VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

Chronic skin ulcers are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. Adipose-derived stem cells (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with A...

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Autores principales:	Wen Jiang, Jun Zhang, Xudong Zhang, Chenghong Fan, Jinlong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	adipose-derived stem cells (adscs) vap-plga microspheres (vap-plga) chronic skin ulcers wound healing pi3k/akt/hif-1α pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/c62dc5ec886942b3b8f3715218d691ee
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spelling	oai:doaj.org-article:c62dc5ec886942b3b8f3715218d691ee2021-12-01T14:41:00ZVAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway2165-59792165-598710.1080/21655979.2021.1990193https://doaj.org/article/c62dc5ec886942b3b8f3715218d691ee2021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1990193https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Chronic skin ulcers are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. Adipose-derived stem cells (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with ADSCs in wound healing of chronic skin ulcers. The effect of VAP on phenotypes of ADSCs, and VAP (PLGA microspheres) combining with ADSCs on wound healing of chronic skin ulcers in vivo was evaluated. VAP generally promoted the proliferation, migration and invasion of ADSCs, and ADSC-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) through PI3K/Akt/HIF-1α pathway. VAP-PLGA (PLGA microspheres) enhanced the promoting effect of ADSCs on wound healing, pathological changes, and angiogenesis in chronic skin ulcers in vivo. VAP-PLGA intensified the effect of ADSCs on up-regulating the levels of p-PI3K/PI3K, p-Akt/Akt, HIF-1α, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), C-X-C motif chemokine receptor 4 (CXCR4), angiopoietin-4 (Ang-4), VEGF receptor (VEGFR), and transforming growth factor-β1 (TGF-β1), and down-regulating the levels of interleukin-1 β (IL-1β), IL-18 and IL-6 in wound tissues in chronic skin ulcers in vivo. Collectively, VAP promoted the growth, migration, invasion, and angiogenesis of ADSCs through activating PI3K/Akt/HIF-1α pathway, and VAP-PLGA enhanced the function of ADSCs in promoting wound healing in vivo, which was associated with angiogenesis, inflammation inhibition, and dermal collagen synthesis.Wen JiangJun ZhangXudong ZhangChenghong FanJinlong HuangTaylor & Francis Grouparticleadipose-derived stem cells (adscs)vap-plga microspheres (vap-plga)chronic skin ulcerswound healingpi3k/akt/hif-1α pathwayBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10264-10284 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose-derived stem cells (adscs) vap-plga microspheres (vap-plga) chronic skin ulcers wound healing pi3k/akt/hif-1α pathway Biotechnology TP248.13-248.65
spellingShingle	adipose-derived stem cells (adscs) vap-plga microspheres (vap-plga) chronic skin ulcers wound healing pi3k/akt/hif-1α pathway Biotechnology TP248.13-248.65 Wen Jiang Jun Zhang Xudong Zhang Chenghong Fan Jinlong Huang VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
description	Chronic skin ulcers are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. Adipose-derived stem cells (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with ADSCs in wound healing of chronic skin ulcers. The effect of VAP on phenotypes of ADSCs, and VAP (PLGA microspheres) combining with ADSCs on wound healing of chronic skin ulcers in vivo was evaluated. VAP generally promoted the proliferation, migration and invasion of ADSCs, and ADSC-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) through PI3K/Akt/HIF-1α pathway. VAP-PLGA (PLGA microspheres) enhanced the promoting effect of ADSCs on wound healing, pathological changes, and angiogenesis in chronic skin ulcers in vivo. VAP-PLGA intensified the effect of ADSCs on up-regulating the levels of p-PI3K/PI3K, p-Akt/Akt, HIF-1α, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), C-X-C motif chemokine receptor 4 (CXCR4), angiopoietin-4 (Ang-4), VEGF receptor (VEGFR), and transforming growth factor-β1 (TGF-β1), and down-regulating the levels of interleukin-1 β (IL-1β), IL-18 and IL-6 in wound tissues in chronic skin ulcers in vivo. Collectively, VAP promoted the growth, migration, invasion, and angiogenesis of ADSCs through activating PI3K/Akt/HIF-1α pathway, and VAP-PLGA enhanced the function of ADSCs in promoting wound healing in vivo, which was associated with angiogenesis, inflammation inhibition, and dermal collagen synthesis.
format	article
author	Wen Jiang Jun Zhang Xudong Zhang Chenghong Fan Jinlong Huang
author_facet	Wen Jiang Jun Zhang Xudong Zhang Chenghong Fan Jinlong Huang
author_sort	Wen Jiang
title	VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
title_short	VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
title_full	VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
title_fullStr	VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
title_full_unstemmed	VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway
title_sort	vap-plga microspheres (vap-plga) promote adipose-derived stem cells (adscs)-induced wound healing in chronic skin ulcers in mice via pi3k/akt/hif-1α pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/c62dc5ec886942b3b8f3715218d691ee
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VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

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