Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture

Abstract Ovulation is dependent upon numerous factors mediating follicular growth, vascularization, and ultimately oocyte release via follicle rupture. Endothelin-2 (EDN2) is a potent vasoconstrictor that is transiently produced prior to follicle rupture by granulosa cells of periovulatory follicles...

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Autores principales:	Joseph A. Cacioppo, Po-Ching Patrick Lin, Patrick R. Hannon, Daniel R. McDougle, Arnon Gal, CheMyong Ko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c60aa4525a74446b848c5da148aeb77f
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spelling	oai:doaj.org-article:c60aa4525a74446b848c5da148aeb77f2021-12-02T11:52:42ZGranulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture10.1038/s41598-017-00943-w2045-2322https://doaj.org/article/c60aa4525a74446b848c5da148aeb77f2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00943-whttps://doaj.org/toc/2045-2322Abstract Ovulation is dependent upon numerous factors mediating follicular growth, vascularization, and ultimately oocyte release via follicle rupture. Endothelin-2 (EDN2) is a potent vasoconstrictor that is transiently produced prior to follicle rupture by granulosa cells of periovulatory follicles and induces ovarian contraction. To determine the role of Edn2 expression, surgical transplant and novel conditional knockout mice were super-ovulated and analyzed. Conditional knockout mice utilized a new iCre driven by the Esr2 promoter to selectively remove Edn2. Follicle rupture and fertility were significantly impaired in the absence of ovarian Edn2 expression. When ovaries of Edn2KO mice were transplanted in wild type recipients, significantly more corpora lutea containing un-ovulated oocytes were present after hormonal stimulation (1.0 vs. 5.4, p = 0.010). Following selective ablation of Edn2 in granulosa cells, Esr2-Edn2KO dams had reduced oocytes ovulated (3.8 vs. 16.4 oocytes/ovary) and smaller litters (4.29 ± l.02 vs. 8.50 pups/dam). However, the number of pregnancies per pairing was not different and the reproductive axis remained intact. Esr2-Edn2KO ovaries had a higher percentage of antral follicles and fewer corpora lutea; follicles progressed to the antral stage but many were unable to rupture. Conditional loss of endothelin receptor A in granulosa cells also decreased ovulation but did not affect fecundity. These data demonstrate that EDN2-induced intraovarian contraction is a critical trigger of normal ovulation and subsequent fecundity.Joseph A. CacioppoPo-Ching Patrick LinPatrick R. HannonDaniel R. McDougleArnon GalCheMyong KoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph A. Cacioppo Po-Ching Patrick Lin Patrick R. Hannon Daniel R. McDougle Arnon Gal CheMyong Ko Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
description	Abstract Ovulation is dependent upon numerous factors mediating follicular growth, vascularization, and ultimately oocyte release via follicle rupture. Endothelin-2 (EDN2) is a potent vasoconstrictor that is transiently produced prior to follicle rupture by granulosa cells of periovulatory follicles and induces ovarian contraction. To determine the role of Edn2 expression, surgical transplant and novel conditional knockout mice were super-ovulated and analyzed. Conditional knockout mice utilized a new iCre driven by the Esr2 promoter to selectively remove Edn2. Follicle rupture and fertility were significantly impaired in the absence of ovarian Edn2 expression. When ovaries of Edn2KO mice were transplanted in wild type recipients, significantly more corpora lutea containing un-ovulated oocytes were present after hormonal stimulation (1.0 vs. 5.4, p = 0.010). Following selective ablation of Edn2 in granulosa cells, Esr2-Edn2KO dams had reduced oocytes ovulated (3.8 vs. 16.4 oocytes/ovary) and smaller litters (4.29 ± l.02 vs. 8.50 pups/dam). However, the number of pregnancies per pairing was not different and the reproductive axis remained intact. Esr2-Edn2KO ovaries had a higher percentage of antral follicles and fewer corpora lutea; follicles progressed to the antral stage but many were unable to rupture. Conditional loss of endothelin receptor A in granulosa cells also decreased ovulation but did not affect fecundity. These data demonstrate that EDN2-induced intraovarian contraction is a critical trigger of normal ovulation and subsequent fecundity.
format	article
author	Joseph A. Cacioppo Po-Ching Patrick Lin Patrick R. Hannon Daniel R. McDougle Arnon Gal CheMyong Ko
author_facet	Joseph A. Cacioppo Po-Ching Patrick Lin Patrick R. Hannon Daniel R. McDougle Arnon Gal CheMyong Ko
author_sort	Joseph A. Cacioppo
title	Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
title_short	Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
title_full	Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
title_fullStr	Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
title_full_unstemmed	Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
title_sort	granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c60aa4525a74446b848c5da148aeb77f
work_keys_str_mv	AT josephacacioppo granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture AT pochingpatricklin granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture AT patrickrhannon granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture AT danielrmcdougle granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture AT arnongal granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture AT chemyongko granulosacellendothelin2expressionisfundamentalforovulatoryfolliclerupture
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Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture

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