Chemically different non-thermal plasmas target distinct cell death pathways

Abstract A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically dif...

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Autores principales:	Oleg Lunov, Vitalii Zablotskii, Olexander Churpita, Mariia Lunova, Milan Jirsa, Alexandr Dejneka, Šárka Kubinová
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c61cc329adb748a786c91705bc13e614
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spelling	oai:doaj.org-article:c61cc329adb748a786c91705bc13e6142021-12-02T16:06:46ZChemically different non-thermal plasmas target distinct cell death pathways10.1038/s41598-017-00689-52045-2322https://doaj.org/article/c61cc329adb748a786c91705bc13e6142017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00689-5https://doaj.org/toc/2045-2322Abstract A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.Oleg LunovVitalii ZablotskiiOlexander ChurpitaMariia LunovaMilan JirsaAlexandr DejnekaŠárka KubinováNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oleg Lunov Vitalii Zablotskii Olexander Churpita Mariia Lunova Milan Jirsa Alexandr Dejneka Šárka Kubinová Chemically different non-thermal plasmas target distinct cell death pathways
description	Abstract A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
format	article
author	Oleg Lunov Vitalii Zablotskii Olexander Churpita Mariia Lunova Milan Jirsa Alexandr Dejneka Šárka Kubinová
author_facet	Oleg Lunov Vitalii Zablotskii Olexander Churpita Mariia Lunova Milan Jirsa Alexandr Dejneka Šárka Kubinová
author_sort	Oleg Lunov
title	Chemically different non-thermal plasmas target distinct cell death pathways
title_short	Chemically different non-thermal plasmas target distinct cell death pathways
title_full	Chemically different non-thermal plasmas target distinct cell death pathways
title_fullStr	Chemically different non-thermal plasmas target distinct cell death pathways
title_full_unstemmed	Chemically different non-thermal plasmas target distinct cell death pathways
title_sort	chemically different non-thermal plasmas target distinct cell death pathways
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c61cc329adb748a786c91705bc13e614
work_keys_str_mv	AT oleglunov chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT vitaliizablotskii chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT olexanderchurpita chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT mariialunova chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT milanjirsa chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT alexandrdejneka chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways AT sarkakubinova chemicallydifferentnonthermalplasmastargetdistinctcelldeathpathways
_version_	1718384868303306752

Chemically different non-thermal plasmas target distinct cell death pathways

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