High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

Single cell RNA sequencing generates short reads from one end of a template, providing incomplete transcript coverage and limiting identification of diverse sequences such as antigen receptors. Here the authors combine long read nanopore sequencing with short read profiling of barcoded libraries to...

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Autores principales:	Mandeep Singh, Ghamdan Al-Eryani, Shaun Carswell, James M. Ferguson, James Blackburn, Kirston Barton, Daniel Roden, Fabio Luciani, Tri Giang Phan, Simon Junankar, Katherine Jackson, Christopher C. Goodnow, Martin A. Smith, Alexander Swarbrick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c60a66377d0242a7a325fe287cec34d3
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spelling	oai:doaj.org-article:c60a66377d0242a7a325fe287cec34d32021-12-02T16:57:46ZHigh-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes10.1038/s41467-019-11049-42041-1723https://doaj.org/article/c60a66377d0242a7a325fe287cec34d32019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11049-4https://doaj.org/toc/2041-1723Single cell RNA sequencing generates short reads from one end of a template, providing incomplete transcript coverage and limiting identification of diverse sequences such as antigen receptors. Here the authors combine long read nanopore sequencing with short read profiling of barcoded libraries to generate full-length antigen receptor sequences.Mandeep SinghGhamdan Al-EryaniShaun CarswellJames M. FergusonJames BlackburnKirston BartonDaniel RodenFabio LucianiTri Giang PhanSimon JunankarKatherine JacksonChristopher C. GoodnowMartin A. SmithAlexander SwarbrickNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mandeep Singh Ghamdan Al-Eryani Shaun Carswell James M. Ferguson James Blackburn Kirston Barton Daniel Roden Fabio Luciani Tri Giang Phan Simon Junankar Katherine Jackson Christopher C. Goodnow Martin A. Smith Alexander Swarbrick High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
description	Single cell RNA sequencing generates short reads from one end of a template, providing incomplete transcript coverage and limiting identification of diverse sequences such as antigen receptors. Here the authors combine long read nanopore sequencing with short read profiling of barcoded libraries to generate full-length antigen receptor sequences.
format	article
author	Mandeep Singh Ghamdan Al-Eryani Shaun Carswell James M. Ferguson James Blackburn Kirston Barton Daniel Roden Fabio Luciani Tri Giang Phan Simon Junankar Katherine Jackson Christopher C. Goodnow Martin A. Smith Alexander Swarbrick
author_facet	Mandeep Singh Ghamdan Al-Eryani Shaun Carswell James M. Ferguson James Blackburn Kirston Barton Daniel Roden Fabio Luciani Tri Giang Phan Simon Junankar Katherine Jackson Christopher C. Goodnow Martin A. Smith Alexander Swarbrick
author_sort	Mandeep Singh
title	High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
title_short	High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
title_full	High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
title_fullStr	High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
title_full_unstemmed	High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
title_sort	high-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c60a66377d0242a7a325fe287cec34d3
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High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

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