Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1

Abstract The severity of tissue injury in burn wounds from associated inflammatory and immune sequelae presents a significant clinical management challenge. Among various biophysical wound management approaches, low dose biophotonics treatments, termed Photobiomodulation (PBM) therapy, has gained re...

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Autores principales:	Imran Khan, Saeed Ur Rahman, Elieza Tang, Karl Engel, Bradford Hall, Ashok B. Kulkarni, Praveen R. Arany
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c623ab933bc9406d9c1b87954e06c0fd
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spelling	oai:doaj.org-article:c623ab933bc9406d9c1b87954e06c0fd2021-12-02T18:18:44ZAccelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β110.1038/s41598-021-92650-w2045-2322https://doaj.org/article/c623ab933bc9406d9c1b87954e06c0fd2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92650-whttps://doaj.org/toc/2045-2322Abstract The severity of tissue injury in burn wounds from associated inflammatory and immune sequelae presents a significant clinical management challenge. Among various biophysical wound management approaches, low dose biophotonics treatments, termed Photobiomodulation (PBM) therapy, has gained recent attention. One of the PBM molecular mechanisms of PBM treatments involves photoactivation of latent TGF-β1 that is capable of promoting tissue healing and regeneration. This work examined the efficacy of PBM treatments in a full-thickness burn wound healing in C57BL/6 mice. We first optimized the PBM protocol by monitoring tissue surface temperature and histology. We noted this dynamic irradiance surface temperature-monitored PBM protocol improved burn wound healing in mice with elevated TGF-β signaling (phospho-Smad2) and reduced inflammation-associated gene expression. Next, we investigated the roles of individual cell types involved in burn wound healing following PBM treatments and noted discrete effects on epithelieum, fibroblasts, and macrophage functions. These responses appear to be mediated via both TGF-β dependent and independent signaling pathways. Finally, to investigate specific contributions of TGF-β1 signaling in these PBM-burn wound healing, we utilized a chimeric TGF-β1/β3 knock-in (TGF-β1Lβ3/Lβ3) mice. PBM treatments failed to activate the chimeric TGF-β1Lβ3/Lβ3 complex and failed to improve burn wound healing in these mice. These results suggest activation of endogenous latent TGF-β1 following PBM treatments plays a key role in burn wound healing. These mechanistic insights can improve the safety and efficacy of clinical translation of PBM treatments for tissue healing and regeneration.Imran KhanSaeed Ur RahmanElieza TangKarl EngelBradford HallAshok B. KulkarniPraveen R. AranyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Imran Khan Saeed Ur Rahman Elieza Tang Karl Engel Bradford Hall Ashok B. Kulkarni Praveen R. Arany Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
description	Abstract The severity of tissue injury in burn wounds from associated inflammatory and immune sequelae presents a significant clinical management challenge. Among various biophysical wound management approaches, low dose biophotonics treatments, termed Photobiomodulation (PBM) therapy, has gained recent attention. One of the PBM molecular mechanisms of PBM treatments involves photoactivation of latent TGF-β1 that is capable of promoting tissue healing and regeneration. This work examined the efficacy of PBM treatments in a full-thickness burn wound healing in C57BL/6 mice. We first optimized the PBM protocol by monitoring tissue surface temperature and histology. We noted this dynamic irradiance surface temperature-monitored PBM protocol improved burn wound healing in mice with elevated TGF-β signaling (phospho-Smad2) and reduced inflammation-associated gene expression. Next, we investigated the roles of individual cell types involved in burn wound healing following PBM treatments and noted discrete effects on epithelieum, fibroblasts, and macrophage functions. These responses appear to be mediated via both TGF-β dependent and independent signaling pathways. Finally, to investigate specific contributions of TGF-β1 signaling in these PBM-burn wound healing, we utilized a chimeric TGF-β1/β3 knock-in (TGF-β1Lβ3/Lβ3) mice. PBM treatments failed to activate the chimeric TGF-β1Lβ3/Lβ3 complex and failed to improve burn wound healing in these mice. These results suggest activation of endogenous latent TGF-β1 following PBM treatments plays a key role in burn wound healing. These mechanistic insights can improve the safety and efficacy of clinical translation of PBM treatments for tissue healing and regeneration.
format	article
author	Imran Khan Saeed Ur Rahman Elieza Tang Karl Engel Bradford Hall Ashok B. Kulkarni Praveen R. Arany
author_facet	Imran Khan Saeed Ur Rahman Elieza Tang Karl Engel Bradford Hall Ashok B. Kulkarni Praveen R. Arany
author_sort	Imran Khan
title	Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
title_short	Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
title_full	Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
title_fullStr	Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
title_full_unstemmed	Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1
title_sort	accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent tgf-β1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c623ab933bc9406d9c1b87954e06c0fd
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Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1

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