Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection

Abstract Asthmatic patients present more rapid progression of respiratory distress after A(H1N1)pdm09 influenza infection than after seasonal infection. Here, we sought to clarify the pathophysiology of early deterioration in asthmatic patients after A(H1N1)pdm09 infection. Cytokine levels and virus...

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Autores principales:	Yousuke Fujimoto, Shunji Hasegawa, Takeshi Matsushige, Hiroyuki Wakiguchi, Tamaki Nakamura, Hideki Hasegawa, Noriko Nakajima, Akira Ainai, Atsunori Oga, Hiroshi Itoh, Komei Shirabe, Shoichi Toda, Ryo Atsuta, Tsuneo Morishima, Shouichi Ohga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6093d5352144e89825583ac1c798639
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spelling	oai:doaj.org-article:c6093d5352144e89825583ac1c7986392021-12-02T12:31:59ZPulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection10.1038/s41598-017-08030-w2045-2322https://doaj.org/article/c6093d5352144e89825583ac1c7986392017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08030-whttps://doaj.org/toc/2045-2322Abstract Asthmatic patients present more rapid progression of respiratory distress after A(H1N1)pdm09 influenza infection than after seasonal infection. Here, we sought to clarify the pathophysiology of early deterioration in asthmatic patients after A(H1N1)pdm09 infection. Cytokine levels and virus titres in bronchoalveolar lavage fluid from mice with and without asthma after A(H1N1)pdm09 or seasonal H1N1 infection were examined. In asthma/A(H1N1)pdm09 mice, IL-6 and TNF-α levels peaked at 3 days post-infection and were higher than those in all other groups. IFN-γ levels in asthma/A(H1N1)pdm09 mice at 3 days post-infection were higher than in all other mice at any time point, whereas at 7 days post-infection, the levels were lowest in asthma/A(H1N1)pdm09 mice. Virus titres in asthma/A(H1N1)pdm09 mice were highest at 3 days post-infection, and decreased by 7 days post-infection, although the levels at this time point were still higher than that in any other group. Histopathological examination showed more inflammatory cell infiltration and lung tissue destruction in the asthma/A(H1N1)pdm09 group than in any other group. The distinct cytokine profiles in A(H1N1)pdm09-infected asthmatic mice indicated excessive inflammation and virus replication within a few days after infection. Thus, bronchial asthma could be a more exacerbating factor for pandemic influenza infection than for seasonal influenza infection.Yousuke FujimotoShunji HasegawaTakeshi MatsushigeHiroyuki WakiguchiTamaki NakamuraHideki HasegawaNoriko NakajimaAkira AinaiAtsunori OgaHiroshi ItohKomei ShirabeShoichi TodaRyo AtsutaTsuneo MorishimaShouichi OhgaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yousuke Fujimoto Shunji Hasegawa Takeshi Matsushige Hiroyuki Wakiguchi Tamaki Nakamura Hideki Hasegawa Noriko Nakajima Akira Ainai Atsunori Oga Hiroshi Itoh Komei Shirabe Shoichi Toda Ryo Atsuta Tsuneo Morishima Shouichi Ohga Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
description	Abstract Asthmatic patients present more rapid progression of respiratory distress after A(H1N1)pdm09 influenza infection than after seasonal infection. Here, we sought to clarify the pathophysiology of early deterioration in asthmatic patients after A(H1N1)pdm09 infection. Cytokine levels and virus titres in bronchoalveolar lavage fluid from mice with and without asthma after A(H1N1)pdm09 or seasonal H1N1 infection were examined. In asthma/A(H1N1)pdm09 mice, IL-6 and TNF-α levels peaked at 3 days post-infection and were higher than those in all other groups. IFN-γ levels in asthma/A(H1N1)pdm09 mice at 3 days post-infection were higher than in all other mice at any time point, whereas at 7 days post-infection, the levels were lowest in asthma/A(H1N1)pdm09 mice. Virus titres in asthma/A(H1N1)pdm09 mice were highest at 3 days post-infection, and decreased by 7 days post-infection, although the levels at this time point were still higher than that in any other group. Histopathological examination showed more inflammatory cell infiltration and lung tissue destruction in the asthma/A(H1N1)pdm09 group than in any other group. The distinct cytokine profiles in A(H1N1)pdm09-infected asthmatic mice indicated excessive inflammation and virus replication within a few days after infection. Thus, bronchial asthma could be a more exacerbating factor for pandemic influenza infection than for seasonal influenza infection.
format	article
author	Yousuke Fujimoto Shunji Hasegawa Takeshi Matsushige Hiroyuki Wakiguchi Tamaki Nakamura Hideki Hasegawa Noriko Nakajima Akira Ainai Atsunori Oga Hiroshi Itoh Komei Shirabe Shoichi Toda Ryo Atsuta Tsuneo Morishima Shouichi Ohga
author_facet	Yousuke Fujimoto Shunji Hasegawa Takeshi Matsushige Hiroyuki Wakiguchi Tamaki Nakamura Hideki Hasegawa Noriko Nakajima Akira Ainai Atsunori Oga Hiroshi Itoh Komei Shirabe Shoichi Toda Ryo Atsuta Tsuneo Morishima Shouichi Ohga
author_sort	Yousuke Fujimoto
title	Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
title_short	Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
title_full	Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
title_fullStr	Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
title_full_unstemmed	Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection
title_sort	pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during a(h1n1)pdm09 influenza infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c6093d5352144e89825583ac1c798639
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Pulmonary inflammation and cytokine dynamics of bronchoalveolar lavage fluid from a mouse model of bronchial asthma during A(H1N1)pdm09 influenza infection

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