Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ

Natalie C Rollick,1 Devin B Lemmex,1 Yohei Ono,1,2 Carol R Reno,1 David A Hart,1 Ian KY Lo,1 Gail M Thornton1,3 1McCaig Institute for Bone and Joint Health, Section of Orthopaedic Surgery, Department of Surgery, University of Calgary, Calgary, AB, Canada; 2Department of Orthopaedic Surgery, Nagoya...

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Autores principales:	Rollick NC, Lemmex DB, Ono Y, Reno CR, Hart DA, Lo IK, Thornton GM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Knee Aging Surgical Menopause Joint as an Organ Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/c6321e79a72047fb981ee923ceb24d12
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spelling	oai:doaj.org-article:c6321e79a72047fb981ee923ceb24d122021-12-02T00:42:52ZGene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ1178-1998https://doaj.org/article/c6321e79a72047fb981ee923ceb24d122018-03-01T00:00:00Zhttps://www.dovepress.com/gene-expression-changes-in-knee-joint-tissues-with-aging-and-menopause-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Natalie C Rollick,1 Devin B Lemmex,1 Yohei Ono,1,2 Carol R Reno,1 David A Hart,1 Ian KY Lo,1 Gail M Thornton1,3 1McCaig Institute for Bone and Joint Health, Section of Orthopaedic Surgery, Department of Surgery, University of Calgary, Calgary, AB, Canada; 2Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan; 3Department of Orthopaedics, University of British Columbia, Vancouver, BC, Canada Background: When considering the “joint as an organ”, the tissues in a joint act as complementary components of an organ, and the “set point” is the cellular activity for homeostasis of the joint tissues. Even in the absence of injury, joint tissues have adaptive responses to processes, like aging and menopause, which result in changes to the set point.Purpose: The purpose of this study in a preclinical model was to investigate age-related and menopause-related changes in knee-joint tissues with the hypothesis that tissues will change in unique ways that reflect their differing contributions to maintaining joint function (as measured by joint laxity) and the differing processes of aging and menopause.Methods: Rabbit knee-joint tissues from three groups were evaluated: young adult (gene expression, n=8; joint laxity, n=7; water content, n=8), aging adult (gene expression, n=6; joint laxity, n=7; water content, n=5), and menopausal adult (gene expression, n=8; joint laxity, n=7; water content, n=8). Surgical menopause was induced with ovariohysterectomy surgery and gene expression was assessed using reverse-transcription quantitative polymerase chain reaction.Results: Aging resulted in changes to 37 of the 150 gene–tissue combinations evaluated, and menopause resulted in changes to 39 of the 150. Despite the similar number of changes, only eleven changes were the same in both aging and menopause. No differences in joint laxity were detected comparing young adult rabbits with aging adult rabbits or with menopausal adult rabbits.Conclusion: Aging and menopause affected the gene-expression patterns of the tissues of the knee joint differently, suggesting unique changes to the set point of the knee. Interestingly, aging and menopause did not affect knee-joint laxity, suggesting that joint function was maintained, despite changes in gene expression. Taken together, these findings support the theory of the joint as an organ where the tissues of the joint adapt to maintain joint function. Keywords: knee, aging, surgical menopause, joint as an organRollick NCLemmex DBOno YReno CRHart DALo IKThornton GMDove Medical PressarticleKneeAgingSurgical MenopauseJoint as an OrganGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 13, Pp 365-375 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Knee Aging Surgical Menopause Joint as an Organ Geriatrics RC952-954.6
spellingShingle	Knee Aging Surgical Menopause Joint as an Organ Geriatrics RC952-954.6 Rollick NC Lemmex DB Ono Y Reno CR Hart DA Lo IK Thornton GM Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
description	Natalie C Rollick,1 Devin B Lemmex,1 Yohei Ono,1,2 Carol R Reno,1 David A Hart,1 Ian KY Lo,1 Gail M Thornton1,3 1McCaig Institute for Bone and Joint Health, Section of Orthopaedic Surgery, Department of Surgery, University of Calgary, Calgary, AB, Canada; 2Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan; 3Department of Orthopaedics, University of British Columbia, Vancouver, BC, Canada Background: When considering the “joint as an organ”, the tissues in a joint act as complementary components of an organ, and the “set point” is the cellular activity for homeostasis of the joint tissues. Even in the absence of injury, joint tissues have adaptive responses to processes, like aging and menopause, which result in changes to the set point.Purpose: The purpose of this study in a preclinical model was to investigate age-related and menopause-related changes in knee-joint tissues with the hypothesis that tissues will change in unique ways that reflect their differing contributions to maintaining joint function (as measured by joint laxity) and the differing processes of aging and menopause.Methods: Rabbit knee-joint tissues from three groups were evaluated: young adult (gene expression, n=8; joint laxity, n=7; water content, n=8), aging adult (gene expression, n=6; joint laxity, n=7; water content, n=5), and menopausal adult (gene expression, n=8; joint laxity, n=7; water content, n=8). Surgical menopause was induced with ovariohysterectomy surgery and gene expression was assessed using reverse-transcription quantitative polymerase chain reaction.Results: Aging resulted in changes to 37 of the 150 gene–tissue combinations evaluated, and menopause resulted in changes to 39 of the 150. Despite the similar number of changes, only eleven changes were the same in both aging and menopause. No differences in joint laxity were detected comparing young adult rabbits with aging adult rabbits or with menopausal adult rabbits.Conclusion: Aging and menopause affected the gene-expression patterns of the tissues of the knee joint differently, suggesting unique changes to the set point of the knee. Interestingly, aging and menopause did not affect knee-joint laxity, suggesting that joint function was maintained, despite changes in gene expression. Taken together, these findings support the theory of the joint as an organ where the tissues of the joint adapt to maintain joint function. Keywords: knee, aging, surgical menopause, joint as an organ
format	article
author	Rollick NC Lemmex DB Ono Y Reno CR Hart DA Lo IK Thornton GM
author_facet	Rollick NC Lemmex DB Ono Y Reno CR Hart DA Lo IK Thornton GM
author_sort	Rollick NC
title	Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
title_short	Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
title_full	Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
title_fullStr	Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
title_full_unstemmed	Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
title_sort	gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c6321e79a72047fb981ee923ceb24d12
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Gene-expression changes in knee-joint tissues with aging and menopause: implications for the joint as an organ

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