SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.

Pathogenesis studies of SIV infection have not been performed to date in wild monkeys due to difficulty in collecting and storing samples on site and the lack of analytical reagents covering the extensive SIV diversity. We performed a large scale study of molecular epidemiology and natural history o...

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Autores principales: Dongzhu Ma, Anna Jasinska, Jan Kristoff, J Paul Grobler, Trudy Turner, Yoon Jung, Christopher Schmitt, Kevin Raehtz, Felix Feyertag, Natalie Martinez Sosa, Viskam Wijewardana, Donald S Burke, David L Robertson, Russell Tracy, Ivona Pandrea, Nelson Freimer, Cristian Apetrei, International Vervet Research Consortium
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/c633aca1400b4fc9b0bf4666627bedb6
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spelling oai:doaj.org-article:c633aca1400b4fc9b0bf4666627bedb62021-11-18T06:06:09ZSIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.1553-73661553-737410.1371/journal.ppat.1003011https://doaj.org/article/c633aca1400b4fc9b0bf4666627bedb62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23349627/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Pathogenesis studies of SIV infection have not been performed to date in wild monkeys due to difficulty in collecting and storing samples on site and the lack of analytical reagents covering the extensive SIV diversity. We performed a large scale study of molecular epidemiology and natural history of SIVagm infection in 225 free-ranging AGMs from multiple locations in South Africa. SIV prevalence (established by sequencing pol, env, and gag) varied dramatically between infant/juvenile (7%) and adult animals (68%) (p<0.0001), and between adult females (78%) and males (57%). Phylogenetic analyses revealed an extensive genetic diversity, including frequent recombination events. Some AGMs harbored epidemiologically linked viruses. Viruses infecting AGMs in the Free State, which are separated from those on the coastal side by the Drakensberg Mountains, formed a separate cluster in the phylogenetic trees; this observation supports a long standing presence of SIV in AGMs, at least from the time of their speciation to their Plio-Pleistocene migration. Specific primers/probes were synthesized based on the pol sequence data and viral loads (VLs) were quantified. VLs were of 10(4)-10(6) RNA copies/ml, in the range of those observed in experimentally-infected monkeys, validating the experimental approaches in natural hosts. VLs were significantly higher (10(7)-10(8) RNA copies/ml) in 10 AGMs diagnosed as acutely infected based on SIV seronegativity (Fiebig II), which suggests a very active transmission of SIVagm in the wild. Neither cytokine levels (as biomarkers of immune activation) nor sCD14 levels (a biomarker of microbial translocation) were different between SIV-infected and SIV-uninfected monkeys. This complex algorithm combining sequencing and phylogeny, VL quantification, serology, and testing of surrogate markers of microbial translocation and immune activation permits a systematic investigation of the epidemiology, viral diversity and natural history of SIV infection in wild African natural hosts.Dongzhu MaAnna JasinskaJan KristoffJ Paul GroblerTrudy TurnerYoon JungChristopher SchmittKevin RaehtzFelix FeyertagNatalie Martinez SosaViskam WijewardanaDonald S BurkeDavid L RobertsonRussell TracyIvona PandreaNelson FreimerCristian ApetreiInternational Vervet Research ConsortiumPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003011 (2013)
institution DOAJ
collection DOAJ
language EN
topic Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
spellingShingle Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
Dongzhu Ma
Anna Jasinska
Jan Kristoff
J Paul Grobler
Trudy Turner
Yoon Jung
Christopher Schmitt
Kevin Raehtz
Felix Feyertag
Natalie Martinez Sosa
Viskam Wijewardana
Donald S Burke
David L Robertson
Russell Tracy
Ivona Pandrea
Nelson Freimer
Cristian Apetrei
International Vervet Research Consortium
SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
description Pathogenesis studies of SIV infection have not been performed to date in wild monkeys due to difficulty in collecting and storing samples on site and the lack of analytical reagents covering the extensive SIV diversity. We performed a large scale study of molecular epidemiology and natural history of SIVagm infection in 225 free-ranging AGMs from multiple locations in South Africa. SIV prevalence (established by sequencing pol, env, and gag) varied dramatically between infant/juvenile (7%) and adult animals (68%) (p<0.0001), and between adult females (78%) and males (57%). Phylogenetic analyses revealed an extensive genetic diversity, including frequent recombination events. Some AGMs harbored epidemiologically linked viruses. Viruses infecting AGMs in the Free State, which are separated from those on the coastal side by the Drakensberg Mountains, formed a separate cluster in the phylogenetic trees; this observation supports a long standing presence of SIV in AGMs, at least from the time of their speciation to their Plio-Pleistocene migration. Specific primers/probes were synthesized based on the pol sequence data and viral loads (VLs) were quantified. VLs were of 10(4)-10(6) RNA copies/ml, in the range of those observed in experimentally-infected monkeys, validating the experimental approaches in natural hosts. VLs were significantly higher (10(7)-10(8) RNA copies/ml) in 10 AGMs diagnosed as acutely infected based on SIV seronegativity (Fiebig II), which suggests a very active transmission of SIVagm in the wild. Neither cytokine levels (as biomarkers of immune activation) nor sCD14 levels (a biomarker of microbial translocation) were different between SIV-infected and SIV-uninfected monkeys. This complex algorithm combining sequencing and phylogeny, VL quantification, serology, and testing of surrogate markers of microbial translocation and immune activation permits a systematic investigation of the epidemiology, viral diversity and natural history of SIV infection in wild African natural hosts.
format article
author Dongzhu Ma
Anna Jasinska
Jan Kristoff
J Paul Grobler
Trudy Turner
Yoon Jung
Christopher Schmitt
Kevin Raehtz
Felix Feyertag
Natalie Martinez Sosa
Viskam Wijewardana
Donald S Burke
David L Robertson
Russell Tracy
Ivona Pandrea
Nelson Freimer
Cristian Apetrei
International Vervet Research Consortium
author_facet Dongzhu Ma
Anna Jasinska
Jan Kristoff
J Paul Grobler
Trudy Turner
Yoon Jung
Christopher Schmitt
Kevin Raehtz
Felix Feyertag
Natalie Martinez Sosa
Viskam Wijewardana
Donald S Burke
David L Robertson
Russell Tracy
Ivona Pandrea
Nelson Freimer
Cristian Apetrei
International Vervet Research Consortium
author_sort Dongzhu Ma
title SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
title_short SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
title_full SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
title_fullStr SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
title_full_unstemmed SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations.
title_sort sivagm infection in wild african green monkeys from south africa: epidemiology, natural history, and evolutionary considerations.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/c633aca1400b4fc9b0bf4666627bedb6
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