Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.

The normal growth of mycobacteria attributes to the integrity of cell wall core which consists of peptidoglycan (PG), arabinogalactan (AG) and mycolic acids. N-acetyl glucosamine (GlcNAc) is an essential component in both PG and AG of mycobacterial cell wall. The biosynthetic pathway for UDP-N-acety...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shuang Li, Jian Kang, Wendan Yu, Yan Zhou, Wenli Zhang, Yi Xin, Yufang Ma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c62a95bc95f2436eac3272c5fcf8b274
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c62a95bc95f2436eac3272c5fcf8b274
record_format	dspace
spelling	oai:doaj.org-article:c62a95bc95f2436eac3272c5fcf8b2742021-11-18T07:09:18ZIdentification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.1932-620310.1371/journal.pone.0042769https://doaj.org/article/c62a95bc95f2436eac3272c5fcf8b2742012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905172/?tool=EBIhttps://doaj.org/toc/1932-6203The normal growth of mycobacteria attributes to the integrity of cell wall core which consists of peptidoglycan (PG), arabinogalactan (AG) and mycolic acids. N-acetyl glucosamine (GlcNAc) is an essential component in both PG and AG of mycobacterial cell wall. The biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc), as a sugar donor of GlcNAc, is different in prokaryotes and eukaryotes. The conversion of glucosamine-6-phosphate to glucosamine-1-phosphate, which is catalyzed by phosphoglucosamine mutase (GlmM), is unique to prokaryotes. Bioinformatic analysis showed that Msm MSMEG_1556 and Mtb Rv3441c are homologous to Ec GlmM. In this study, soluble Msm MSMEG_1556 protein and Mtb Rv3441c protein were expressed in E. coli BL21(DE3) and their phosphoglucosamine mutase activity were detected. In order to further investigate the essentiality of MSMEG_1556 for the growth of M. smegmatis, we generated a conditional MSMEG_1556 knockout mutant, which harbored thermo-sensitive rescue plasmid carrying Mtb Rv3441c. As the rescue plasmid was unable to complement MSMEG_1556 deficiency at 42 °C, MSMEG_1556 knockout mutant did not grow. The dramatic morphological changes of MSMEG_1556 knockout mutant after temperature shift from 30 °C to 42 °C have been observed by scanning electron microscope. These results demonstrated that MSMEG_1556 is essential for growth of M. smegmatis. This study provided evidence that GlmM enzyme could be as a potential target for developing anti-tuberculosis drugs.Shuang LiJian KangWendan YuYan ZhouWenli ZhangYi XinYufang MaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42769 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuang Li Jian Kang Wendan Yu Yan Zhou Wenli Zhang Yi Xin Yufang Ma Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
description	The normal growth of mycobacteria attributes to the integrity of cell wall core which consists of peptidoglycan (PG), arabinogalactan (AG) and mycolic acids. N-acetyl glucosamine (GlcNAc) is an essential component in both PG and AG of mycobacterial cell wall. The biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc), as a sugar donor of GlcNAc, is different in prokaryotes and eukaryotes. The conversion of glucosamine-6-phosphate to glucosamine-1-phosphate, which is catalyzed by phosphoglucosamine mutase (GlmM), is unique to prokaryotes. Bioinformatic analysis showed that Msm MSMEG_1556 and Mtb Rv3441c are homologous to Ec GlmM. In this study, soluble Msm MSMEG_1556 protein and Mtb Rv3441c protein were expressed in E. coli BL21(DE3) and their phosphoglucosamine mutase activity were detected. In order to further investigate the essentiality of MSMEG_1556 for the growth of M. smegmatis, we generated a conditional MSMEG_1556 knockout mutant, which harbored thermo-sensitive rescue plasmid carrying Mtb Rv3441c. As the rescue plasmid was unable to complement MSMEG_1556 deficiency at 42 °C, MSMEG_1556 knockout mutant did not grow. The dramatic morphological changes of MSMEG_1556 knockout mutant after temperature shift from 30 °C to 42 °C have been observed by scanning electron microscope. These results demonstrated that MSMEG_1556 is essential for growth of M. smegmatis. This study provided evidence that GlmM enzyme could be as a potential target for developing anti-tuberculosis drugs.
format	article
author	Shuang Li Jian Kang Wendan Yu Yan Zhou Wenli Zhang Yi Xin Yufang Ma
author_facet	Shuang Li Jian Kang Wendan Yu Yan Zhou Wenli Zhang Yi Xin Yufang Ma
author_sort	Shuang Li
title	Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
title_short	Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
title_full	Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
title_fullStr	Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
title_full_unstemmed	Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.
title_sort	identification of m. tuberculosis rv3441c and m. smegmatis msmeg_1556 and essentiality of m. smegmatis msmeg_1556.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c62a95bc95f2436eac3272c5fcf8b274
work_keys_str_mv	AT shuangli identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT jiankang identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT wendanyu identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT yanzhou identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT wenlizhang identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT yixin identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556 AT yufangma identificationofmtuberculosisrv3441candmsmegmatismsmeg1556andessentialityofmsmegmatismsmeg1556
_version_	1718423880927805440

Identification of M. tuberculosis Rv3441c and M. smegmatis MSMEG_1556 and essentiality of M. smegmatis MSMEG_1556.

Ejemplares similares