotype could be attributed to the sole role of these proteins in this trophectoderm-derived compartment. RNAseq analysis performed on early embryos of various Prnp and Sprn genotypes indicated that the simultaneous down-regulation of these two proteins affects cell-adhesion and inflammatory pathways as well as the expression of ectoplacental-specific genes. Overall, our data provide biological clues in favor of a crucial and complementary embryonic role of the prion protein family in Eutherians and emphasizes the need to further evaluate its implication in normal and pathological human placenta biology.Bruno PassetRachel YoungSamira MakhzamiMarthe VilotteFlorence JaffrezicSophie HalliezStéphan BouetSylvain MartheyManal KhaliféColette Kanellopoulos-LangevinVincent BéringueFabienne Le ProvostHubert LaudeJean-Luc VilottePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41959 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Bruno Passet Rachel Young Samira Makhzami Marthe Vilotte Florence Jaffrezic Sophie Halliez Stéphan Bouet Sylvain Marthey Manal Khalifé Colette Kanellopoulos-Langevin Vincent Béringue Fabienne Le Provost Hubert Laude Jean-Luc Vilotte Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| description |
The potential requirement of either the Prion or Shadoo protein for early mouse embryogenesis was recently suggested. However, the current data did not allow to precise the developmental process that was affected in the absence of both proteins and that led to the observed early lethal phenotype. In the present study, using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages. Histological analysis reveals developmental defect of the ectoplacental cone and important hemorrhage surrounding the Prnp-knockout-Sprn-knockdown E7.5 embryos. By restricting the RNA interference to the trophoblastic cell lineages, the observed lethal phenotype could be attributed to the sole role of these proteins in this trophectoderm-derived compartment. RNAseq analysis performed on early embryos of various Prnp and Sprn genotypes indicated that the simultaneous down-regulation of these two proteins affects cell-adhesion and inflammatory pathways as well as the expression of ectoplacental-specific genes. Overall, our data provide biological clues in favor of a crucial and complementary embryonic role of the prion protein family in Eutherians and emphasizes the need to further evaluate its implication in normal and pathological human placenta biology. |
| format |
article |
| author |
Bruno Passet Rachel Young Samira Makhzami Marthe Vilotte Florence Jaffrezic Sophie Halliez Stéphan Bouet Sylvain Marthey Manal Khalifé Colette Kanellopoulos-Langevin Vincent Béringue Fabienne Le Provost Hubert Laude Jean-Luc Vilotte |
| author_facet |
Bruno Passet Rachel Young Samira Makhzami Marthe Vilotte Florence Jaffrezic Sophie Halliez Stéphan Bouet Sylvain Marthey Manal Khalifé Colette Kanellopoulos-Langevin Vincent Béringue Fabienne Le Provost Hubert Laude Jean-Luc Vilotte |
| author_sort |
Bruno Passet |
| title |
Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| title_short |
Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| title_full |
Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| title_fullStr |
Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| title_full_unstemmed |
Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| title_sort |
prion protein and shadoo are involved in overlapping embryonic pathways and trophoblastic development. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/c607488682ee4ea6abf6da4d4c32a7a5 |
| work_keys_str_mv |
AT brunopasset prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT rachelyoung prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT samiramakhzami prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT marthevilotte prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT florencejaffrezic prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT sophiehalliez prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT stephanbouet prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT sylvainmarthey prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT manalkhalife prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT colettekanellopouloslangevin prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT vincentberingue prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT fabienneleprovost prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT hubertlaude prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment AT jeanlucvilotte prionproteinandshadooareinvolvedinoverlappingembryonicpathwaysandtrophoblasticdevelopment |
| _version_ |
1718423880518860800 |