Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking
Summary: This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins. We then use live-cell single-molecule tracking to obtain kinetic populations and...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/c614c8de3f504d0cac585ccee3d1f128 |
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